Generation of human induced pluripotent stem cell (iPSC) lines (UUMCBi001-A, UUMCBi002-A) from two healthy donors
Availability of numerous high-quality iPSC lines is needed to overcome donor-associated variability caused by genetic background effects. We generated two human iPSC lines from dermal fibroblasts of two healthy females using Sendai virus reprogramming. Quality assessment of the iPSC lines confirmed...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506120304153 |
| _version_ | 1818557036232704000 |
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| author | Svitlana Vasylovska Jens Schuster Anja Brboric Per-Ola Carlsson Niklas Dahl Joey Lau |
| author_facet | Svitlana Vasylovska Jens Schuster Anja Brboric Per-Ola Carlsson Niklas Dahl Joey Lau |
| author_sort | Svitlana Vasylovska |
| collection | DOAJ |
| description | Availability of numerous high-quality iPSC lines is needed to overcome donor-associated variability caused by genetic background effects. We generated two human iPSC lines from dermal fibroblasts of two healthy females using Sendai virus reprogramming. Quality assessment of the iPSC lines confirmed the expression of pluripotency markers, trilineage differentiation capacity and absence of exogenous expression of reprogramming factors. Both iPSC lines were genetically stable with a genotype that matched the fibroblast lines of donors. These iPSC lines add to available reference lines as a resource for disease modeling of polygenic and multifactorial diseases, for evaluation of differentiation protocols and toxicology screening. |
| first_indexed | 2024-12-13T23:54:52Z |
| format | Article |
| id | doaj.art-9fcc366cee2f4929a348efaf825fd1ad |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-13T23:54:52Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-9fcc366cee2f4929a348efaf825fd1ad2022-12-21T23:26:37ZengElsevierStem Cell Research1873-50612021-01-0150102114Generation of human induced pluripotent stem cell (iPSC) lines (UUMCBi001-A, UUMCBi002-A) from two healthy donorsSvitlana Vasylovska0Jens Schuster1Anja Brboric2Per-Ola Carlsson3Niklas Dahl4Joey Lau5Uppsala University, Department of Medical Cell Biology, Uppsala, SwedenUppsala University, Department of Immunology, Genetics and Pathology and Science for Life Laboratory, Uppsala, SwedenUppsala University, Department of Medical Cell Biology, Uppsala, SwedenUppsala University, Department of Medical Cell Biology, Uppsala, SwedenUppsala University, Department of Immunology, Genetics and Pathology and Science for Life Laboratory, Uppsala, SwedenUppsala University, Department of Medical Cell Biology, Uppsala, Sweden; Corresponding author.Availability of numerous high-quality iPSC lines is needed to overcome donor-associated variability caused by genetic background effects. We generated two human iPSC lines from dermal fibroblasts of two healthy females using Sendai virus reprogramming. Quality assessment of the iPSC lines confirmed the expression of pluripotency markers, trilineage differentiation capacity and absence of exogenous expression of reprogramming factors. Both iPSC lines were genetically stable with a genotype that matched the fibroblast lines of donors. These iPSC lines add to available reference lines as a resource for disease modeling of polygenic and multifactorial diseases, for evaluation of differentiation protocols and toxicology screening.http://www.sciencedirect.com/science/article/pii/S1873506120304153 |
| spellingShingle | Svitlana Vasylovska Jens Schuster Anja Brboric Per-Ola Carlsson Niklas Dahl Joey Lau Generation of human induced pluripotent stem cell (iPSC) lines (UUMCBi001-A, UUMCBi002-A) from two healthy donors Stem Cell Research |
| title | Generation of human induced pluripotent stem cell (iPSC) lines (UUMCBi001-A, UUMCBi002-A) from two healthy donors |
| title_full | Generation of human induced pluripotent stem cell (iPSC) lines (UUMCBi001-A, UUMCBi002-A) from two healthy donors |
| title_fullStr | Generation of human induced pluripotent stem cell (iPSC) lines (UUMCBi001-A, UUMCBi002-A) from two healthy donors |
| title_full_unstemmed | Generation of human induced pluripotent stem cell (iPSC) lines (UUMCBi001-A, UUMCBi002-A) from two healthy donors |
| title_short | Generation of human induced pluripotent stem cell (iPSC) lines (UUMCBi001-A, UUMCBi002-A) from two healthy donors |
| title_sort | generation of human induced pluripotent stem cell ipsc lines uumcbi001 a uumcbi002 a from two healthy donors |
| url | http://www.sciencedirect.com/science/article/pii/S1873506120304153 |
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