Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline
X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implem...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
International Union of Crystallography
2022-05-01
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| Series: | Journal of Synchrotron Radiation |
| Subjects: | |
| Online Access: | http://scripts.iucr.org/cgi-bin/paper?S1600577522001874 |
| _version_ | 1818193501763928064 |
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| author | Simone Sala Yuhe Zhang Nathaly De La Rosa Till Dreier Maik Kahnt Max Langer Lars B. Dahlin Martin Bech Pablo Villanueva-Perez Sebastian Kalbfleisch |
| author_facet | Simone Sala Yuhe Zhang Nathaly De La Rosa Till Dreier Maik Kahnt Max Langer Lars B. Dahlin Martin Bech Pablo Villanueva-Perez Sebastian Kalbfleisch |
| author_sort | Simone Sala |
| collection | DOAJ |
| description | X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view. |
| first_indexed | 2024-12-12T00:47:24Z |
| format | Article |
| id | doaj.art-9f8cc175c334437eb79fe09a1e8787f7 |
| institution | Directory Open Access Journal |
| issn | 1600-5775 |
| language | English |
| last_indexed | 2024-12-12T00:47:24Z |
| publishDate | 2022-05-01 |
| publisher | International Union of Crystallography |
| record_format | Article |
| series | Journal of Synchrotron Radiation |
| spelling | doaj.art-9f8cc175c334437eb79fe09a1e8787f72022-12-22T00:44:06ZengInternational Union of CrystallographyJournal of Synchrotron Radiation1600-57752022-05-0129380781510.1107/S1600577522001874fv5146Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamlineSimone Sala0Yuhe Zhang1Nathaly De La Rosa2Till Dreier3Maik Kahnt4Max Langer5Lars B. Dahlin6Martin Bech7Pablo Villanueva-Perez8Sebastian Kalbfleisch9MAX IV Laboratory, Lund University, 22100 Lund, SwedenDivision of Synchrotron Radiation Research and NanoLund, Department of Physics, Lund University, 22100 Lund, SwedenDepartment of Medical Radiation Physics, Clinical Sciences Lund, Lund University, 22185 Lund, SwedenDepartment of Medical Radiation Physics, Clinical Sciences Lund, Lund University, 22185 Lund, SwedenMAX IV Laboratory, Lund University, 22100 Lund, SwedenUniv Lyon, INSA-Lyon, Université Claude Bernard Lyon 1, UJM-Saint Etienne, CNRS, Inserm, CREATIS UMR 5220, U1206, 69621 Villeurbanne, FranceDepartment of Translational Medicine – Hand Surgery, Lund University, Malmö, SwedenDepartment of Medical Radiation Physics, Clinical Sciences Lund, Lund University, 22185 Lund, SwedenDivision of Synchrotron Radiation Research and NanoLund, Department of Physics, Lund University, 22100 Lund, SwedenMAX IV Laboratory, Lund University, 22100 Lund, SwedenX-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view.http://scripts.iucr.org/cgi-bin/paper?S1600577522001874x-ray microscopyin-line holographyx-ray fluorescence emission spectroscopy |
| spellingShingle | Simone Sala Yuhe Zhang Nathaly De La Rosa Till Dreier Maik Kahnt Max Langer Lars B. Dahlin Martin Bech Pablo Villanueva-Perez Sebastian Kalbfleisch Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline Journal of Synchrotron Radiation x-ray microscopy in-line holography x-ray fluorescence emission spectroscopy |
| title | Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline |
| title_full | Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline |
| title_fullStr | Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline |
| title_full_unstemmed | Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline |
| title_short | Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline |
| title_sort | dose efficient multimodal microscopy of human tissue at a hard x ray nanoprobe beamline |
| topic | x-ray microscopy in-line holography x-ray fluorescence emission spectroscopy |
| url | http://scripts.iucr.org/cgi-bin/paper?S1600577522001874 |
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