Intra-arterial delivery of doxorubicin-loaded hollow gold nanospheres—photothermal ablation and chemoembolisation therapy in a rabbit VX2 liver cancer model
Hepatocellular carcinoma (HCC) is associated with a high mortality rate. In this study, we aimed to investigate the therapeutic effect and safety of hepatic arterial administration of doxorubicin (Dox)-loaded hollow gold nanospheres (Dox@HAuNSs) combined with photothermal ablation (PTA) in a rabbit...
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| Format: | Article |
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Taylor & Francis Group
2022-12-01
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| Series: | Journal of Experimental Nanoscience |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/17458080.2022.2091132 |
| _version_ | 1811239108349001728 |
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| author | Xiaomei Tian Junsong Lian Xin Li Xianxian Chen Hongjun Yuan Min Zhou Fengyong Liu |
| author_facet | Xiaomei Tian Junsong Lian Xin Li Xianxian Chen Hongjun Yuan Min Zhou Fengyong Liu |
| author_sort | Xiaomei Tian |
| collection | DOAJ |
| description | Hepatocellular carcinoma (HCC) is associated with a high mortality rate. In this study, we aimed to investigate the therapeutic effect and safety of hepatic arterial administration of doxorubicin (Dox)-loaded hollow gold nanospheres (Dox@HAuNSs) combined with photothermal ablation (PTA) in a rabbit VX2 liver cancer model established by implanting VX2 tumour cells into rabbit livers. Rabbits were randomly divided into four treatment groups: physiological saline solution (control); lipiodol and doxorubicin (Dox + Lp); lipiodol and Dox@HAuNS (Dox@PEG-HAuNS + Lp); and lipiodol, Dox@PEG-HAuNS, and photothermal ablation (Dox@PEG-HAuNS + Lp + PTA). Dox release from Dox@PEG-HAuNS in the tumour was detected using fluorescence microscopy. Tumour size and liver-kidney function were assessed in each rabbit preoperatively and on postoperative days 3, 7, and 14. Necrosis, proliferation, and micro-vessel density of VX2 tumours were estimated in peripheral tumour tissues. Dox release from Dox@PEG-HAuNS was increased with a subsequent increase in tumour necrosis in liver tumours after PTA, whereas the tumour volume and proliferation decreased. However, the aspartate aminotransferase and alanine aminotransferase levels indicated transient liver damage. Thus, intra-arterial delivery of Dox@PEG-HAuNS combined with PTA can suppress VX2 liver cancer growth. Dox@PEG-HAuNS + Lp + PTA is also associated with transient liver damage in rabbits. The combination of Dox@PEG-HAuNS chemoembolisation and PTA could be a potential therapeutic approach for HCC and it has broad application prospects. |
| first_indexed | 2024-04-12T12:54:02Z |
| format | Article |
| id | doaj.art-9fb1223bec3f43288532292a6edaafb3 |
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| issn | 1745-8080 1745-8099 |
| language | English |
| last_indexed | 2024-04-12T12:54:02Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Experimental Nanoscience |
| spelling | doaj.art-9fb1223bec3f43288532292a6edaafb32022-12-22T03:32:23ZengTaylor & Francis GroupJournal of Experimental Nanoscience1745-80801745-80992022-12-0117143945010.1080/17458080.2022.2091132Intra-arterial delivery of doxorubicin-loaded hollow gold nanospheres—photothermal ablation and chemoembolisation therapy in a rabbit VX2 liver cancer modelXiaomei Tian0Junsong Lian1Xin Li2Xianxian Chen3Hongjun Yuan4Min Zhou5Fengyong Liu6Medical School of Chinese PLA, Beijing, ChinaNinth Department of Healthcare, the Second Medical Center, Beijing Key Laboratory of Aging and Geriatrics, National Clinical Research Center for Geriatrics Diseases, Chinese PLA General Hospital, Beijing, ChinaDepartment of Interventional Radiology, The Fifth Medical Center, Chinese PLA General Hospital, Beijing, ChinaDepartment of Interventional Radiology, Shenzhen People's Hospital, Shenzhen, ChinaDepartment of Interventional Radiology, The Fifth Medical Center, Chinese PLA General Hospital, Beijing, ChinaInstitute of Translational Medicine, Zhejiang University, Hangzhou, ChinaMedical School of Chinese PLA, Beijing, ChinaHepatocellular carcinoma (HCC) is associated with a high mortality rate. In this study, we aimed to investigate the therapeutic effect and safety of hepatic arterial administration of doxorubicin (Dox)-loaded hollow gold nanospheres (Dox@HAuNSs) combined with photothermal ablation (PTA) in a rabbit VX2 liver cancer model established by implanting VX2 tumour cells into rabbit livers. Rabbits were randomly divided into four treatment groups: physiological saline solution (control); lipiodol and doxorubicin (Dox + Lp); lipiodol and Dox@HAuNS (Dox@PEG-HAuNS + Lp); and lipiodol, Dox@PEG-HAuNS, and photothermal ablation (Dox@PEG-HAuNS + Lp + PTA). Dox release from Dox@PEG-HAuNS in the tumour was detected using fluorescence microscopy. Tumour size and liver-kidney function were assessed in each rabbit preoperatively and on postoperative days 3, 7, and 14. Necrosis, proliferation, and micro-vessel density of VX2 tumours were estimated in peripheral tumour tissues. Dox release from Dox@PEG-HAuNS was increased with a subsequent increase in tumour necrosis in liver tumours after PTA, whereas the tumour volume and proliferation decreased. However, the aspartate aminotransferase and alanine aminotransferase levels indicated transient liver damage. Thus, intra-arterial delivery of Dox@PEG-HAuNS combined with PTA can suppress VX2 liver cancer growth. Dox@PEG-HAuNS + Lp + PTA is also associated with transient liver damage in rabbits. The combination of Dox@PEG-HAuNS chemoembolisation and PTA could be a potential therapeutic approach for HCC and it has broad application prospects.https://www.tandfonline.com/doi/10.1080/17458080.2022.2091132Hollow gold nanospheretranscatheter arterial chemoembolisationphotothermal ablationangiogenesisVX2hepatocellular carcinoma |
| spellingShingle | Xiaomei Tian Junsong Lian Xin Li Xianxian Chen Hongjun Yuan Min Zhou Fengyong Liu Intra-arterial delivery of doxorubicin-loaded hollow gold nanospheres—photothermal ablation and chemoembolisation therapy in a rabbit VX2 liver cancer model Journal of Experimental Nanoscience Hollow gold nanosphere transcatheter arterial chemoembolisation photothermal ablation angiogenesis VX2 hepatocellular carcinoma |
| title | Intra-arterial delivery of doxorubicin-loaded hollow gold nanospheres—photothermal ablation and chemoembolisation therapy in a rabbit VX2 liver cancer model |
| title_full | Intra-arterial delivery of doxorubicin-loaded hollow gold nanospheres—photothermal ablation and chemoembolisation therapy in a rabbit VX2 liver cancer model |
| title_fullStr | Intra-arterial delivery of doxorubicin-loaded hollow gold nanospheres—photothermal ablation and chemoembolisation therapy in a rabbit VX2 liver cancer model |
| title_full_unstemmed | Intra-arterial delivery of doxorubicin-loaded hollow gold nanospheres—photothermal ablation and chemoembolisation therapy in a rabbit VX2 liver cancer model |
| title_short | Intra-arterial delivery of doxorubicin-loaded hollow gold nanospheres—photothermal ablation and chemoembolisation therapy in a rabbit VX2 liver cancer model |
| title_sort | intra arterial delivery of doxorubicin loaded hollow gold nanospheres photothermal ablation and chemoembolisation therapy in a rabbit vx2 liver cancer model |
| topic | Hollow gold nanosphere transcatheter arterial chemoembolisation photothermal ablation angiogenesis VX2 hepatocellular carcinoma |
| url | https://www.tandfonline.com/doi/10.1080/17458080.2022.2091132 |
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