Protective effect of dexmedetomidine on endothelial barrier function in rats with sepsis
Objective To investigate the protective effects of dexmedetomidine on endothelial barrier function in rats with sepsis. Methods Sprague-Dawley rats were randomly divided into sham operation group, sepsis group, sepsis+conventional treatment group, and sepsis+dexmedetomidine group. In all but the sha...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Third Military Medical University
2020-02-01
|
| Series: | Di-san junyi daxue xuebao |
| Subjects: | |
| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/201909226.htm |
| _version_ | 1818909138766266368 |
|---|---|
| author | SHE Han FANG He LI Tao LIU Liangming HU Yi |
| author_facet | SHE Han FANG He LI Tao LIU Liangming HU Yi |
| author_sort | SHE Han |
| collection | DOAJ |
| description | Objective To investigate the protective effects of dexmedetomidine on endothelial barrier function in rats with sepsis. Methods Sprague-Dawley rats were randomly divided into sham operation group, sepsis group, sepsis+conventional treatment group, and sepsis+dexmedetomidine group. In all but the sham operatioin group, the rats were subjected to cecal ligation and puncture (CLP) to induce sepsis and were given corresponding treatments. The vascular permeability of the mesenteric microvessels and the lungs was examined and the survival time and rate of the rats were recorded. In the cell experiment, the effect of dexmedetomidine on the expression of zonula occludes-1 (ZO-1) and transmembrane electrical resistance (TER) was observed in pulmonary vein endothelial cells stimulated with lipopolysaccharide (LPS). Results Compared with the sham-operated rats, the rats receiving CLP had significantly increased vascular permeability of the lungs and mesenteric microvessels with shortened survival time and a lowered survival rate (P < 0.01). Conventional treatment only slightly reduced the vascular permeability in rats with sepsis (P>0.05). Dexmedetomidine treatment significantly decreased vascular permeability and increased the survival time and rate of the septic rats (P < 0.01). In the pulmonary vein endothelial cells, LPS stimulation significantly decreased the expression of ZO-1, caused obvious damage of the intercellular junctions and significantly reduced TER; these changes were significantly rescued by dexmedetomidine treatment. Conclusion Dexmedetomidine can significantly improve the endothelial barrier function in rats with sepsis. |
| first_indexed | 2024-12-19T22:22:09Z |
| format | Article |
| id | doaj.art-9fc04d5576074b6890d58babc23cf9b2 |
| institution | Directory Open Access Journal |
| issn | 1000-5404 |
| language | zho |
| last_indexed | 2024-12-19T22:22:09Z |
| publishDate | 2020-02-01 |
| publisher | Editorial Office of Journal of Third Military Medical University |
| record_format | Article |
| series | Di-san junyi daxue xuebao |
| spelling | doaj.art-9fc04d5576074b6890d58babc23cf9b22022-12-21T20:03:37ZzhoEditorial Office of Journal of Third Military Medical UniversityDi-san junyi daxue xuebao1000-54042020-02-0142433434110.16016/j.1000-5404.201909226Protective effect of dexmedetomidine on endothelial barrier function in rats with sepsisSHE Han0FANG He1LI Tao2LIU Liangming3HU Yi4Department of Anesthesiology, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, 400042, ChinaDepartment of Anesthesiology, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, 400042, ChinaState Key Laboratory of Trauma, Burns and Combined Injury, Department of War Wound Shock and Blood Transfusion, Institute of Surgery Research, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, 400042, China State Key Laboratory of Trauma, Burns and Combined Injury, Department of War Wound Shock and Blood Transfusion, Institute of Surgery Research, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, 400042, China Department of Anesthesiology, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, 400042, China;Objective To investigate the protective effects of dexmedetomidine on endothelial barrier function in rats with sepsis. Methods Sprague-Dawley rats were randomly divided into sham operation group, sepsis group, sepsis+conventional treatment group, and sepsis+dexmedetomidine group. In all but the sham operatioin group, the rats were subjected to cecal ligation and puncture (CLP) to induce sepsis and were given corresponding treatments. The vascular permeability of the mesenteric microvessels and the lungs was examined and the survival time and rate of the rats were recorded. In the cell experiment, the effect of dexmedetomidine on the expression of zonula occludes-1 (ZO-1) and transmembrane electrical resistance (TER) was observed in pulmonary vein endothelial cells stimulated with lipopolysaccharide (LPS). Results Compared with the sham-operated rats, the rats receiving CLP had significantly increased vascular permeability of the lungs and mesenteric microvessels with shortened survival time and a lowered survival rate (P < 0.01). Conventional treatment only slightly reduced the vascular permeability in rats with sepsis (P>0.05). Dexmedetomidine treatment significantly decreased vascular permeability and increased the survival time and rate of the septic rats (P < 0.01). In the pulmonary vein endothelial cells, LPS stimulation significantly decreased the expression of ZO-1, caused obvious damage of the intercellular junctions and significantly reduced TER; these changes were significantly rescued by dexmedetomidine treatment. Conclusion Dexmedetomidine can significantly improve the endothelial barrier function in rats with sepsis.http://aammt.tmmu.edu.cn/Upload/rhtml/201909226.htmdexmedetomidinesepsisendothelial barrier functionzonula occludes-1pulmonary vein endothelialcellsrats |
| spellingShingle | SHE Han FANG He LI Tao LIU Liangming HU Yi Protective effect of dexmedetomidine on endothelial barrier function in rats with sepsis Di-san junyi daxue xuebao dexmedetomidine sepsis endothelial barrier function zonula occludes-1 pulmonary vein endothelial cells rats |
| title | Protective effect of dexmedetomidine on endothelial barrier function in rats with sepsis |
| title_full | Protective effect of dexmedetomidine on endothelial barrier function in rats with sepsis |
| title_fullStr | Protective effect of dexmedetomidine on endothelial barrier function in rats with sepsis |
| title_full_unstemmed | Protective effect of dexmedetomidine on endothelial barrier function in rats with sepsis |
| title_short | Protective effect of dexmedetomidine on endothelial barrier function in rats with sepsis |
| title_sort | protective effect of dexmedetomidine on endothelial barrier function in rats with sepsis |
| topic | dexmedetomidine sepsis endothelial barrier function zonula occludes-1 pulmonary vein endothelial cells rats |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/201909226.htm |
| work_keys_str_mv | AT shehan protectiveeffectofdexmedetomidineonendothelialbarrierfunctioninratswithsepsis AT fanghe protectiveeffectofdexmedetomidineonendothelialbarrierfunctioninratswithsepsis AT litao protectiveeffectofdexmedetomidineonendothelialbarrierfunctioninratswithsepsis AT liuliangming protectiveeffectofdexmedetomidineonendothelialbarrierfunctioninratswithsepsis AT huyi protectiveeffectofdexmedetomidineonendothelialbarrierfunctioninratswithsepsis |