Molecular epidemiology and phylogenetic analysis of human T-lymphotropic virus type 1 in the tax gene and it association with adult t-cell leukemia/lymphoma disorders
Background and Objectives: Human T-lymphotropic virus type-1 (HTLV-1) belongs to retrovirus family that causes the neurological disorder HTLV-1 adult T-cell leukemia/lymphoma (ATLL). Since 1980, seven subtypes of the virus have been recognized. HTLV-1 is prevalent and endemic in some regions, such a...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2021-08-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/3051 |
| _version_ | 1798028830095441920 |
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| author | Samira Pourrezaei Shahrzad Shadabi Maryam Gheidishahran Abbas Rahimiforoushani Masoume Akhbari Mahnaz Tavakoli Mahshid Safavi Mobina Madihi Mehdi Norouzi |
| author_facet | Samira Pourrezaei Shahrzad Shadabi Maryam Gheidishahran Abbas Rahimiforoushani Masoume Akhbari Mahnaz Tavakoli Mahshid Safavi Mobina Madihi Mehdi Norouzi |
| author_sort | Samira Pourrezaei |
| collection | DOAJ |
| description | Background and Objectives: Human T-lymphotropic virus type-1 (HTLV-1) belongs to retrovirus family that causes the neurological disorder HTLV-1 adult T-cell leukemia/lymphoma (ATLL). Since 1980, seven subtypes of the virus have been recognized. HTLV-1 is prevalent and endemic in some regions, such as Africa, Japan, South America and Iran as the endemic regions of the HTLV-1 in the Middle East. To study HTLV-1 subtypes and routes of virus spread in Iran, phylogenetic and phylodynamic analyses were performed and for as much as no previous phylogenetic studies were conducted in Tehran, we do this survey. To this purpose, the Tax region of HTLV-1 was used.
Materials and Methods: In this study 100 samples were collected from blood donors in Tehran. All samples were screened for anti-HTLV-I antibodies by ELISA. Then, genomic DNA was extracted from all positive samples (10 people), and for confirmation of infection, ordinary PCR was performed for both the HBZ and LTR regions. Moreover, the Tax region was amplified and purified PCR products were sequenced and analyzed, and finally, a phylogenetic tree was constructed using Mega X software.
Results: Phylogenetic analysis confirmed that isolates from Iran, Japan, Brazil, and Africa are located within the extensive ‘‘transcontinental’’ subgroup A clade of HTLV-1 Cosmopolitan subtype a. The Japanese sequences are the closest to the Iranian sequences and have the most genetic similarity with them.
Conclusion: Through phylogenetic and phylodynamic analyses HTLV-1 strain in Tehran were characterized in Iran. The appearance of HTLV-1 in Iran was probably happened by the ancient Silk Road which linked China to Antioch. |
| first_indexed | 2024-04-11T19:15:46Z |
| format | Article |
| id | doaj.art-9fa641a4e6ee42b6b88611838d967cf0 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-04-11T19:15:46Z |
| publishDate | 2021-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-9fa641a4e6ee42b6b88611838d967cf02022-12-22T04:07:27ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472021-08-0113410.18502/ijm.v13i4.6976Molecular epidemiology and phylogenetic analysis of human T-lymphotropic virus type 1 in the tax gene and it association with adult t-cell leukemia/lymphoma disordersSamira Pourrezaei0Shahrzad Shadabi1Maryam Gheidishahran2Abbas Rahimiforoushani3Masoume Akhbari4Mahnaz Tavakoli5Mahshid Safavi6Mobina Madihi7Mehdi Norouzi8Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Virology, School of Medical Sciences, Kerman University of Medical Sciences, Kerman, IranDepartment of Medical Hematology and Blood Transfusion, School of Allied Medical Science, Iran University of Medical Sciences, Tehran, IranDepartment of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Molecular Medicine, School of Medical Science, Karaj University of Medical Sciences, Karaj, IranDepartment of Medical Microbiology, School of Medical Science, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Medical Microbiology, School of Medical Science, Karaj University of Medical Sciences, IranDepartment of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Virology, Institute of Health Research, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, IranBackground and Objectives: Human T-lymphotropic virus type-1 (HTLV-1) belongs to retrovirus family that causes the neurological disorder HTLV-1 adult T-cell leukemia/lymphoma (ATLL). Since 1980, seven subtypes of the virus have been recognized. HTLV-1 is prevalent and endemic in some regions, such as Africa, Japan, South America and Iran as the endemic regions of the HTLV-1 in the Middle East. To study HTLV-1 subtypes and routes of virus spread in Iran, phylogenetic and phylodynamic analyses were performed and for as much as no previous phylogenetic studies were conducted in Tehran, we do this survey. To this purpose, the Tax region of HTLV-1 was used. Materials and Methods: In this study 100 samples were collected from blood donors in Tehran. All samples were screened for anti-HTLV-I antibodies by ELISA. Then, genomic DNA was extracted from all positive samples (10 people), and for confirmation of infection, ordinary PCR was performed for both the HBZ and LTR regions. Moreover, the Tax region was amplified and purified PCR products were sequenced and analyzed, and finally, a phylogenetic tree was constructed using Mega X software. Results: Phylogenetic analysis confirmed that isolates from Iran, Japan, Brazil, and Africa are located within the extensive ‘‘transcontinental’’ subgroup A clade of HTLV-1 Cosmopolitan subtype a. The Japanese sequences are the closest to the Iranian sequences and have the most genetic similarity with them. Conclusion: Through phylogenetic and phylodynamic analyses HTLV-1 strain in Tehran were characterized in Iran. The appearance of HTLV-1 in Iran was probably happened by the ancient Silk Road which linked China to Antioch.https://ijm.tums.ac.ir/index.php/ijm/article/view/3051Human T-lymphotropic virus type-1;Adult T cell leukemia lymphoma;Phylogenetic;Phylodynamic;Iran |
| spellingShingle | Samira Pourrezaei Shahrzad Shadabi Maryam Gheidishahran Abbas Rahimiforoushani Masoume Akhbari Mahnaz Tavakoli Mahshid Safavi Mobina Madihi Mehdi Norouzi Molecular epidemiology and phylogenetic analysis of human T-lymphotropic virus type 1 in the tax gene and it association with adult t-cell leukemia/lymphoma disorders Iranian Journal of Microbiology Human T-lymphotropic virus type-1; Adult T cell leukemia lymphoma; Phylogenetic; Phylodynamic; Iran |
| title | Molecular epidemiology and phylogenetic analysis of human T-lymphotropic virus type 1 in the tax gene and it association with adult t-cell leukemia/lymphoma disorders |
| title_full | Molecular epidemiology and phylogenetic analysis of human T-lymphotropic virus type 1 in the tax gene and it association with adult t-cell leukemia/lymphoma disorders |
| title_fullStr | Molecular epidemiology and phylogenetic analysis of human T-lymphotropic virus type 1 in the tax gene and it association with adult t-cell leukemia/lymphoma disorders |
| title_full_unstemmed | Molecular epidemiology and phylogenetic analysis of human T-lymphotropic virus type 1 in the tax gene and it association with adult t-cell leukemia/lymphoma disorders |
| title_short | Molecular epidemiology and phylogenetic analysis of human T-lymphotropic virus type 1 in the tax gene and it association with adult t-cell leukemia/lymphoma disorders |
| title_sort | molecular epidemiology and phylogenetic analysis of human t lymphotropic virus type 1 in the tax gene and it association with adult t cell leukemia lymphoma disorders |
| topic | Human T-lymphotropic virus type-1; Adult T cell leukemia lymphoma; Phylogenetic; Phylodynamic; Iran |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/3051 |
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