In vivo characterization of 3D-printed polycaprolactone-hydroxyapatite scaffolds with Voronoi design to advance the concept of scaffold-guided bone regeneration
Three-dimensional (3D)-printed medical-grade polycaprolactone (mPCL) composite scaffolds have been the first to enable the concept of scaffold-guided bone regeneration (SGBR) from bench to bedside. However, advances in 3D printing technologies now promise next-generation scaffolds such as those with...
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1272348/full |
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| author | Markus Laubach Markus Laubach Markus Laubach Buddhi Herath Buddhi Herath Buddhi Herath Nathalie Bock Nathalie Bock Nathalie Bock Sinduja Suresh Sinduja Suresh Sinduja Suresh Siamak Saifzadeh Siamak Saifzadeh Siamak Saifzadeh Bronwin L. Dargaville Bronwin L. Dargaville Jacqui McGovern Jacqui McGovern Jacqui McGovern Marie-Luise Wille Marie-Luise Wille Marie-Luise Wille Dietmar W. Hutmacher Dietmar W. Hutmacher Dietmar W. Hutmacher Dietmar W. Hutmacher Flavia Medeiros Savi Flavia Medeiros Savi Flavia Medeiros Savi |
| author_facet | Markus Laubach Markus Laubach Markus Laubach Buddhi Herath Buddhi Herath Buddhi Herath Nathalie Bock Nathalie Bock Nathalie Bock Sinduja Suresh Sinduja Suresh Sinduja Suresh Siamak Saifzadeh Siamak Saifzadeh Siamak Saifzadeh Bronwin L. Dargaville Bronwin L. Dargaville Jacqui McGovern Jacqui McGovern Jacqui McGovern Marie-Luise Wille Marie-Luise Wille Marie-Luise Wille Dietmar W. Hutmacher Dietmar W. Hutmacher Dietmar W. Hutmacher Dietmar W. Hutmacher Flavia Medeiros Savi Flavia Medeiros Savi Flavia Medeiros Savi |
| author_sort | Markus Laubach |
| collection | DOAJ |
| description | Three-dimensional (3D)-printed medical-grade polycaprolactone (mPCL) composite scaffolds have been the first to enable the concept of scaffold-guided bone regeneration (SGBR) from bench to bedside. However, advances in 3D printing technologies now promise next-generation scaffolds such as those with Voronoi tessellation. We hypothesized that the combination of a Voronoi design, applied for the first time to 3D-printed mPCL and ceramic fillers (here hydroxyapatite, HA), would allow slow degradation and high osteogenicity needed to regenerate bone tissue and enhance regenerative properties when mixed with xenograft material. We tested this hypothesis in vitro and in vivo using 3D-printed composite mPCL-HA scaffolds (wt 96%:4%) with the Voronoi design using an ISO 13485 certified additive manufacturing platform. The resulting scaffold porosity was 73% and minimal in vitro degradation (mass loss <1%) was observed over the period of 6 months. After loading the scaffolds with different types of fresh sheep xenograft and ectopic implantation in rats for 8 weeks, highly vascularized tissue without extensive fibrous encapsulation was found in all mPCL-HA Voronoi scaffolds and endochondral bone formation was observed, with no adverse host-tissue reactions. This study supports the use of mPCL-HA Voronoi scaffolds for further testing in future large preclinical animal studies prior to clinical trials to ultimately successfully advance the SGBR concept. |
| first_indexed | 2024-03-11T19:58:43Z |
| format | Article |
| id | doaj.art-9fb3b19f31c0442d8cffb7d30c342589 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-11T19:58:43Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-9fb3b19f31c0442d8cffb7d30c3425892023-10-04T13:58:02ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-10-011110.3389/fbioe.2023.12723481272348In vivo characterization of 3D-printed polycaprolactone-hydroxyapatite scaffolds with Voronoi design to advance the concept of scaffold-guided bone regenerationMarkus Laubach0Markus Laubach1Markus Laubach2Buddhi Herath3Buddhi Herath4Buddhi Herath5Nathalie Bock6Nathalie Bock7Nathalie Bock8Sinduja Suresh9Sinduja Suresh10Sinduja Suresh11Siamak Saifzadeh12Siamak Saifzadeh13Siamak Saifzadeh14Bronwin L. Dargaville15Bronwin L. Dargaville16Jacqui McGovern17Jacqui McGovern18Jacqui McGovern19Marie-Luise Wille20Marie-Luise Wille21Marie-Luise Wille22Dietmar W. Hutmacher23Dietmar W. Hutmacher24Dietmar W. Hutmacher25Dietmar W. Hutmacher26Flavia Medeiros Savi27Flavia Medeiros Savi28Flavia Medeiros Savi29Australian Research Council (ARC) Training Centre for Multiscale 3D Imaging, Modelling, and Manufacturing (M3D Innovation), Queensland University of Technology, Brisbane, QLD, AustraliaCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD, AustraliaDepartment of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), LMU University Hospital, LMU Munich, Munich, GermanyAustralian Research Council (ARC) Training Centre for Multiscale 3D Imaging, Modelling, and Manufacturing (M3D Innovation), Queensland University of Technology, Brisbane, QLD, AustraliaCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD, AustraliaJamieson Trauma Institute, Metro North Hospital and Health Service, Royal Brisbane and Women’s Hospital, Herston, QLD, AustraliaAustralian Research Council (ARC) Training Centre for Multiscale 3D Imaging, Modelling, and Manufacturing (M3D Innovation), Queensland University of Technology, Brisbane, QLD, AustraliaCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD, AustraliaMax Planck Queensland Centre (MPQC) for the Materials Science of Extracellular Matrices, Queensland University of Technology, Brisbane, QLD, AustraliaAustralian Research Council (ARC) Training Centre for Multiscale 3D Imaging, Modelling, and Manufacturing (M3D Innovation), Queensland University of Technology, Brisbane, QLD, AustraliaCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD, AustraliaBiomechanics and Spine Research Group at the Centre of Children’s Health Research, Queensland University of Technology, Brisbane, QLD, AustraliaAustralian Research Council (ARC) Training Centre for Multiscale 3D Imaging, Modelling, and Manufacturing (M3D Innovation), Queensland University of Technology, Brisbane, QLD, AustraliaCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD, AustraliaMedical Engineering Research Facility, Queensland University of Technology, Chermside, QLD, AustraliaCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD, AustraliaMax Planck Queensland Centre (MPQC) for the Materials Science of Extracellular Matrices, Queensland University of Technology, Brisbane, QLD, AustraliaCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD, AustraliaMax Planck Queensland Centre (MPQC) for the Materials Science of Extracellular Matrices, Queensland University of Technology, Brisbane, QLD, AustraliaARC Training Centre for Cell and Tissue Engineering Technologies, Queensland University of Technology, Brisbane, QLD, AustraliaAustralian Research Council (ARC) Training Centre for Multiscale 3D Imaging, Modelling, and Manufacturing (M3D Innovation), Queensland University of Technology, Brisbane, QLD, AustraliaCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD, AustraliaMax Planck Queensland Centre (MPQC) for the Materials Science of Extracellular Matrices, Queensland University of Technology, Brisbane, QLD, AustraliaAustralian Research Council (ARC) Training Centre for Multiscale 3D Imaging, Modelling, and Manufacturing (M3D Innovation), Queensland University of Technology, Brisbane, QLD, AustraliaCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD, AustraliaMax Planck Queensland Centre (MPQC) for the Materials Science of Extracellular Matrices, Queensland University of Technology, Brisbane, QLD, AustraliaARC Training Centre for Cell and Tissue Engineering Technologies, Queensland University of Technology, Brisbane, QLD, AustraliaAustralian Research Council (ARC) Training Centre for Multiscale 3D Imaging, Modelling, and Manufacturing (M3D Innovation), Queensland University of Technology, Brisbane, QLD, AustraliaCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD, AustraliaMax Planck Queensland Centre (MPQC) for the Materials Science of Extracellular Matrices, Queensland University of Technology, Brisbane, QLD, AustraliaThree-dimensional (3D)-printed medical-grade polycaprolactone (mPCL) composite scaffolds have been the first to enable the concept of scaffold-guided bone regeneration (SGBR) from bench to bedside. However, advances in 3D printing technologies now promise next-generation scaffolds such as those with Voronoi tessellation. We hypothesized that the combination of a Voronoi design, applied for the first time to 3D-printed mPCL and ceramic fillers (here hydroxyapatite, HA), would allow slow degradation and high osteogenicity needed to regenerate bone tissue and enhance regenerative properties when mixed with xenograft material. We tested this hypothesis in vitro and in vivo using 3D-printed composite mPCL-HA scaffolds (wt 96%:4%) with the Voronoi design using an ISO 13485 certified additive manufacturing platform. The resulting scaffold porosity was 73% and minimal in vitro degradation (mass loss <1%) was observed over the period of 6 months. After loading the scaffolds with different types of fresh sheep xenograft and ectopic implantation in rats for 8 weeks, highly vascularized tissue without extensive fibrous encapsulation was found in all mPCL-HA Voronoi scaffolds and endochondral bone formation was observed, with no adverse host-tissue reactions. This study supports the use of mPCL-HA Voronoi scaffolds for further testing in future large preclinical animal studies prior to clinical trials to ultimately successfully advance the SGBR concept.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1272348/fullscaffoldpolycaprolactonehydroxyapatiteVoronoi3D printingscaffold-guided bone regeneration |
| spellingShingle | Markus Laubach Markus Laubach Markus Laubach Buddhi Herath Buddhi Herath Buddhi Herath Nathalie Bock Nathalie Bock Nathalie Bock Sinduja Suresh Sinduja Suresh Sinduja Suresh Siamak Saifzadeh Siamak Saifzadeh Siamak Saifzadeh Bronwin L. Dargaville Bronwin L. Dargaville Jacqui McGovern Jacqui McGovern Jacqui McGovern Marie-Luise Wille Marie-Luise Wille Marie-Luise Wille Dietmar W. Hutmacher Dietmar W. Hutmacher Dietmar W. Hutmacher Dietmar W. Hutmacher Flavia Medeiros Savi Flavia Medeiros Savi Flavia Medeiros Savi In vivo characterization of 3D-printed polycaprolactone-hydroxyapatite scaffolds with Voronoi design to advance the concept of scaffold-guided bone regeneration Frontiers in Bioengineering and Biotechnology scaffold polycaprolactone hydroxyapatite Voronoi 3D printing scaffold-guided bone regeneration |
| title | In vivo characterization of 3D-printed polycaprolactone-hydroxyapatite scaffolds with Voronoi design to advance the concept of scaffold-guided bone regeneration |
| title_full | In vivo characterization of 3D-printed polycaprolactone-hydroxyapatite scaffolds with Voronoi design to advance the concept of scaffold-guided bone regeneration |
| title_fullStr | In vivo characterization of 3D-printed polycaprolactone-hydroxyapatite scaffolds with Voronoi design to advance the concept of scaffold-guided bone regeneration |
| title_full_unstemmed | In vivo characterization of 3D-printed polycaprolactone-hydroxyapatite scaffolds with Voronoi design to advance the concept of scaffold-guided bone regeneration |
| title_short | In vivo characterization of 3D-printed polycaprolactone-hydroxyapatite scaffolds with Voronoi design to advance the concept of scaffold-guided bone regeneration |
| title_sort | in vivo characterization of 3d printed polycaprolactone hydroxyapatite scaffolds with voronoi design to advance the concept of scaffold guided bone regeneration |
| topic | scaffold polycaprolactone hydroxyapatite Voronoi 3D printing scaffold-guided bone regeneration |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1272348/full |
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