Acinetobacter baumannii extracted silver nanoparticles: A study on the characterization and antibacterial activity
Background: Antibiotic resistance is a worldwide problem, associated with increased mortality. Biosynthesized silver nanoparticles are promising solution. Aim: To improve the anti-microbial resistance using Acinetobacter baumannii (A. baumannii) biosynthesized silver nitrate.Methods: Optimization o...
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| Format: | Article |
| Language: | English |
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Zagazig University, Faculty of Medicine
2022-02-01
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| Series: | Microbes and Infectious Diseases |
| Subjects: | |
| Online Access: | https://mid.journals.ekb.eg/article_211483_ff16dbdd0b8fd70a27becf30f529c774.pdf |
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| author | Mona Abdelhamid Rateb Abass Magdy Khalil A. B. Mohammed fatma khalil |
| author_facet | Mona Abdelhamid Rateb Abass Magdy Khalil A. B. Mohammed fatma khalil |
| author_sort | Mona Abdelhamid |
| collection | DOAJ |
| description | Background: Antibiotic resistance is a worldwide problem, associated with increased mortality. Biosynthesized silver nanoparticles are promising solution. Aim: To improve the anti-microbial resistance using Acinetobacter baumannii (A. baumannii) biosynthesized silver nitrate.Methods: Optimization of biosynthesis condition and characterization by Ultraviolet-Visible spectroscopy (U-V-Vis) at a wavelength from 300 to 400 nm, ±1.67 nm. The Acinetobacter baumannii biosynthesized silver nitrate was examined using transmission electron microscope (TEM). Fourier transformed infrared spectroscopy was done to study the capping material, X-ray spectroscopy confirm silver nanoparticles synthesis with particle size match TEM measuring and testing its antibacterial effect was done. Results: The obtained U.V Spectroscopy and TEM characters of silver nitrate biosynthesized A.baumannii. Acinetobacter baumannii biosynthesized silver nitrate was obtained indicated. Minimum inhibitory concentration (MIC) was from 1-3 mg/ml against extreme drug resistance bacteria. the size of silver nanoparticles was found to be between 18 nm and 50 nm. The AgNPs were found to be stable for over 6 months at 37°C.The MIC for AgNPs formed from A. baumannii CFF was detected as 2.85 mg/ml, MBC (minimal bactericidal concentration) was 3 mg/ml for K. pneumonia, MIC was 1 mg/ml, MBC was 0.5 mg/ml for P. aeruginosa and MIC was 1.5 mg/ml and MBC was 1 mg/ml for A. baumannii isolates. Lethal concentration (LC) 50% was 60.584 ug/ml) assessed by Sulforhodamine-B assay on hepatocellular carcinoma cell line. Conclusion: Acinetobacter baumannii extracted silver nitrate has A promising antibacterial action that may help in solving the multidrug resistance bacteria. |
| first_indexed | 2024-04-11T09:15:10Z |
| format | Article |
| id | doaj.art-9f932e85573941deb96fdde53130ca0c |
| institution | Directory Open Access Journal |
| issn | 2682-4132 2682-4140 |
| language | English |
| last_indexed | 2024-04-11T09:15:10Z |
| publishDate | 2022-02-01 |
| publisher | Zagazig University, Faculty of Medicine |
| record_format | Article |
| series | Microbes and Infectious Diseases |
| spelling | doaj.art-9f932e85573941deb96fdde53130ca0c2022-12-22T04:32:24ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402022-02-013113514810.21608/mid.2021.109802.1212211483Acinetobacter baumannii extracted silver nanoparticles: A study on the characterization and antibacterial activityMona Abdelhamid0Rateb Abass1Magdy Khalil2A. B. Mohammed3fatma khalil4Clinical pharmacist. National Liver Institute, Menoufia UniversityAssociate Professor of Microbial Genetics, Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, EgyptProfessor of Anesthesia and Intensive Care, National Liver Institute, Menoufia University, Shebin El-kom 32511, EgyptLecturer of Microbial Biotechnology, Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, EgyptMicrobiology nd Immunology, National Liver Institute, Menoufia University, Shebin El-kom, EgyptBackground: Antibiotic resistance is a worldwide problem, associated with increased mortality. Biosynthesized silver nanoparticles are promising solution. Aim: To improve the anti-microbial resistance using Acinetobacter baumannii (A. baumannii) biosynthesized silver nitrate.Methods: Optimization of biosynthesis condition and characterization by Ultraviolet-Visible spectroscopy (U-V-Vis) at a wavelength from 300 to 400 nm, ±1.67 nm. The Acinetobacter baumannii biosynthesized silver nitrate was examined using transmission electron microscope (TEM). Fourier transformed infrared spectroscopy was done to study the capping material, X-ray spectroscopy confirm silver nanoparticles synthesis with particle size match TEM measuring and testing its antibacterial effect was done. Results: The obtained U.V Spectroscopy and TEM characters of silver nitrate biosynthesized A.baumannii. Acinetobacter baumannii biosynthesized silver nitrate was obtained indicated. Minimum inhibitory concentration (MIC) was from 1-3 mg/ml against extreme drug resistance bacteria. the size of silver nanoparticles was found to be between 18 nm and 50 nm. The AgNPs were found to be stable for over 6 months at 37°C.The MIC for AgNPs formed from A. baumannii CFF was detected as 2.85 mg/ml, MBC (minimal bactericidal concentration) was 3 mg/ml for K. pneumonia, MIC was 1 mg/ml, MBC was 0.5 mg/ml for P. aeruginosa and MIC was 1.5 mg/ml and MBC was 1 mg/ml for A. baumannii isolates. Lethal concentration (LC) 50% was 60.584 ug/ml) assessed by Sulforhodamine-B assay on hepatocellular carcinoma cell line. Conclusion: Acinetobacter baumannii extracted silver nitrate has A promising antibacterial action that may help in solving the multidrug resistance bacteria.https://mid.journals.ekb.eg/article_211483_ff16dbdd0b8fd70a27becf30f529c774.pdfsilver nanoparticlesantibiotic resistanceacinetobacter baumanniicytotoxicityhepatocellular carcinoma |
| spellingShingle | Mona Abdelhamid Rateb Abass Magdy Khalil A. B. Mohammed fatma khalil Acinetobacter baumannii extracted silver nanoparticles: A study on the characterization and antibacterial activity Microbes and Infectious Diseases silver nanoparticles antibiotic resistance acinetobacter baumannii cytotoxicity hepatocellular carcinoma |
| title | Acinetobacter baumannii extracted silver nanoparticles: A study on the characterization and antibacterial activity |
| title_full | Acinetobacter baumannii extracted silver nanoparticles: A study on the characterization and antibacterial activity |
| title_fullStr | Acinetobacter baumannii extracted silver nanoparticles: A study on the characterization and antibacterial activity |
| title_full_unstemmed | Acinetobacter baumannii extracted silver nanoparticles: A study on the characterization and antibacterial activity |
| title_short | Acinetobacter baumannii extracted silver nanoparticles: A study on the characterization and antibacterial activity |
| title_sort | acinetobacter baumannii extracted silver nanoparticles a study on the characterization and antibacterial activity |
| topic | silver nanoparticles antibiotic resistance acinetobacter baumannii cytotoxicity hepatocellular carcinoma |
| url | https://mid.journals.ekb.eg/article_211483_ff16dbdd0b8fd70a27becf30f529c774.pdf |
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