Analysis of Phaseolus vulgaris response to its association with Trichoderma harzianum (ALL-42) in the presence or absence of the phytopathogenic fungi Rhizoctonia solani and Fusarium solani.
The present study was carried out to evaluate the ability of Trichoderma harzianum (ALL 42-isolated from Brazilian Cerrado soil) to promote common bean growth and to modulate its metabolism and defense response in the presence or absence of the phytopathogenic fungi Rhizoctonia solani and Fusarium s...
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| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24878929/?tool=EBI |
| _version_ | 1819180930857697280 |
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| author | Jackeline L Pereira Rayner M L Queiroz Sébastien O Charneau Carlos R Felix Carlos A O Ricart Francilene Lopes da Silva Andrei Stecca Steindorff Cirano J Ulhoa Eliane F Noronha |
| author_facet | Jackeline L Pereira Rayner M L Queiroz Sébastien O Charneau Carlos R Felix Carlos A O Ricart Francilene Lopes da Silva Andrei Stecca Steindorff Cirano J Ulhoa Eliane F Noronha |
| author_sort | Jackeline L Pereira |
| collection | DOAJ |
| description | The present study was carried out to evaluate the ability of Trichoderma harzianum (ALL 42-isolated from Brazilian Cerrado soil) to promote common bean growth and to modulate its metabolism and defense response in the presence or absence of the phytopathogenic fungi Rhizoctonia solani and Fusarium solani using a proteomic approach. T. harzianum was able to promote common bean plants growth as shown by the increase in root/foliar areas and by size in comparison to plants grown in its absence. The interaction was shown to modulate the expression of defense-related genes (Glu1, pod3 and lox1) in roots of P. vulgaris. Proteomic maps constructed using roots and leaves of plants challenged or unchallenged by T. harzianum and phytopathogenic fungi showed differences. Reference gels presented differences in spot distribution (absence/presence) and relative volumes of common spots (up or down-regulation). Differential spots were identified by peptide fingerprinting MALDI-TOF mass spectrometry. A total of 48 identified spots (19 for leaves and 29 for roots) were grouped into protein functional classes. For leaves, 33%, 22% and 11% of the identified proteins were categorized as pertaining to the groups: metabolism, defense response and oxidative stress response, respectively. For roots, 17.2%, 24.1% and 10.3% of the identified proteins were categorized as pertaining to the groups: metabolism, defense response and oxidative stress response, respectively. |
| first_indexed | 2024-12-22T22:22:10Z |
| format | Article |
| id | doaj.art-9fb3588ef0044d45b768541ef95709fe |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T22:22:10Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9fb3588ef0044d45b768541ef95709fe2022-12-21T18:10:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0195e9823410.1371/journal.pone.0098234Analysis of Phaseolus vulgaris response to its association with Trichoderma harzianum (ALL-42) in the presence or absence of the phytopathogenic fungi Rhizoctonia solani and Fusarium solani.Jackeline L PereiraRayner M L QueirozSébastien O CharneauCarlos R FelixCarlos A O RicartFrancilene Lopes da SilvaAndrei Stecca SteindorffCirano J UlhoaEliane F NoronhaThe present study was carried out to evaluate the ability of Trichoderma harzianum (ALL 42-isolated from Brazilian Cerrado soil) to promote common bean growth and to modulate its metabolism and defense response in the presence or absence of the phytopathogenic fungi Rhizoctonia solani and Fusarium solani using a proteomic approach. T. harzianum was able to promote common bean plants growth as shown by the increase in root/foliar areas and by size in comparison to plants grown in its absence. The interaction was shown to modulate the expression of defense-related genes (Glu1, pod3 and lox1) in roots of P. vulgaris. Proteomic maps constructed using roots and leaves of plants challenged or unchallenged by T. harzianum and phytopathogenic fungi showed differences. Reference gels presented differences in spot distribution (absence/presence) and relative volumes of common spots (up or down-regulation). Differential spots were identified by peptide fingerprinting MALDI-TOF mass spectrometry. A total of 48 identified spots (19 for leaves and 29 for roots) were grouped into protein functional classes. For leaves, 33%, 22% and 11% of the identified proteins were categorized as pertaining to the groups: metabolism, defense response and oxidative stress response, respectively. For roots, 17.2%, 24.1% and 10.3% of the identified proteins were categorized as pertaining to the groups: metabolism, defense response and oxidative stress response, respectively.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24878929/?tool=EBI |
| spellingShingle | Jackeline L Pereira Rayner M L Queiroz Sébastien O Charneau Carlos R Felix Carlos A O Ricart Francilene Lopes da Silva Andrei Stecca Steindorff Cirano J Ulhoa Eliane F Noronha Analysis of Phaseolus vulgaris response to its association with Trichoderma harzianum (ALL-42) in the presence or absence of the phytopathogenic fungi Rhizoctonia solani and Fusarium solani. PLoS ONE |
| title | Analysis of Phaseolus vulgaris response to its association with Trichoderma harzianum (ALL-42) in the presence or absence of the phytopathogenic fungi Rhizoctonia solani and Fusarium solani. |
| title_full | Analysis of Phaseolus vulgaris response to its association with Trichoderma harzianum (ALL-42) in the presence or absence of the phytopathogenic fungi Rhizoctonia solani and Fusarium solani. |
| title_fullStr | Analysis of Phaseolus vulgaris response to its association with Trichoderma harzianum (ALL-42) in the presence or absence of the phytopathogenic fungi Rhizoctonia solani and Fusarium solani. |
| title_full_unstemmed | Analysis of Phaseolus vulgaris response to its association with Trichoderma harzianum (ALL-42) in the presence or absence of the phytopathogenic fungi Rhizoctonia solani and Fusarium solani. |
| title_short | Analysis of Phaseolus vulgaris response to its association with Trichoderma harzianum (ALL-42) in the presence or absence of the phytopathogenic fungi Rhizoctonia solani and Fusarium solani. |
| title_sort | analysis of phaseolus vulgaris response to its association with trichoderma harzianum all 42 in the presence or absence of the phytopathogenic fungi rhizoctonia solani and fusarium solani |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24878929/?tool=EBI |
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