| Summary: | In this study, we identified Plasmopara-viticola-lesion-associated mononegaambi virus 3 (recently classified as <i>Penicillimonavirus gammaplasmoparae</i>), a fungi-associated mymonavirus, in grapevine plants showing an unusual upward curling symptomatology on the leaves and premature decline. <i>Mymonaviridae</i> is a family comprising nine genera of negative-sense single-stranded RNA viruses infecting filamentous fungi, although few of them have been associated with oomycetes, plants, and insects. Although the first mymonavirus genome description was reported a decade ago, the genome organization of several genera in the family, including the genus <i>Penicillimonavirus,</i> has remained unclear to date. We have determined the complete genome of <i>P. gammaplasmoparae,</i> which represents the first complete genomic sequence for this genus. Moreover, we provide strong evidence that <i>P. gammaplasmoparae</i> genome is bipartite and comprises two RNA molecules of around 6150 and 4560 nt. Our results indicate that the grapevine powdery mildew pathogen, <i>Erysiphe necator,</i> was also present in the analyzed plants and suggest <i>P. gammaplasmoparae</i> could be infecting this fungus. However, whether the fungus and/or the mycovirus are associated with the symptomatology that initially prompted these efforts remains to be determined.
|
|---|