Open-source computational framework for studying Drosophila behavioral phase

Summary: This protocol describes a standardized method for analyzing Drosophila behavioral rhythmicity under light dark cycles, temperature ramps, and free running conditions. The protocol constitutes a step-by-step guide from generation of appropriate Drosophila genetic crosses to behavioral experi...

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Bibliographic Details
Main Authors: Boyan Kostadinov, Hannah Lee Pettibone, Evardra Valerie Bell, Xiaona Zhou, Ausra Pranevicius, Orie Thomas Shafer, Maria Paz Fernandez
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302720
Description
Summary:Summary: This protocol describes a standardized method for analyzing Drosophila behavioral rhythmicity under light dark cycles, temperature ramps, and free running conditions. The protocol constitutes a step-by-step guide from generation of appropriate Drosophila genetic crosses to behavioral experiments. We also provide an open-source computational framework using R for the analysis of the phase of behavior using circular statistics. An extended method for complete use is also provided.For complete details on the use and execution of this protocol, please refer to Fernandez et al. (2020).
ISSN:2666-1667