Open-source computational framework for studying Drosophila behavioral phase
Summary: This protocol describes a standardized method for analyzing Drosophila behavioral rhythmicity under light dark cycles, temperature ramps, and free running conditions. The protocol constitutes a step-by-step guide from generation of appropriate Drosophila genetic crosses to behavioral experi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720302720 |
| _version_ | 1819163708612411392 |
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| author | Boyan Kostadinov Hannah Lee Pettibone Evardra Valerie Bell Xiaona Zhou Ausra Pranevicius Orie Thomas Shafer Maria Paz Fernandez |
| author_facet | Boyan Kostadinov Hannah Lee Pettibone Evardra Valerie Bell Xiaona Zhou Ausra Pranevicius Orie Thomas Shafer Maria Paz Fernandez |
| author_sort | Boyan Kostadinov |
| collection | DOAJ |
| description | Summary: This protocol describes a standardized method for analyzing Drosophila behavioral rhythmicity under light dark cycles, temperature ramps, and free running conditions. The protocol constitutes a step-by-step guide from generation of appropriate Drosophila genetic crosses to behavioral experiments. We also provide an open-source computational framework using R for the analysis of the phase of behavior using circular statistics. An extended method for complete use is also provided.For complete details on the use and execution of this protocol, please refer to Fernandez et al. (2020). |
| first_indexed | 2024-12-22T17:48:26Z |
| format | Article |
| id | doaj.art-9fbe23397bd54e58a50c5d4b3fdf1945 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-22T17:48:26Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-9fbe23397bd54e58a50c5d4b3fdf19452022-12-21T18:18:15ZengElsevierSTAR Protocols2666-16672021-03-0121100285Open-source computational framework for studying Drosophila behavioral phaseBoyan Kostadinov0Hannah Lee Pettibone1Evardra Valerie Bell2Xiaona Zhou3Ausra Pranevicius4Orie Thomas Shafer5Maria Paz Fernandez6Mathematics Department, NYC College of Technology, City University of New York, Brooklyn, NY 11201, USA; Corresponding authorAdvanced Science Research Center, The Graduate Center, City University of New York, New York, NY 10031, USADepartment of Neuroscience and Behavior, Barnard College of Columbia University, New York, NY 10027, USAMathematics Department, NYC College of Technology, City University of New York, Brooklyn, NY 11201, USADepartment of Neuroscience and Behavior, Barnard College of Columbia University, New York, NY 10027, USAAdvanced Science Research Center, The Graduate Center, City University of New York, New York, NY 10031, USADepartment of Neuroscience and Behavior, Barnard College of Columbia University, New York, NY 10027, USA; Corresponding authorSummary: This protocol describes a standardized method for analyzing Drosophila behavioral rhythmicity under light dark cycles, temperature ramps, and free running conditions. The protocol constitutes a step-by-step guide from generation of appropriate Drosophila genetic crosses to behavioral experiments. We also provide an open-source computational framework using R for the analysis of the phase of behavior using circular statistics. An extended method for complete use is also provided.For complete details on the use and execution of this protocol, please refer to Fernandez et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720302720BioinformaticsModel organismsNeuroscience |
| spellingShingle | Boyan Kostadinov Hannah Lee Pettibone Evardra Valerie Bell Xiaona Zhou Ausra Pranevicius Orie Thomas Shafer Maria Paz Fernandez Open-source computational framework for studying Drosophila behavioral phase STAR Protocols Bioinformatics Model organisms Neuroscience |
| title | Open-source computational framework for studying Drosophila behavioral phase |
| title_full | Open-source computational framework for studying Drosophila behavioral phase |
| title_fullStr | Open-source computational framework for studying Drosophila behavioral phase |
| title_full_unstemmed | Open-source computational framework for studying Drosophila behavioral phase |
| title_short | Open-source computational framework for studying Drosophila behavioral phase |
| title_sort | open source computational framework for studying drosophila behavioral phase |
| topic | Bioinformatics Model organisms Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720302720 |
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