Analysis of mitochondrial respiration and ATP synthase in frozen brain tissues
Studying mitochondrial respiration capacity is essential for gaining insights into mitochondrial functions. In frozen tissue samples, however, our ability to study mitochondrial respiration is restricted by damage elicited to the inner mitochondrial membranes by freeze-thaw cycles. We developed an a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023010952 |
| _version_ | 1797851926879010816 |
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| author | Pamela J. Yao Rachel Munk Myriam Gorospe Dimitrios Kapogiannis |
| author_facet | Pamela J. Yao Rachel Munk Myriam Gorospe Dimitrios Kapogiannis |
| author_sort | Pamela J. Yao |
| collection | DOAJ |
| description | Studying mitochondrial respiration capacity is essential for gaining insights into mitochondrial functions. In frozen tissue samples, however, our ability to study mitochondrial respiration is restricted by damage elicited to the inner mitochondrial membranes by freeze-thaw cycles. We developed an approach that combines multiple assays and is tailored towards assessing mitochondrial electron transport chain and ATP synthase in frozen tissues. Using small amounts of frozen tissue, we systematically analyzed the quantity as well as activity of both the electron transport chain complexes and ATP synthase in rat brains during postnatal development. We reveal a previously little-known pattern of increasing mitochondrial respiration capacity with brain development. In addition to providing proof-of-principle evidence that mitochondrial activity changes during brain development, our study details an approach that can be applicable to many other types of frozen cell or tissue samples. |
| first_indexed | 2024-04-09T19:24:43Z |
| format | Article |
| id | doaj.art-9f9c3b847c6a419d95a16ec866021a67 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-09T19:24:43Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-9f9c3b847c6a419d95a16ec866021a672023-04-05T08:18:35ZengElsevierHeliyon2405-84402023-03-0193e13888Analysis of mitochondrial respiration and ATP synthase in frozen brain tissuesPamela J. Yao0Rachel Munk1Myriam Gorospe2Dimitrios Kapogiannis3Laboratory of Clinical Investigation, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, MD, USA; Corresponding author. Laboratory of Clinical Investigation, NIA/NIH Biomedical Research Center, Baltimore, MD 21224, USA.Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, MD, USALaboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, MD, USALaboratory of Clinical Investigation, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, MD, USA; Corresponding author. Laboratory of Clinical Investigation, NIA/NIH Biomedical Research Center, Baltimore, MD 21224, USA.Studying mitochondrial respiration capacity is essential for gaining insights into mitochondrial functions. In frozen tissue samples, however, our ability to study mitochondrial respiration is restricted by damage elicited to the inner mitochondrial membranes by freeze-thaw cycles. We developed an approach that combines multiple assays and is tailored towards assessing mitochondrial electron transport chain and ATP synthase in frozen tissues. Using small amounts of frozen tissue, we systematically analyzed the quantity as well as activity of both the electron transport chain complexes and ATP synthase in rat brains during postnatal development. We reveal a previously little-known pattern of increasing mitochondrial respiration capacity with brain development. In addition to providing proof-of-principle evidence that mitochondrial activity changes during brain development, our study details an approach that can be applicable to many other types of frozen cell or tissue samples.http://www.sciencedirect.com/science/article/pii/S2405844023010952Mitochondrial respirationFrozen samplesBrainDevelopmentElectron transport chainATP synthase |
| spellingShingle | Pamela J. Yao Rachel Munk Myriam Gorospe Dimitrios Kapogiannis Analysis of mitochondrial respiration and ATP synthase in frozen brain tissues Heliyon Mitochondrial respiration Frozen samples Brain Development Electron transport chain ATP synthase |
| title | Analysis of mitochondrial respiration and ATP synthase in frozen brain tissues |
| title_full | Analysis of mitochondrial respiration and ATP synthase in frozen brain tissues |
| title_fullStr | Analysis of mitochondrial respiration and ATP synthase in frozen brain tissues |
| title_full_unstemmed | Analysis of mitochondrial respiration and ATP synthase in frozen brain tissues |
| title_short | Analysis of mitochondrial respiration and ATP synthase in frozen brain tissues |
| title_sort | analysis of mitochondrial respiration and atp synthase in frozen brain tissues |
| topic | Mitochondrial respiration Frozen samples Brain Development Electron transport chain ATP synthase |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023010952 |
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