Extração de esterase de fígado suíno (PLE) Pig liver esterase (PLE) extraction
<abstract language="eng">A simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/conce...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2006-07-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000400040 |
| _version_ | 1818700243299991552 |
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| author | Henrique Celso Trevisan João Batista de Medeiros Helen Cristina Fávero Lisboa |
| author_facet | Henrique Celso Trevisan João Batista de Medeiros Helen Cristina Fávero Lisboa |
| author_sort | Henrique Celso Trevisan |
| collection | DOAJ |
| description | <abstract language="eng">A simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 kU of PLE suspension were obtained after 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thus obtained was stable, showing 96-100% of the initial activity after 7 months in refrigerator at 4°C. |
| first_indexed | 2024-12-17T15:01:51Z |
| format | Article |
| id | doaj.art-9fa315413e5b48d08ee08a3843fdbff4 |
| institution | Directory Open Access Journal |
| issn | 0100-4042 1678-7064 |
| language | English |
| last_indexed | 2024-12-17T15:01:51Z |
| publishDate | 2006-07-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-9fa315413e5b48d08ee08a3843fdbff42022-12-21T21:43:52ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642006-07-0129486586710.1590/S0100-40422006000400040Extração de esterase de fígado suíno (PLE) Pig liver esterase (PLE) extractionHenrique Celso TrevisanJoão Batista de MedeirosHelen Cristina Fávero Lisboa<abstract language="eng">A simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 kU of PLE suspension were obtained after 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thus obtained was stable, showing 96-100% of the initial activity after 7 months in refrigerator at 4°C.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000400040porcine liver esterasePLEbiocatalysis |
| spellingShingle | Henrique Celso Trevisan João Batista de Medeiros Helen Cristina Fávero Lisboa Extração de esterase de fígado suíno (PLE) Pig liver esterase (PLE) extraction Química Nova porcine liver esterase PLE biocatalysis |
| title | Extração de esterase de fígado suíno (PLE) Pig liver esterase (PLE) extraction |
| title_full | Extração de esterase de fígado suíno (PLE) Pig liver esterase (PLE) extraction |
| title_fullStr | Extração de esterase de fígado suíno (PLE) Pig liver esterase (PLE) extraction |
| title_full_unstemmed | Extração de esterase de fígado suíno (PLE) Pig liver esterase (PLE) extraction |
| title_short | Extração de esterase de fígado suíno (PLE) Pig liver esterase (PLE) extraction |
| title_sort | extracao de esterase de figado suino ple pig liver esterase ple extraction |
| topic | porcine liver esterase PLE biocatalysis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000400040 |
| work_keys_str_mv | AT henriquecelsotrevisan extracaodeesterasedefigadosuinoplepigliveresterasepleextraction AT joaobatistademedeiros extracaodeesterasedefigadosuinoplepigliveresterasepleextraction AT helencristinafaverolisboa extracaodeesterasedefigadosuinoplepigliveresterasepleextraction |