Comparison of N-Acetyl Cysteine and Resveratrol Treatments in Acetaminophen Toxicity Model in Rabbits
Purpose: Acetaminophen is safe analgesic drug but it may cause hepatic failure in high doses. N-acetyl cysteine (NAC) is used in the treatment of acetaminophen intoxication. Resveratrol may be used in experimental acetaminophen intoxication models. Purpose, compare the efficacy of NAC and resveratro...
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| Format: | Article |
| Language: | English |
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Kirsehir Ahi Evran University
2022-12-01
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| Series: | Ahi Evran Medical Journal |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/2200798 |
| _version_ | 1797916926075207680 |
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| author | Hasan Gazi UYAR Aysegül BAYIR Hasan KARA Pınar KARABAĞLI Abdullah SİVRİKAYA Ali UNLU |
| author_facet | Hasan Gazi UYAR Aysegül BAYIR Hasan KARA Pınar KARABAĞLI Abdullah SİVRİKAYA Ali UNLU |
| author_sort | Hasan Gazi UYAR |
| collection | DOAJ |
| description | Purpose: Acetaminophen is safe analgesic drug but it may cause hepatic failure in high doses. N-acetyl cysteine (NAC) is used in the treatment of acetaminophen intoxication. Resveratrol may be used in experimental acetaminophen intoxication models. Purpose, compare the efficacy of NAC and resveratrol treatments in acetaminophen intoxication.
Materials and Methods: We used 34 rabbits. Six rabbits were included in control group, and 7 rabbits were enrolled in other groups Asetaminophen (APAP) (N=7) group. The animals were administered 2 g/kg of acetaminophen by an orogastric tube. Asetaminophen+N-acetylcysteine (APAP+NAC) (N=7) group, 150 mg/kg NAC was administered intramuscular (i.m) to animals at 1st hour following 2 g/kg of acetaminophen administration by orogastric tube. Asetaminophen+resveratrol (APAP+RES) (N=7) group, animals received 100 mg/kg resveratrol by orogastric tube at 1st hour following oral administration of 2 g/kg of acetaminophen by orogastric tube. e. Asetaminophen+N-acetyl cysteine+resveratrol (APAP+NAC + RES) (N=7) group, animals received 100 mg/kg resveratrol by orogastric tube and 150 mg/kg NAC i.m at 1st hour following oral administration of 2 g/kg of acetaminophen by orogastric tube. ALT, AST, GSH, and acetaminophen levels were measured. Animals were sacrificed at the 24th hour. Liver samples were obtained for histopathological examination.
Results: According to our results, the GSH level at 12th hour was significantly different between APAP+NAC group and APAP+RES group (p=0.007). In histopathological examination, HSCORE level we used for apoptosis was statistically significantly lower in control group than in APAP group (p=0.007). Its supports that acetaminophen toxicity triggers apoptosis in liver cells.
Conclusion: Resveratrol may be an alternative treatment option to NAC in the treatment of acetaminophen toxicity. |
| first_indexed | 2024-04-10T13:06:28Z |
| format | Article |
| id | doaj.art-9fb81bb1c417470ca5830a280609b364 |
| institution | Directory Open Access Journal |
| issn | 2619-9203 |
| language | English |
| last_indexed | 2024-04-10T13:06:28Z |
| publishDate | 2022-12-01 |
| publisher | Kirsehir Ahi Evran University |
| record_format | Article |
| series | Ahi Evran Medical Journal |
| spelling | doaj.art-9fb81bb1c417470ca5830a280609b3642023-02-15T16:12:58ZengKirsehir Ahi Evran UniversityAhi Evran Medical Journal2619-92032022-12-016328228910.46332/aemj.1060025Comparison of N-Acetyl Cysteine and Resveratrol Treatments in Acetaminophen Toxicity Model in RabbitsHasan Gazi UYAR0https://orcid.org/0000-0003-4291-5017Aysegül BAYIR1https://orcid.org/0000-0002-5680-031XHasan KARA2https://orcid.org/0000-0002-3839-7651Pınar KARABAĞLI3https://orcid.org/0000-0002-5558-0175Abdullah SİVRİKAYA4https://orcid.org/0000-0003-2956-5681Ali UNLU5https://orcid.org/0000-0002-9991-3939KONYA MERAM DEVLET HASTANESİSELÇUK ÜNİVERSİTESİ, TIP FAKÜLTESİSELÇUK ÜNİVERSİTESİ, TIP FAKÜLTESİSELÇUK ÜNİVERSİTESİ, TIP FAKÜLTESİSELÇUK ÜNİVERSİTESİ, TIP FAKÜLTESİSELÇUK ÜNİVERSİTESİ, TIP FAKÜLTESİPurpose: Acetaminophen is safe analgesic drug but it may cause hepatic failure in high doses. N-acetyl cysteine (NAC) is used in the treatment of acetaminophen intoxication. Resveratrol may be used in experimental acetaminophen intoxication models. Purpose, compare the efficacy of NAC and resveratrol treatments in acetaminophen intoxication. Materials and Methods: We used 34 rabbits. Six rabbits were included in control group, and 7 rabbits were enrolled in other groups Asetaminophen (APAP) (N=7) group. The animals were administered 2 g/kg of acetaminophen by an orogastric tube. Asetaminophen+N-acetylcysteine (APAP+NAC) (N=7) group, 150 mg/kg NAC was administered intramuscular (i.m) to animals at 1st hour following 2 g/kg of acetaminophen administration by orogastric tube. Asetaminophen+resveratrol (APAP+RES) (N=7) group, animals received 100 mg/kg resveratrol by orogastric tube at 1st hour following oral administration of 2 g/kg of acetaminophen by orogastric tube. e. Asetaminophen+N-acetyl cysteine+resveratrol (APAP+NAC + RES) (N=7) group, animals received 100 mg/kg resveratrol by orogastric tube and 150 mg/kg NAC i.m at 1st hour following oral administration of 2 g/kg of acetaminophen by orogastric tube. ALT, AST, GSH, and acetaminophen levels were measured. Animals were sacrificed at the 24th hour. Liver samples were obtained for histopathological examination. Results: According to our results, the GSH level at 12th hour was significantly different between APAP+NAC group and APAP+RES group (p=0.007). In histopathological examination, HSCORE level we used for apoptosis was statistically significantly lower in control group than in APAP group (p=0.007). Its supports that acetaminophen toxicity triggers apoptosis in liver cells. Conclusion: Resveratrol may be an alternative treatment option to NAC in the treatment of acetaminophen toxicity.https://dergipark.org.tr/tr/download/article-file/2200798resveratrolacetaminophenparacetamolnactoxicity |
| spellingShingle | Hasan Gazi UYAR Aysegül BAYIR Hasan KARA Pınar KARABAĞLI Abdullah SİVRİKAYA Ali UNLU Comparison of N-Acetyl Cysteine and Resveratrol Treatments in Acetaminophen Toxicity Model in Rabbits Ahi Evran Medical Journal resveratrol acetaminophen paracetamol nac toxicity |
| title | Comparison of N-Acetyl Cysteine and Resveratrol Treatments in Acetaminophen Toxicity Model in Rabbits |
| title_full | Comparison of N-Acetyl Cysteine and Resveratrol Treatments in Acetaminophen Toxicity Model in Rabbits |
| title_fullStr | Comparison of N-Acetyl Cysteine and Resveratrol Treatments in Acetaminophen Toxicity Model in Rabbits |
| title_full_unstemmed | Comparison of N-Acetyl Cysteine and Resveratrol Treatments in Acetaminophen Toxicity Model in Rabbits |
| title_short | Comparison of N-Acetyl Cysteine and Resveratrol Treatments in Acetaminophen Toxicity Model in Rabbits |
| title_sort | comparison of n acetyl cysteine and resveratrol treatments in acetaminophen toxicity model in rabbits |
| topic | resveratrol acetaminophen paracetamol nac toxicity |
| url | https://dergipark.org.tr/tr/download/article-file/2200798 |
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