Phytochemical Composition and α-Glucosidase Inhibitory Activity of Campanumoea lancifolia (Roxb.) Merr Fruit
Hong Guo Shen Guo (HGSG) are the fruits of Campanumoea lancifolia (Roxb.) Merr, which are mainly cultivated in Southwest China. This study was to investigate the ingredients from HGSG extracts and their α-glucosidase inhibition in vitro. The contents of polysaccharide, total flavonoids, total anthoc...
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The editorial department of Science and Technology of Food Industry
2022-08-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021110169 |
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| author | Chunyue HUANG Mengjie MA Lixin NIU Jianjie JIN Man WU Bei TIAN Xiao HU |
| author_facet | Chunyue HUANG Mengjie MA Lixin NIU Jianjie JIN Man WU Bei TIAN Xiao HU |
| author_sort | Chunyue HUANG |
| collection | DOAJ |
| description | Hong Guo Shen Guo (HGSG) are the fruits of Campanumoea lancifolia (Roxb.) Merr, which are mainly cultivated in Southwest China. This study was to investigate the ingredients from HGSG extracts and their α-glucosidase inhibition in vitro. The contents of polysaccharide, total flavonoids, total anthocyanins and total phenols in extracts from HGSG were measured using UV spectrophotometry. Chemical ingredients were further analyzed applying ultra-high performance liquid chromatography-time of flight-tandem mass spectrometry (UPLC-QTOF-MS). The results showed that the content of crude polysaccharide in five batches of HGSG was 27.59%~37.59%. The total flavone content of ethanol extract was 1.22%~1.77%, the total anthocyanin content was 0.90%~1.14%, and the total polyphenol content was 13.11%~18.85%. Twenty one compounds were identified from HGSG, including 10 flavonoids, 3 phenolic acids, 3 organic acids, 3 anthocyanins, 1 amino acid and 1 polyacetylene. The ethanol extract, crude polysaccharide and luteolin of HGSG exhibited potent α-glucosidase inhibitory activities with IC50 values of 7.52, 37.43 and 8.03 μg/mL, respectively, which were significantly higher than that of acarbose (616.17 μg/mL). This study preliminarily defined the material basis of HGSG, and reported for the first time that HGSG had potential anti diabetes activity, which would provide a theoretical reference for the development and utilization of HGSG. |
| first_indexed | 2024-04-13T09:11:32Z |
| format | Article |
| id | doaj.art-9f91e1b6d719426db45ba1fb98d7000d |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-13T09:11:32Z |
| publishDate | 2022-08-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-9f91e1b6d719426db45ba1fb98d7000d2022-12-22T02:52:52ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-08-014316657310.13386/j.issn1002-0306.20211101692021110169-16Phytochemical Composition and α-Glucosidase Inhibitory Activity of Campanumoea lancifolia (Roxb.) Merr FruitChunyue HUANG0Mengjie MA1Lixin NIU2Jianjie JIN3Man WU4Bei TIAN5Xiao HU6China State Institute of Pharmaceutical Industry, Shanghai 201203, ChinaChina State Institute of Pharmaceutical Industry, Shanghai 201203, ChinaChina State Institute of Pharmaceutical Industry, Shanghai 201203, ChinaChina State Institute of Pharmaceutical Industry, Shanghai 201203, ChinaChina State Institute of Pharmaceutical Industry, Shanghai 201203, ChinaGuizhou Innovation Agriculture Development Co., Ltd., Tongren 554300, ChinaChina State Institute of Pharmaceutical Industry, Shanghai 201203, ChinaHong Guo Shen Guo (HGSG) are the fruits of Campanumoea lancifolia (Roxb.) Merr, which are mainly cultivated in Southwest China. This study was to investigate the ingredients from HGSG extracts and their α-glucosidase inhibition in vitro. The contents of polysaccharide, total flavonoids, total anthocyanins and total phenols in extracts from HGSG were measured using UV spectrophotometry. Chemical ingredients were further analyzed applying ultra-high performance liquid chromatography-time of flight-tandem mass spectrometry (UPLC-QTOF-MS). The results showed that the content of crude polysaccharide in five batches of HGSG was 27.59%~37.59%. The total flavone content of ethanol extract was 1.22%~1.77%, the total anthocyanin content was 0.90%~1.14%, and the total polyphenol content was 13.11%~18.85%. Twenty one compounds were identified from HGSG, including 10 flavonoids, 3 phenolic acids, 3 organic acids, 3 anthocyanins, 1 amino acid and 1 polyacetylene. The ethanol extract, crude polysaccharide and luteolin of HGSG exhibited potent α-glucosidase inhibitory activities with IC50 values of 7.52, 37.43 and 8.03 μg/mL, respectively, which were significantly higher than that of acarbose (616.17 μg/mL). This study preliminarily defined the material basis of HGSG, and reported for the first time that HGSG had potential anti diabetes activity, which would provide a theoretical reference for the development and utilization of HGSG.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021110169campanumoea lancifolia (roxb.) merr fruitsultra-high performance liquid chromatography-time of flight-tandem mass spectrometrychemical ingredientsα-glucosidase inhibitory activity |
| spellingShingle | Chunyue HUANG Mengjie MA Lixin NIU Jianjie JIN Man WU Bei TIAN Xiao HU Phytochemical Composition and α-Glucosidase Inhibitory Activity of Campanumoea lancifolia (Roxb.) Merr Fruit Shipin gongye ke-ji campanumoea lancifolia (roxb.) merr fruits ultra-high performance liquid chromatography-time of flight-tandem mass spectrometry chemical ingredients α-glucosidase inhibitory activity |
| title | Phytochemical Composition and α-Glucosidase Inhibitory Activity of Campanumoea lancifolia (Roxb.) Merr Fruit |
| title_full | Phytochemical Composition and α-Glucosidase Inhibitory Activity of Campanumoea lancifolia (Roxb.) Merr Fruit |
| title_fullStr | Phytochemical Composition and α-Glucosidase Inhibitory Activity of Campanumoea lancifolia (Roxb.) Merr Fruit |
| title_full_unstemmed | Phytochemical Composition and α-Glucosidase Inhibitory Activity of Campanumoea lancifolia (Roxb.) Merr Fruit |
| title_short | Phytochemical Composition and α-Glucosidase Inhibitory Activity of Campanumoea lancifolia (Roxb.) Merr Fruit |
| title_sort | phytochemical composition and α glucosidase inhibitory activity of campanumoea lancifolia roxb merr fruit |
| topic | campanumoea lancifolia (roxb.) merr fruits ultra-high performance liquid chromatography-time of flight-tandem mass spectrometry chemical ingredients α-glucosidase inhibitory activity |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021110169 |
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