Identifying gene expression profiles associated with neurogenesis and inflammation in the human subependymal zone from development through aging
Abstract The generation of new neurons within the mammalian forebrain continues throughout life within two main neurogenic niches, the subgranular zone (SGZ) of the hippocampal dentate gyrus, and the subependymal zone (SEZ) lining the lateral ventricles. Though the SEZ is the largest neurogenic nich...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-03976-4 |
| _version_ | 1798034743996973056 |
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| author | Mainá Bitar Christin Weissleder Hayley F. North Misaki S. Clearwater Oressia Zalucki Glenda M. Halliday Maree J. Webster Michael Piper Cynthia Shannon Weickert Guy Barry |
| author_facet | Mainá Bitar Christin Weissleder Hayley F. North Misaki S. Clearwater Oressia Zalucki Glenda M. Halliday Maree J. Webster Michael Piper Cynthia Shannon Weickert Guy Barry |
| author_sort | Mainá Bitar |
| collection | DOAJ |
| description | Abstract The generation of new neurons within the mammalian forebrain continues throughout life within two main neurogenic niches, the subgranular zone (SGZ) of the hippocampal dentate gyrus, and the subependymal zone (SEZ) lining the lateral ventricles. Though the SEZ is the largest neurogenic niche in the adult human forebrain, our understanding of the mechanisms regulating neurogenesis from development through aging within this region remains limited. This is especially pertinent given that neurogenesis declines dramatically over the postnatal lifespan. Here, we performed transcriptomic profiling on the SEZ from human post-mortem tissue from eight different life-stages ranging from neonates (average age ~ 2 months old) to aged adults (average age ~ 86 years old). We identified transcripts with concomitant profiles across these decades of life and focused on three of the most distinct profiles, namely (1) genes whose expression declined sharply after birth, (2) genes whose expression increased steadily with age, and (3) genes whose expression increased sharply in old age in the SEZ. Critically, these profiles identified neuroinflammation as becoming more prevalent with advancing age within the SEZ and occurring with time courses, one gradual (starting in mid-life) and one sharper (starting in old age). |
| first_indexed | 2024-04-11T20:48:26Z |
| format | Article |
| id | doaj.art-9fb28e833e2a4b5da74e8d0075ffd3b1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T20:48:26Z |
| publishDate | 2022-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9fb28e833e2a4b5da74e8d0075ffd3b12022-12-22T04:03:57ZengNature PortfolioScientific Reports2045-23222022-01-0112111210.1038/s41598-021-03976-4Identifying gene expression profiles associated with neurogenesis and inflammation in the human subependymal zone from development through agingMainá Bitar0Christin Weissleder1Hayley F. North2Misaki S. Clearwater3Oressia Zalucki4Glenda M. Halliday5Maree J. Webster6Michael Piper7Cynthia Shannon Weickert8Guy Barry9QIMR Berghofer Medical Research InstituteSchizophrenia Research Laboratory, Neuroscience Research AustraliaSchizophrenia Research Laboratory, Neuroscience Research AustraliaSchizophrenia Research Laboratory, Neuroscience Research AustraliaSchool of Biomedical Sciences, The University of QueenslandSchool of Medical Sciences, Faculty of Medicine, University of New South WalesLaboratory of Brain Research, Stanley Medical Research InstituteSchool of Biomedical Sciences, The University of QueenslandSchizophrenia Research Laboratory, Neuroscience Research AustraliaQIMR Berghofer Medical Research InstituteAbstract The generation of new neurons within the mammalian forebrain continues throughout life within two main neurogenic niches, the subgranular zone (SGZ) of the hippocampal dentate gyrus, and the subependymal zone (SEZ) lining the lateral ventricles. Though the SEZ is the largest neurogenic niche in the adult human forebrain, our understanding of the mechanisms regulating neurogenesis from development through aging within this region remains limited. This is especially pertinent given that neurogenesis declines dramatically over the postnatal lifespan. Here, we performed transcriptomic profiling on the SEZ from human post-mortem tissue from eight different life-stages ranging from neonates (average age ~ 2 months old) to aged adults (average age ~ 86 years old). We identified transcripts with concomitant profiles across these decades of life and focused on three of the most distinct profiles, namely (1) genes whose expression declined sharply after birth, (2) genes whose expression increased steadily with age, and (3) genes whose expression increased sharply in old age in the SEZ. Critically, these profiles identified neuroinflammation as becoming more prevalent with advancing age within the SEZ and occurring with time courses, one gradual (starting in mid-life) and one sharper (starting in old age).https://doi.org/10.1038/s41598-021-03976-4 |
| spellingShingle | Mainá Bitar Christin Weissleder Hayley F. North Misaki S. Clearwater Oressia Zalucki Glenda M. Halliday Maree J. Webster Michael Piper Cynthia Shannon Weickert Guy Barry Identifying gene expression profiles associated with neurogenesis and inflammation in the human subependymal zone from development through aging Scientific Reports |
| title | Identifying gene expression profiles associated with neurogenesis and inflammation in the human subependymal zone from development through aging |
| title_full | Identifying gene expression profiles associated with neurogenesis and inflammation in the human subependymal zone from development through aging |
| title_fullStr | Identifying gene expression profiles associated with neurogenesis and inflammation in the human subependymal zone from development through aging |
| title_full_unstemmed | Identifying gene expression profiles associated with neurogenesis and inflammation in the human subependymal zone from development through aging |
| title_short | Identifying gene expression profiles associated with neurogenesis and inflammation in the human subependymal zone from development through aging |
| title_sort | identifying gene expression profiles associated with neurogenesis and inflammation in the human subependymal zone from development through aging |
| url | https://doi.org/10.1038/s41598-021-03976-4 |
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