Biological properties and polyphenols content of Algerian Cistus salviifolius L. aerial parts
This study evaluated the in vitro antioxidant properties, antibacterial and antifungal activities and in vivo anti-inflammatory properties, and identifying the phenolic compounds in Cistus salviifolius. The methanolic leaf extract showed the highest antioxidant activity with 6.1±1.60 µg/ml IC50 val...
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TMKarpinski Publisher, Tomasz M. Karpiński
2022-05-01
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| Series: | European Journal of Biological Research |
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| Online Access: | https://journals.tmkarpinski.com/index.php/ejbr/article/view/567 |
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| author | Sihem Boubekeur Mohammed Messaoudi Chinaza Godswill Awuchi Olutosin Ademola Otekunrin Barbara Sawicka Samira Idjeri-Mecherara Sihem Bouchareb Aicha Hassani Majid Sharifi-Rad Samir Begaa Abdelkrim Rebiai |
| author_facet | Sihem Boubekeur Mohammed Messaoudi Chinaza Godswill Awuchi Olutosin Ademola Otekunrin Barbara Sawicka Samira Idjeri-Mecherara Sihem Bouchareb Aicha Hassani Majid Sharifi-Rad Samir Begaa Abdelkrim Rebiai |
| author_sort | Sihem Boubekeur |
| collection | DOAJ |
| description |
This study evaluated the in vitro antioxidant properties, antibacterial and antifungal activities and in vivo anti-inflammatory properties, and identifying the phenolic compounds in Cistus salviifolius. The methanolic leaf extract showed the highest antioxidant activity with 6.1±1.60 µg/ml IC50 value using DPPH· and 55.5±0.20 µg/ml using Reducing Power Activity. The study revealed that the butanolic leaf extract and the aqueous leaf infusion exhibited the strongest growth-inhibiting effect against all Gram positive and Gram negative strains tested, respectively, whereas the methanolic leaf extract showed the strongest antifungal activity against the yeast tested. The MIC value for the butanolic leaf extract was 4 mg/ml against Staphylococcus aureus, Bacillus subtilis and Escherichia coli. The pharmacotoxicological tests proved the safety of the aqueous leaf infusion, which exhibit a moderate anti-inflammatory effect, with a significant inhibition of the oedema development equal to 44.7% compared to 59.3% for the reference product diclofenac sodium. Methanolic extracts of the leaf and flower buds showed varied contents of polyphenols, flavonoids, and hydrolysable tannins; which were 228.4±11.4 mg GAE/g, 34.2±0.6 mg QE/g, and 36.9±2.6 mg TAE/g of the dry weight for leaves; and 241.1±5.4 mg GAE/g, 47.6±4.5 mg QE/g, and 22.0±1.3 mg TAE/g of the dry weight for flower buds, respectively. Analysis of the ethereal and butanolic leaf extracts using Reversed Phase High Performance Liquid Chromatographic Method coupled with a Photodiode-Array Detector identified thirteen phenolic compounds, including ascorbic acid, vanillic acid, gallic acid, quercetin, and orientin.
DOI: http://dx.doi.org/10.5281/zenodo.6561505
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| first_indexed | 2024-04-12T17:18:00Z |
| format | Article |
| id | doaj.art-9fbdd83e471f4f929f91f36de78e7c30 |
| institution | Directory Open Access Journal |
| issn | 2449-8955 |
| language | English |
| last_indexed | 2024-04-12T17:18:00Z |
| publishDate | 2022-05-01 |
| publisher | TMKarpinski Publisher, Tomasz M. Karpiński |
| record_format | Article |
| series | European Journal of Biological Research |
| spelling | doaj.art-9fbdd83e471f4f929f91f36de78e7c302022-12-22T03:23:35ZengTMKarpinski Publisher, Tomasz M. KarpińskiEuropean Journal of Biological Research2449-89552022-05-01122Biological properties and polyphenols content of Algerian Cistus salviifolius L. aerial partsSihem Boubekeur0Mohammed Messaoudi1Chinaza Godswill Awuchi2Olutosin Ademola Otekunrin3Barbara Sawicka4Samira Idjeri-Mecherara5Sihem Bouchareb6Aicha Hassani7Majid Sharifi-Rad8Samir Begaa9Abdelkrim Rebiai10Laboratory of Research on Bio-active Products and Valorisation of Biomass, Higher Normal School El Bachir El Ibrahimi (E.N.S), Kouba- Algiers, BP 92, Algeria; Reserch and Development Centre RDC SAIDAL, 35 Benyoucef Khattab’s Avenue, BP 16000 Mohammadia, El-Harrach, Algiers, AlgeriaNuclear Research Centre of Birine, P.o. Box 180, Ain Oussera, 17200 Djelfa, Algeria; Chemistry Department, University of Hamma Lakhdar El-Oued, B.P.789, 39000, AlgeriaSchool of Natural and Applied Sciences, Kampala International University, Kampala, UgandaAgricultural Economics and Farm Management, Federal University of Agriculture, Abeokuta (FUNAAB), NigeriaDepartment of Plant Production Technology and Commodities Science, University of Life Science in Lublin, Akademicka 15 Str., 20-950 Lublin, PolandDepartment of Chemistry, Faculty of Chemistry, Laboratory of Functional Organic Analysis, Houari Boumediene University of Sciences and Technology (USTHB), El Alia, BP 32, Bab Ezzouar, 16111 Algiers, AlgeriaReserch and Development Centre RDC SAIDAL, 35 Benyoucef Khattab’s Avenue, BP 16000 Mohammadia, El-Harrach, Algiers, AlgeriaLaboratory of Research on Bio-active Products and Valorisation of Biomass, Higher Normal School El Bachir El Ibrahimi (E.N.S), Kouba- Algiers, BP 92, AlgeriaDepartment of Range and Watershed Management, Faculty of Water and Soil, University of Zabol, Zabol 98613-35856, IranNuclear Research Centre of Birine, P.o. Box 180, Ain Oussera, 17200 Djelfa, AlgeriaChemistry Department, University of Hamma Lakhdar El-Oued, B.P.789, 39000, Algeria This study evaluated the in vitro antioxidant properties, antibacterial and antifungal activities and in vivo anti-inflammatory properties, and identifying the phenolic compounds in Cistus salviifolius. The methanolic leaf extract showed the highest antioxidant activity with 6.1±1.60 µg/ml IC50 value using DPPH· and 55.5±0.20 µg/ml using Reducing Power Activity. The study revealed that the butanolic leaf extract and the aqueous leaf infusion exhibited the strongest growth-inhibiting effect against all Gram positive and Gram negative strains tested, respectively, whereas the methanolic leaf extract showed the strongest antifungal activity against the yeast tested. The MIC value for the butanolic leaf extract was 4 mg/ml against Staphylococcus aureus, Bacillus subtilis and Escherichia coli. The pharmacotoxicological tests proved the safety of the aqueous leaf infusion, which exhibit a moderate anti-inflammatory effect, with a significant inhibition of the oedema development equal to 44.7% compared to 59.3% for the reference product diclofenac sodium. Methanolic extracts of the leaf and flower buds showed varied contents of polyphenols, flavonoids, and hydrolysable tannins; which were 228.4±11.4 mg GAE/g, 34.2±0.6 mg QE/g, and 36.9±2.6 mg TAE/g of the dry weight for leaves; and 241.1±5.4 mg GAE/g, 47.6±4.5 mg QE/g, and 22.0±1.3 mg TAE/g of the dry weight for flower buds, respectively. Analysis of the ethereal and butanolic leaf extracts using Reversed Phase High Performance Liquid Chromatographic Method coupled with a Photodiode-Array Detector identified thirteen phenolic compounds, including ascorbic acid, vanillic acid, gallic acid, quercetin, and orientin. DOI: http://dx.doi.org/10.5281/zenodo.6561505 https://journals.tmkarpinski.com/index.php/ejbr/article/view/567Antioxidant activityCistus salviifoliusPhytochemistryTotal phenol contentBiological activity |
| spellingShingle | Sihem Boubekeur Mohammed Messaoudi Chinaza Godswill Awuchi Olutosin Ademola Otekunrin Barbara Sawicka Samira Idjeri-Mecherara Sihem Bouchareb Aicha Hassani Majid Sharifi-Rad Samir Begaa Abdelkrim Rebiai Biological properties and polyphenols content of Algerian Cistus salviifolius L. aerial parts European Journal of Biological Research Antioxidant activity Cistus salviifolius Phytochemistry Total phenol content Biological activity |
| title | Biological properties and polyphenols content of Algerian Cistus salviifolius L. aerial parts |
| title_full | Biological properties and polyphenols content of Algerian Cistus salviifolius L. aerial parts |
| title_fullStr | Biological properties and polyphenols content of Algerian Cistus salviifolius L. aerial parts |
| title_full_unstemmed | Biological properties and polyphenols content of Algerian Cistus salviifolius L. aerial parts |
| title_short | Biological properties and polyphenols content of Algerian Cistus salviifolius L. aerial parts |
| title_sort | biological properties and polyphenols content of algerian cistus salviifolius l aerial parts |
| topic | Antioxidant activity Cistus salviifolius Phytochemistry Total phenol content Biological activity |
| url | https://journals.tmkarpinski.com/index.php/ejbr/article/view/567 |
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