Gene expression in human hippocampus from cocaine abusers identifies genes which regulate extracellular matrix remodeling.
The chronic effects of cocaine abuse on brain structure and function are blamed for the inability of most addicts to remain abstinent. Part of the difficulty in preventing relapse is the persisting memory of the intense euphoria or cocaine "rush". Most abused drugs and alcohol induce neuro...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2007-11-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2063513?pdf=render |
| _version_ | 1819070844471607296 |
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| author | Deborah C Mash Jarlath ffrench-Mullen Nikhil Adi Yujing Qin Andrew Buck John Pablo |
| author_facet | Deborah C Mash Jarlath ffrench-Mullen Nikhil Adi Yujing Qin Andrew Buck John Pablo |
| author_sort | Deborah C Mash |
| collection | DOAJ |
| description | The chronic effects of cocaine abuse on brain structure and function are blamed for the inability of most addicts to remain abstinent. Part of the difficulty in preventing relapse is the persisting memory of the intense euphoria or cocaine "rush". Most abused drugs and alcohol induce neuroplastic changes in brain pathways subserving emotion and cognition. Such changes may account for the consolidation and structural reconfiguration of synaptic connections with exposure to cocaine. Adaptive hippocampal plasticity could be related to specific patterns of gene expression with chronic cocaine abuse. Here, we compare gene expression profiles in the human hippocampus from cocaine addicts and age-matched drug-free control subjects. Cocaine abusers had 151 gene transcripts upregulated, while 91 gene transcripts were downregulated. Topping the list of cocaine-regulated transcripts was RECK in the human hippocampus (FC = 2.0; p<0.05). RECK is a membrane-anchored MMP inhibitor that is implicated in the coordinated regulation of extracellular matrix integrity and angiogenesis. In keeping with elevated RECK expression, active MMP9 protein levels were decreased in the hippocampus from cocaine abusers. Pathway analysis identified other genes regulated by cocaine that code for proteins involved in the remodeling of the cytomatrix and synaptic connections and the inhibition of blood vessel proliferation (PCDH8, LAMB1, ITGB6, CTGF and EphB4). The observed microarray phenotype in the human hippocampus identified RECK and other region-specific genes that may promote long-lasting structural changes with repeated cocaine abuse. Extracellular matrix remodeling in the hippocampus may be a persisting effect of chronic abuse that contributes to the compulsive and relapsing nature of cocaine addiction. |
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| format | Article |
| id | doaj.art-9fa5ffb72f9945f9b59bf40eb16bcd14 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T17:12:24Z |
| publishDate | 2007-11-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-9fa5ffb72f9945f9b59bf40eb16bcd142022-12-21T18:56:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032007-11-01211e118710.1371/journal.pone.0001187Gene expression in human hippocampus from cocaine abusers identifies genes which regulate extracellular matrix remodeling.Deborah C MashJarlath ffrench-MullenNikhil AdiYujing QinAndrew BuckJohn PabloThe chronic effects of cocaine abuse on brain structure and function are blamed for the inability of most addicts to remain abstinent. Part of the difficulty in preventing relapse is the persisting memory of the intense euphoria or cocaine "rush". Most abused drugs and alcohol induce neuroplastic changes in brain pathways subserving emotion and cognition. Such changes may account for the consolidation and structural reconfiguration of synaptic connections with exposure to cocaine. Adaptive hippocampal plasticity could be related to specific patterns of gene expression with chronic cocaine abuse. Here, we compare gene expression profiles in the human hippocampus from cocaine addicts and age-matched drug-free control subjects. Cocaine abusers had 151 gene transcripts upregulated, while 91 gene transcripts were downregulated. Topping the list of cocaine-regulated transcripts was RECK in the human hippocampus (FC = 2.0; p<0.05). RECK is a membrane-anchored MMP inhibitor that is implicated in the coordinated regulation of extracellular matrix integrity and angiogenesis. In keeping with elevated RECK expression, active MMP9 protein levels were decreased in the hippocampus from cocaine abusers. Pathway analysis identified other genes regulated by cocaine that code for proteins involved in the remodeling of the cytomatrix and synaptic connections and the inhibition of blood vessel proliferation (PCDH8, LAMB1, ITGB6, CTGF and EphB4). The observed microarray phenotype in the human hippocampus identified RECK and other region-specific genes that may promote long-lasting structural changes with repeated cocaine abuse. Extracellular matrix remodeling in the hippocampus may be a persisting effect of chronic abuse that contributes to the compulsive and relapsing nature of cocaine addiction.http://europepmc.org/articles/PMC2063513?pdf=render |
| spellingShingle | Deborah C Mash Jarlath ffrench-Mullen Nikhil Adi Yujing Qin Andrew Buck John Pablo Gene expression in human hippocampus from cocaine abusers identifies genes which regulate extracellular matrix remodeling. PLoS ONE |
| title | Gene expression in human hippocampus from cocaine abusers identifies genes which regulate extracellular matrix remodeling. |
| title_full | Gene expression in human hippocampus from cocaine abusers identifies genes which regulate extracellular matrix remodeling. |
| title_fullStr | Gene expression in human hippocampus from cocaine abusers identifies genes which regulate extracellular matrix remodeling. |
| title_full_unstemmed | Gene expression in human hippocampus from cocaine abusers identifies genes which regulate extracellular matrix remodeling. |
| title_short | Gene expression in human hippocampus from cocaine abusers identifies genes which regulate extracellular matrix remodeling. |
| title_sort | gene expression in human hippocampus from cocaine abusers identifies genes which regulate extracellular matrix remodeling |
| url | http://europepmc.org/articles/PMC2063513?pdf=render |
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