Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline

X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implem...

Full description

Bibliographic Details
Main Authors: Simone Sala, Yuhe Zhang, Nathaly De La Rosa, Till Dreier, Maik Kahnt, Max Langer, Lars B. Dahlin, Martin Bech, Pablo Villanueva-Perez, Sebastian Kalbfleisch
Format: Article
Language:English
Published: International Union of Crystallography 2022-05-01
Series:Journal of Synchrotron Radiation
Subjects:
Online Access:http://scripts.iucr.org/cgi-bin/paper?S1600577522001874
Description
Summary:X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view.
ISSN:1600-5775