Chondroitin Sulfate Prevents STZ Induced Diabetic Osteoporosis through Decreasing Blood Glucose, AntiOxidative Stress, Anti-Inflammation and OPG/RANKL Expression Regulation
Chondroitin sulfate (CS) has antioxidative, anti-inflammatory, anti-osteoarthritic and hypoglycemic effects. However, whether it has antidiabetic osteoporosis effects has not been reported. Therefore, in this study, we established a STZ-induced diabetic rat model; CS (500 mg kg<sup>−1</sup&...
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MDPI AG
2020-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/15/5303 |
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| author | Hong Xing Zheng De Jing Chen Yue Xin Zu En Zhu Wang Shan Shan Qi |
| author_facet | Hong Xing Zheng De Jing Chen Yue Xin Zu En Zhu Wang Shan Shan Qi |
| author_sort | Hong Xing Zheng |
| collection | DOAJ |
| description | Chondroitin sulfate (CS) has antioxidative, anti-inflammatory, anti-osteoarthritic and hypoglycemic effects. However, whether it has antidiabetic osteoporosis effects has not been reported. Therefore, in this study, we established a STZ-induced diabetic rat model; CS (500 mg kg<sup>−1</sup> d<sup>−1</sup>) was orally administrated for eight weeks to study its preventive effects on diabetic osteoporosis. The results showed that eight weeks of CS treatment improved the symptoms of diabetes; the CS-treated group has increased body weight, decreased water or food intake, decreased blood glucose, increased bone-mineral density, repaired bone morphology and decreased femoral osteoclasts and tibia adipocytes numbers. After CS treatment, bone histomorphometric parameters returned to normal, the levels of serum inflammatory cytokines (IL-1β, IL-6 and TNF-α) decreased significantly, serum SOD, GPX and CAT activities increased and MDA level increased. In the CS-treated group, the levels of serum ALP, CTX-1, TRACP 5b, osteocalcin and RANKL decreased and the serum RUNX 2 and OPG levels increased. Bone immunohistochemistry results showed that CS can effectively increase the expression of OPG and RUNX2 and reduce the expression of RANKL in diabetic rats. All of these indicate that CS could prevent STZ induced diabetic osteoporosis—mainly through decreasing blood glucose, antioxidative stress, anti-inflammation and regulation of OPG/RANKL expression. CS can therefore effectively prevent bone loss caused by diabetes. |
| first_indexed | 2024-03-10T18:12:16Z |
| format | Article |
| id | doaj.art-9fcb68a625d14135b461f51987df4783 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T18:12:16Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9fcb68a625d14135b461f51987df47832023-11-20T08:01:47ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-07-012115530310.3390/ijms21155303Chondroitin Sulfate Prevents STZ Induced Diabetic Osteoporosis through Decreasing Blood Glucose, AntiOxidative Stress, Anti-Inflammation and OPG/RANKL Expression RegulationHong Xing Zheng0De Jing Chen1Yue Xin Zu2En Zhu Wang3Shan Shan Qi4College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaCollege of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaCollege of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaCollege of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaCollege of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaChondroitin sulfate (CS) has antioxidative, anti-inflammatory, anti-osteoarthritic and hypoglycemic effects. However, whether it has antidiabetic osteoporosis effects has not been reported. Therefore, in this study, we established a STZ-induced diabetic rat model; CS (500 mg kg<sup>−1</sup> d<sup>−1</sup>) was orally administrated for eight weeks to study its preventive effects on diabetic osteoporosis. The results showed that eight weeks of CS treatment improved the symptoms of diabetes; the CS-treated group has increased body weight, decreased water or food intake, decreased blood glucose, increased bone-mineral density, repaired bone morphology and decreased femoral osteoclasts and tibia adipocytes numbers. After CS treatment, bone histomorphometric parameters returned to normal, the levels of serum inflammatory cytokines (IL-1β, IL-6 and TNF-α) decreased significantly, serum SOD, GPX and CAT activities increased and MDA level increased. In the CS-treated group, the levels of serum ALP, CTX-1, TRACP 5b, osteocalcin and RANKL decreased and the serum RUNX 2 and OPG levels increased. Bone immunohistochemistry results showed that CS can effectively increase the expression of OPG and RUNX2 and reduce the expression of RANKL in diabetic rats. All of these indicate that CS could prevent STZ induced diabetic osteoporosis—mainly through decreasing blood glucose, antioxidative stress, anti-inflammation and regulation of OPG/RANKL expression. CS can therefore effectively prevent bone loss caused by diabetes.https://www.mdpi.com/1422-0067/21/15/5303diabetic osteoporosischondroitin sulfateoxidative stressbone-mineral density |
| spellingShingle | Hong Xing Zheng De Jing Chen Yue Xin Zu En Zhu Wang Shan Shan Qi Chondroitin Sulfate Prevents STZ Induced Diabetic Osteoporosis through Decreasing Blood Glucose, AntiOxidative Stress, Anti-Inflammation and OPG/RANKL Expression Regulation International Journal of Molecular Sciences diabetic osteoporosis chondroitin sulfate oxidative stress bone-mineral density |
| title | Chondroitin Sulfate Prevents STZ Induced Diabetic Osteoporosis through Decreasing Blood Glucose, AntiOxidative Stress, Anti-Inflammation and OPG/RANKL Expression Regulation |
| title_full | Chondroitin Sulfate Prevents STZ Induced Diabetic Osteoporosis through Decreasing Blood Glucose, AntiOxidative Stress, Anti-Inflammation and OPG/RANKL Expression Regulation |
| title_fullStr | Chondroitin Sulfate Prevents STZ Induced Diabetic Osteoporosis through Decreasing Blood Glucose, AntiOxidative Stress, Anti-Inflammation and OPG/RANKL Expression Regulation |
| title_full_unstemmed | Chondroitin Sulfate Prevents STZ Induced Diabetic Osteoporosis through Decreasing Blood Glucose, AntiOxidative Stress, Anti-Inflammation and OPG/RANKL Expression Regulation |
| title_short | Chondroitin Sulfate Prevents STZ Induced Diabetic Osteoporosis through Decreasing Blood Glucose, AntiOxidative Stress, Anti-Inflammation and OPG/RANKL Expression Regulation |
| title_sort | chondroitin sulfate prevents stz induced diabetic osteoporosis through decreasing blood glucose antioxidative stress anti inflammation and opg rankl expression regulation |
| topic | diabetic osteoporosis chondroitin sulfate oxidative stress bone-mineral density |
| url | https://www.mdpi.com/1422-0067/21/15/5303 |
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