Occurrence of blaOXA-23 gene in imipenem-susceptible Acinetobacter baumannii
The aim of the current study was to describe the occurrence of the blaOXA-23 gene and the ISAba1 element in imipenem-susceptible Acinetobacter baumannii strains. By performing the polymerase chain reaction mapping using combinations of ISAba1 forward primers and the blaOXA-23-like gene reverse prime...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2011-06-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400020&lng=en&tlng=en |
| _version_ | 1797718460423208960 |
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| author | Karyne Rangel Carvalho Ana Paula D'Alincourt Carvalho-Assef Lia Galvão dos Santos Maria José Félix Pereira Marise Dutra Asensi |
| author_facet | Karyne Rangel Carvalho Ana Paula D'Alincourt Carvalho-Assef Lia Galvão dos Santos Maria José Félix Pereira Marise Dutra Asensi |
| author_sort | Karyne Rangel Carvalho |
| collection | DOAJ |
| description | The aim of the current study was to describe the occurrence of the blaOXA-23 gene and the ISAba1 element in imipenem-susceptible Acinetobacter baumannii strains. By performing the polymerase chain reaction mapping using combinations of ISAba1 forward primers and the blaOXA-23-like gene reverse primers, we demonstrated that the ISAba1 element did not occur upstream of the blaOXA-23 gene in five of 31 isolates, which explained the lack of resistance to imipenem despite the presence of the blaOXA-23 gene. All of the blaOXA-23-positive isolates were susceptible to imipenem and meropenem with minimal inhibitory concentration < 4 µg/mL. Pulsed-field gel electrophoresis analysis revealed four genotypes among the five blaOXA-23-positive isolates. The current report of the blaOXA-23 gene in imipenem-susceptible isolates provided evidence that this gene may be silently spread in a hospital environment and highlighted the threat of undetected reservoirs of carbapenemase genes. |
| first_indexed | 2024-03-12T08:52:06Z |
| format | Article |
| id | doaj.art-9f9970969d8e43a2a8a5d01667ece0be |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:52:06Z |
| publishDate | 2011-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-9f9970969d8e43a2a8a5d01667ece0be2023-09-02T16:17:54ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602011-06-01106450550610.1590/S0074-02762011000400020S0074-02762011000400020Occurrence of blaOXA-23 gene in imipenem-susceptible Acinetobacter baumanniiKaryne Rangel Carvalho0Ana Paula D'Alincourt Carvalho-Assef1Lia Galvão dos Santos2Maria José Félix Pereira3Marise Dutra Asensi4Fundação Oswaldo CruzFundação Oswaldo CruzHospital Federal de BonsucessoRede Labs D'OrFundação Oswaldo CruzThe aim of the current study was to describe the occurrence of the blaOXA-23 gene and the ISAba1 element in imipenem-susceptible Acinetobacter baumannii strains. By performing the polymerase chain reaction mapping using combinations of ISAba1 forward primers and the blaOXA-23-like gene reverse primers, we demonstrated that the ISAba1 element did not occur upstream of the blaOXA-23 gene in five of 31 isolates, which explained the lack of resistance to imipenem despite the presence of the blaOXA-23 gene. All of the blaOXA-23-positive isolates were susceptible to imipenem and meropenem with minimal inhibitory concentration < 4 µg/mL. Pulsed-field gel electrophoresis analysis revealed four genotypes among the five blaOXA-23-positive isolates. The current report of the blaOXA-23 gene in imipenem-susceptible isolates provided evidence that this gene may be silently spread in a hospital environment and highlighted the threat of undetected reservoirs of carbapenemase genes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400020&lng=en&tlng=enimipenem-susceptibleAcinetobacter baumanniiblaOXA-23 |
| spellingShingle | Karyne Rangel Carvalho Ana Paula D'Alincourt Carvalho-Assef Lia Galvão dos Santos Maria José Félix Pereira Marise Dutra Asensi Occurrence of blaOXA-23 gene in imipenem-susceptible Acinetobacter baumannii Memorias do Instituto Oswaldo Cruz imipenem-susceptible Acinetobacter baumannii blaOXA-23 |
| title | Occurrence of blaOXA-23 gene in imipenem-susceptible Acinetobacter baumannii |
| title_full | Occurrence of blaOXA-23 gene in imipenem-susceptible Acinetobacter baumannii |
| title_fullStr | Occurrence of blaOXA-23 gene in imipenem-susceptible Acinetobacter baumannii |
| title_full_unstemmed | Occurrence of blaOXA-23 gene in imipenem-susceptible Acinetobacter baumannii |
| title_short | Occurrence of blaOXA-23 gene in imipenem-susceptible Acinetobacter baumannii |
| title_sort | occurrence of blaoxa 23 gene in imipenem susceptible acinetobacter baumannii |
| topic | imipenem-susceptible Acinetobacter baumannii blaOXA-23 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400020&lng=en&tlng=en |
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