RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines
Abstract Background Gibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species...
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| Format: | Article |
| Language: | English |
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BMC
2019-02-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-019-1675-4 |
| _version_ | 1818143604169768960 |
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| author | Jie Arro Yingzhen Yang Guo-Qing Song Gan-Yuan Zhong |
| author_facet | Jie Arro Yingzhen Yang Guo-Qing Song Gan-Yuan Zhong |
| author_sort | Jie Arro |
| collection | DOAJ |
| description | Abstract Background Gibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species, we created ten GA-insensitive transgenic grapevines carrying a DELLA mutant allele (Vvgai1) in the background of Vitis vinifera ‘Thompson Seedless’ and conducted comprehensive analysis of their RNA expression profiles in the shoot, leaf and root tissues. Results The transgenic lines showed varying degrees of dwarf stature and other typical DELLA mutant phenotypes tightly correlated with the levels of Vvgai1 expression. A large number of differentially expressed genes (DEGs) were identified in the shoot, leaf and root tissues of the transgenic lines and these DEGs were involved in diverse biological processes; many of the DEGs showed strong tissue specificity and about 30% them carried a DELLA motif. We further discovered unexpected expression patterns of several key flowering induction genes VvCO, VvCOL1 and VvTFL1. Conclusions Our results not only confirmed many previous DELLA study findings in annual species, but also revealed new DELLA targets and responses in grapevine, including the roles of homeodomain transcription factors as potential co-regulators with DELLA in controlling the development of grapevine which uniquely possess both vegetative and reproductive meristems at the same time. The contrasting responses of some key flowering induction pathway genes provides new insights into the divergence of GA-DELLA regulations between annual and perennial species in GA-DELLA signaling. |
| first_indexed | 2024-12-11T11:34:18Z |
| format | Article |
| id | doaj.art-9fc7b01e82dc4cd0967c4aa61461c814 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-11T11:34:18Z |
| publishDate | 2019-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-9fc7b01e82dc4cd0967c4aa61461c8142022-12-22T01:08:48ZengBMCBMC Plant Biology1471-22292019-02-0119111610.1186/s12870-019-1675-4RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevinesJie Arro0Yingzhen Yang1Guo-Qing Song2Gan-Yuan Zhong3USDA-ARS Grape Genetics Research UnitUSDA-ARS Grape Genetics Research UnitDepartment of Horticulture, Michigan State UniversityUSDA-ARS Grape Genetics Research UnitAbstract Background Gibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species, we created ten GA-insensitive transgenic grapevines carrying a DELLA mutant allele (Vvgai1) in the background of Vitis vinifera ‘Thompson Seedless’ and conducted comprehensive analysis of their RNA expression profiles in the shoot, leaf and root tissues. Results The transgenic lines showed varying degrees of dwarf stature and other typical DELLA mutant phenotypes tightly correlated with the levels of Vvgai1 expression. A large number of differentially expressed genes (DEGs) were identified in the shoot, leaf and root tissues of the transgenic lines and these DEGs were involved in diverse biological processes; many of the DEGs showed strong tissue specificity and about 30% them carried a DELLA motif. We further discovered unexpected expression patterns of several key flowering induction genes VvCO, VvCOL1 and VvTFL1. Conclusions Our results not only confirmed many previous DELLA study findings in annual species, but also revealed new DELLA targets and responses in grapevine, including the roles of homeodomain transcription factors as potential co-regulators with DELLA in controlling the development of grapevine which uniquely possess both vegetative and reproductive meristems at the same time. The contrasting responses of some key flowering induction pathway genes provides new insights into the divergence of GA-DELLA regulations between annual and perennial species in GA-DELLA signaling.http://link.springer.com/article/10.1186/s12870-019-1675-4CONSTANSDELLAFloweringGrapevineHOMEODOMAINPerennial |
| spellingShingle | Jie Arro Yingzhen Yang Guo-Qing Song Gan-Yuan Zhong RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines BMC Plant Biology CONSTANS DELLA Flowering Grapevine HOMEODOMAIN Perennial |
| title | RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines |
| title_full | RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines |
| title_fullStr | RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines |
| title_full_unstemmed | RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines |
| title_short | RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines |
| title_sort | rna seq reveals new della targets and regulation in transgenic ga insensitive grapevines |
| topic | CONSTANS DELLA Flowering Grapevine HOMEODOMAIN Perennial |
| url | http://link.springer.com/article/10.1186/s12870-019-1675-4 |
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