<i>Aspergillus</i> Is Inhibited by <i>Pseudomonas aeruginosa</i> Volatiles
Background: <i>Pseudomonas aeruginosa</i> (Pa) and <i>Aspergillus fumigatus</i> (Af) compete with each other for nutrients and survival in natural environments, and have been extensively studied because of their intermicrobial interactions in the human microbiome. These are t...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/6/3/118 |
| _version_ | 1827712385866006528 |
|---|---|
| author | Hasan Nazik Gabriele Sass Eric Déziel David A. Stevens |
| author_facet | Hasan Nazik Gabriele Sass Eric Déziel David A. Stevens |
| author_sort | Hasan Nazik |
| collection | DOAJ |
| description | Background: <i>Pseudomonas aeruginosa</i> (Pa) and <i>Aspergillus fumigatus</i> (Af) compete with each other for nutrients and survival in natural environments, and have been extensively studied because of their intermicrobial interactions in the human microbiome. These are the principal microbes infecting immunocompromised patients and persons with cystic fibrosis, particularly the airways. These intermicrobial studies have largely been conducted in liquid medium or on agar, and thus focus on soluble or diffusible microbial products. Several key inhibitory molecules were defined in such studies. Methods: in the present report, we examine several methodologies which can be conveniently used to study the interaction of microbial volatiles, including capture methods and kinetics. Results: Pa volatiles inhibit Af, and the inhibitory mechanism appears to be the incorporation of the inhibitory molecules into the substrate nourishing the Af, rather than directly onto Af structures. We define by mass spectroscopy some specific volatile Pa products that can inhibit Af. Some of these molecules are selected for interest by the study of gene deletion mutants, producing a few Pa strains that were impaired in inhibition. We presumed the volatiles of these latter strains could be excluded from the search for inhibitors. Conclusion: the Pa inhibition of Af via a gaseous phase could be critical components in their competition, particularly in airways, where more direct contact may not be extensive. |
| first_indexed | 2024-03-10T18:13:14Z |
| format | Article |
| id | doaj.art-9fab827d15f5476683fa7245b15423a5 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T18:13:14Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-9fab827d15f5476683fa7245b15423a52023-11-20T07:56:10ZengMDPI AGJournal of Fungi2309-608X2020-07-016311810.3390/jof6030118<i>Aspergillus</i> Is Inhibited by <i>Pseudomonas aeruginosa</i> VolatilesHasan Nazik0Gabriele Sass1Eric Déziel2David A. Stevens3California Institute for Medical Research, San Jose, CA 95128, USACalifornia Institute for Medical Research, San Jose, CA 95128, USAInstitut National de la Recherche Scientifique, Institut Armand-Frappier, Laval, QC H7V 1B7, CanadaCalifornia Institute for Medical Research, San Jose, CA 95128, USABackground: <i>Pseudomonas aeruginosa</i> (Pa) and <i>Aspergillus fumigatus</i> (Af) compete with each other for nutrients and survival in natural environments, and have been extensively studied because of their intermicrobial interactions in the human microbiome. These are the principal microbes infecting immunocompromised patients and persons with cystic fibrosis, particularly the airways. These intermicrobial studies have largely been conducted in liquid medium or on agar, and thus focus on soluble or diffusible microbial products. Several key inhibitory molecules were defined in such studies. Methods: in the present report, we examine several methodologies which can be conveniently used to study the interaction of microbial volatiles, including capture methods and kinetics. Results: Pa volatiles inhibit Af, and the inhibitory mechanism appears to be the incorporation of the inhibitory molecules into the substrate nourishing the Af, rather than directly onto Af structures. We define by mass spectroscopy some specific volatile Pa products that can inhibit Af. Some of these molecules are selected for interest by the study of gene deletion mutants, producing a few Pa strains that were impaired in inhibition. We presumed the volatiles of these latter strains could be excluded from the search for inhibitors. Conclusion: the Pa inhibition of Af via a gaseous phase could be critical components in their competition, particularly in airways, where more direct contact may not be extensive.https://www.mdpi.com/2309-608X/6/3/118<i>Pseudomonas</i><i>Aspergillus</i>volatiles |
| spellingShingle | Hasan Nazik Gabriele Sass Eric Déziel David A. Stevens <i>Aspergillus</i> Is Inhibited by <i>Pseudomonas aeruginosa</i> Volatiles Journal of Fungi <i>Pseudomonas</i> <i>Aspergillus</i> volatiles |
| title | <i>Aspergillus</i> Is Inhibited by <i>Pseudomonas aeruginosa</i> Volatiles |
| title_full | <i>Aspergillus</i> Is Inhibited by <i>Pseudomonas aeruginosa</i> Volatiles |
| title_fullStr | <i>Aspergillus</i> Is Inhibited by <i>Pseudomonas aeruginosa</i> Volatiles |
| title_full_unstemmed | <i>Aspergillus</i> Is Inhibited by <i>Pseudomonas aeruginosa</i> Volatiles |
| title_short | <i>Aspergillus</i> Is Inhibited by <i>Pseudomonas aeruginosa</i> Volatiles |
| title_sort | i aspergillus i is inhibited by i pseudomonas aeruginosa i volatiles |
| topic | <i>Pseudomonas</i> <i>Aspergillus</i> volatiles |
| url | https://www.mdpi.com/2309-608X/6/3/118 |
| work_keys_str_mv | AT hasannazik iaspergillusiisinhibitedbyipseudomonasaeruginosaivolatiles AT gabrielesass iaspergillusiisinhibitedbyipseudomonasaeruginosaivolatiles AT ericdeziel iaspergillusiisinhibitedbyipseudomonasaeruginosaivolatiles AT davidastevens iaspergillusiisinhibitedbyipseudomonasaeruginosaivolatiles |