Copy number variations of five Y chromosome genes in donkeys
In mammals, the Y chromosome plays a pivotal role in male sex determination and is essential for normal sperm production. A number of studies were conducted on Y chromosome genes of various species and identified single-copy and multi-copy genes. However, limited studies about donkey Y chromosom...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2017-10-01
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| Series: | Archives Animal Breeding |
| Online Access: | https://www.arch-anim-breed.net/60/391/2017/aab-60-391-2017.pdf |
| Summary: | In mammals, the Y chromosome plays a pivotal role in male sex
determination and is essential for normal sperm production. A number of
studies were conducted on Y chromosome genes of various species and
identified single-copy and multi-copy genes. However, limited studies
about donkey Y chromosome genes have been done. In this study, 263 male
samples from 13 Chinese donkey breeds were collected to analyze the copy
number variations (CNVs) of five Y chromosome genes using the quantitative PCR
(qPCR) method. These five genes (cullin 4 B Y (<i>CUL4BY</i>),
equus testis-specific transcript y1 (<i>ETSTY1</i>),
equus testis-specific transcript y4 (<i>ETSTY4</i>), equus testis-specific transcript Y 5
(<i>ETSTY5</i>), and sex-determining region Y (<i>SRY</i>) were identified as
multi-copy, whose median copy numbers (MCNs) were 5, 45, 2, and 2, and
13 with CNV ranges of 1–57, 1–227, 1–37, 1–86 and 1–152, respectively. The
CNVs of these five genes were shared in different breeds. Compared to
previous studies, the copy numbers of five genes showed some distinct
consequences in this study. In particular, the well-known single-copy <i>SRY</i> gene
showed CNVs in donkeys. Our results provided genetic variations of donkey Y
chromosome genes. |
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| ISSN: | 0003-9438 2363-9822 |