Curcumin inhibits the migration of osteoclast precursors and osteoclastogenesis by repressing CCL3 production
Abstract Background Curcumin can inhibit the osteoclastogenesis and the migration of several cells including macrophages. Osteoclast precursors (OCPs) are known to exist as bone marrow-derived macrophages (BMMs). This study aims to explore whether curcumin can prevent the fusion and differentiation...
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-020-03014-2 |
| _version_ | 1828853095845593088 |
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| author | Zhengeng Liang Yan Xue Tao Wang Qi Xie Jiafu Lin Yu Wang |
| author_facet | Zhengeng Liang Yan Xue Tao Wang Qi Xie Jiafu Lin Yu Wang |
| author_sort | Zhengeng Liang |
| collection | DOAJ |
| description | Abstract Background Curcumin can inhibit the osteoclastogenesis and the migration of several cells including macrophages. Osteoclast precursors (OCPs) are known to exist as bone marrow-derived macrophages (BMMs). This study aims to explore whether curcumin can prevent the fusion and differentiation of OCPs to mature osteoclasts by inhibiting OCP migration. Methods In this study, we investigated the role of curcumin in regulating the production of several chemokines (CCL2, CCL3 and CX3CL1) and the migration of OCPs by ELISA, Western blotting and Transwell assays. Furthermore, we explored the role of curcumin in the chemokines-related osteoclastogenesis using pharmacological intervention and virus infection, and used ovariectomized (OVX) mice (osteoporosis model) to explore the effect of curcumin on the production of specific chemokine in vivo. Results The results showed that curcumin significantly reduced the production of CCL3 in OCPs. Moreover, curcumin-inhibited the migration of OCPs was not affected by CCR1 (Receptor of CCL3) overexpression. Remarkably, curcumin-reduced osteoclastogenesis was significantly reversed by CCL3 addition, while CCR1 overexpression did not increase the osteoclastogenesis in the presence of curcumin. Furthermore, in vivo assays also showed that curcumin significantly reduced the production of CCL3 in OCPs in the trabecular bone of OVX mice. Conclusions In conclusion, curcumin prevents the migration of OCPs by reducing CCL3 production, ultimately inhibiting the formation of mature osteoclasts. Therefore, our study provides the clues for improving the clinical strategies of osteoporosis, dental implantation or orthodontic treatment. |
| first_indexed | 2024-12-13T00:06:49Z |
| format | Article |
| id | doaj.art-9f8e927badec46ab9594c56db3f8f39e |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-12-13T00:06:49Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-9f8e927badec46ab9594c56db3f8f39e2022-12-22T00:06:12ZengBMCBMC Complementary Medicine and Therapies2662-76712020-07-012011910.1186/s12906-020-03014-2Curcumin inhibits the migration of osteoclast precursors and osteoclastogenesis by repressing CCL3 productionZhengeng Liang0Yan Xue1Tao Wang2Qi Xie3Jiafu Lin4Yu Wang5Department of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical UniversityDepartment of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical UniversityDepartment of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical UniversityDepartment of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical UniversityFujian Health CollegeDepartment of Orthopaedics, Chifeng Municipal HospitalAbstract Background Curcumin can inhibit the osteoclastogenesis and the migration of several cells including macrophages. Osteoclast precursors (OCPs) are known to exist as bone marrow-derived macrophages (BMMs). This study aims to explore whether curcumin can prevent the fusion and differentiation of OCPs to mature osteoclasts by inhibiting OCP migration. Methods In this study, we investigated the role of curcumin in regulating the production of several chemokines (CCL2, CCL3 and CX3CL1) and the migration of OCPs by ELISA, Western blotting and Transwell assays. Furthermore, we explored the role of curcumin in the chemokines-related osteoclastogenesis using pharmacological intervention and virus infection, and used ovariectomized (OVX) mice (osteoporosis model) to explore the effect of curcumin on the production of specific chemokine in vivo. Results The results showed that curcumin significantly reduced the production of CCL3 in OCPs. Moreover, curcumin-inhibited the migration of OCPs was not affected by CCR1 (Receptor of CCL3) overexpression. Remarkably, curcumin-reduced osteoclastogenesis was significantly reversed by CCL3 addition, while CCR1 overexpression did not increase the osteoclastogenesis in the presence of curcumin. Furthermore, in vivo assays also showed that curcumin significantly reduced the production of CCL3 in OCPs in the trabecular bone of OVX mice. Conclusions In conclusion, curcumin prevents the migration of OCPs by reducing CCL3 production, ultimately inhibiting the formation of mature osteoclasts. Therefore, our study provides the clues for improving the clinical strategies of osteoporosis, dental implantation or orthodontic treatment.http://link.springer.com/article/10.1186/s12906-020-03014-2CurcuminOsteoclastOsteoclast precursorsMigrationCCL3 |
| spellingShingle | Zhengeng Liang Yan Xue Tao Wang Qi Xie Jiafu Lin Yu Wang Curcumin inhibits the migration of osteoclast precursors and osteoclastogenesis by repressing CCL3 production BMC Complementary Medicine and Therapies Curcumin Osteoclast Osteoclast precursors Migration CCL3 |
| title | Curcumin inhibits the migration of osteoclast precursors and osteoclastogenesis by repressing CCL3 production |
| title_full | Curcumin inhibits the migration of osteoclast precursors and osteoclastogenesis by repressing CCL3 production |
| title_fullStr | Curcumin inhibits the migration of osteoclast precursors and osteoclastogenesis by repressing CCL3 production |
| title_full_unstemmed | Curcumin inhibits the migration of osteoclast precursors and osteoclastogenesis by repressing CCL3 production |
| title_short | Curcumin inhibits the migration of osteoclast precursors and osteoclastogenesis by repressing CCL3 production |
| title_sort | curcumin inhibits the migration of osteoclast precursors and osteoclastogenesis by repressing ccl3 production |
| topic | Curcumin Osteoclast Osteoclast precursors Migration CCL3 |
| url | http://link.springer.com/article/10.1186/s12906-020-03014-2 |
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