Virulence Pattern Analysis of Three <i>Listeria monocytogenes</i> Lineage I Epidemic Strains with Distinct Outbreak Histories
Strains of the food-borne pathogen <i>Listeria</i> (<i>L</i>.) <i>monocytogenes</i> have diverse virulence potential. This study focused on the virulence of three outbreak strains: the CC1 strain PF49 (serovar 4b) from a cheese-associated outbreak in Switzerland,...
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MDPI AG
2021-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/8/1745 |
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| author | Martin Wagner Jörg Slaghuis Werner Göbel José Antonio Vázquez-Boland Kathrin Rychli Stephan Schmitz-Esser |
| author_facet | Martin Wagner Jörg Slaghuis Werner Göbel José Antonio Vázquez-Boland Kathrin Rychli Stephan Schmitz-Esser |
| author_sort | Martin Wagner |
| collection | DOAJ |
| description | Strains of the food-borne pathogen <i>Listeria</i> (<i>L</i>.) <i>monocytogenes</i> have diverse virulence potential. This study focused on the virulence of three outbreak strains: the CC1 strain PF49 (serovar 4b) from a cheese-associated outbreak in Switzerland, the clinical CC2 strain F80594 (serovar 4b), and strain G6006 (CC3, serovar 1/2a), responsible for a large gastroenteritis outbreak in the USA due to chocolate milk. We analysed the genomes and characterized the virulence in vitro and in vivo. Whole-genome sequencing revealed a high conservation of the major virulence genes. Minor deviations of the gene contents were found in the autolysins Ami, Auto, and IspC. Moreover, different ActA variants were present. Strain PF49 and F80594 showed prolonged survival in the liver of infected mice. Invasion and intracellular proliferation were similar for all strains, but the CC1 and CC2 strains showed increased spreading in intestinal epithelial Caco2 cells compared to strain G6006. Overall, this study revealed long-term survival of serovar 4b strains F80594 and PF49 in the liver of mice. Future work will be needed to determine the genes and molecular mechanism behind the long-term survival of <i>L. monocytogenes</i> strains in organs. |
| first_indexed | 2024-03-10T08:34:24Z |
| format | Article |
| id | doaj.art-9f9a5f25cdd44c998ede14e44d075743 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T08:34:24Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-9f9a5f25cdd44c998ede14e44d0757432023-11-22T08:48:15ZengMDPI AGMicroorganisms2076-26072021-08-0198174510.3390/microorganisms9081745Virulence Pattern Analysis of Three <i>Listeria monocytogenes</i> Lineage I Epidemic Strains with Distinct Outbreak HistoriesMartin Wagner0Jörg Slaghuis1Werner Göbel2José Antonio Vázquez-Boland3Kathrin Rychli4Stephan Schmitz-Esser5Unit for Food Microbiology, Institute for Food Safety, Food Technology and Veterinary Public Health, University of Veterinary Medicine, 1210 Vienna, AustriaMicrobiological Analytics, Merck KGaA, Frankfurterstrasse 250, 64293 Darmstadt, GermanyCair of Microbiology, University of Würzburg, Am Hubland 1, 97074 Würzburg, GermanyMicrobial Pathogenesis Laboratory, Edinburgh Medical School (Biomedical Sciences-Infection Medicine), University of Edinburgh, Edinburgh EH16 4SB, UKUnit for Food Microbiology, Institute for Food Safety, Food Technology and Veterinary Public Health, University of Veterinary Medicine, 1210 Vienna, AustriaDepartment of Animal Science, Iowa State University, Ames, IA 50011, USAStrains of the food-borne pathogen <i>Listeria</i> (<i>L</i>.) <i>monocytogenes</i> have diverse virulence potential. This study focused on the virulence of three outbreak strains: the CC1 strain PF49 (serovar 4b) from a cheese-associated outbreak in Switzerland, the clinical CC2 strain F80594 (serovar 4b), and strain G6006 (CC3, serovar 1/2a), responsible for a large gastroenteritis outbreak in the USA due to chocolate milk. We analysed the genomes and characterized the virulence in vitro and in vivo. Whole-genome sequencing revealed a high conservation of the major virulence genes. Minor deviations of the gene contents were found in the autolysins Ami, Auto, and IspC. Moreover, different ActA variants were present. Strain PF49 and F80594 showed prolonged survival in the liver of infected mice. Invasion and intracellular proliferation were similar for all strains, but the CC1 and CC2 strains showed increased spreading in intestinal epithelial Caco2 cells compared to strain G6006. Overall, this study revealed long-term survival of serovar 4b strains F80594 and PF49 in the liver of mice. Future work will be needed to determine the genes and molecular mechanism behind the long-term survival of <i>L. monocytogenes</i> strains in organs.https://www.mdpi.com/2076-2607/9/8/1745pathogenicitywhole-genome analysisprolonged survival |
| spellingShingle | Martin Wagner Jörg Slaghuis Werner Göbel José Antonio Vázquez-Boland Kathrin Rychli Stephan Schmitz-Esser Virulence Pattern Analysis of Three <i>Listeria monocytogenes</i> Lineage I Epidemic Strains with Distinct Outbreak Histories Microorganisms pathogenicity whole-genome analysis prolonged survival |
| title | Virulence Pattern Analysis of Three <i>Listeria monocytogenes</i> Lineage I Epidemic Strains with Distinct Outbreak Histories |
| title_full | Virulence Pattern Analysis of Three <i>Listeria monocytogenes</i> Lineage I Epidemic Strains with Distinct Outbreak Histories |
| title_fullStr | Virulence Pattern Analysis of Three <i>Listeria monocytogenes</i> Lineage I Epidemic Strains with Distinct Outbreak Histories |
| title_full_unstemmed | Virulence Pattern Analysis of Three <i>Listeria monocytogenes</i> Lineage I Epidemic Strains with Distinct Outbreak Histories |
| title_short | Virulence Pattern Analysis of Three <i>Listeria monocytogenes</i> Lineage I Epidemic Strains with Distinct Outbreak Histories |
| title_sort | virulence pattern analysis of three i listeria monocytogenes i lineage i epidemic strains with distinct outbreak histories |
| topic | pathogenicity whole-genome analysis prolonged survival |
| url | https://www.mdpi.com/2076-2607/9/8/1745 |
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