Evaluation of Antimicrobial, Antiadhesive and Co-Aggregation Activity of a Multi-Strain Probiotic Composition against Different Urogenital Pathogens
The urogenital microbiota is dominated by <i>Lactobacillus</i> that, together with <i>Bifidobacterium</i>, creates a physiological barrier counteracting pathogen infections. The aim of this study was to evaluate the efficacy of a multi-strain probiotic formulation (<i>L...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/2/1323 |
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| author | Patrizia Malfa Laura Brambilla Silvana Giardina Martina Masciarelli Diletta Francesca Squarzanti Federica Carlomagno Marisa Meloni |
| author_facet | Patrizia Malfa Laura Brambilla Silvana Giardina Martina Masciarelli Diletta Francesca Squarzanti Federica Carlomagno Marisa Meloni |
| author_sort | Patrizia Malfa |
| collection | DOAJ |
| description | The urogenital microbiota is dominated by <i>Lactobacillus</i> that, together with <i>Bifidobacterium</i>, creates a physiological barrier counteracting pathogen infections. The aim of this study was to evaluate the efficacy of a multi-strain probiotic formulation (<i>Lactiplantibacillus plantarum</i> PBS067, <i>Lacticaseibacillus rhamnosus</i> LRH020, and <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> BL050) to inhibit adhesion and growth of urogenital pathogens. The antimicrobial and antiadhesive properties of the probiotic strains and their mixture were evaluated on human vaginal epithelium infected with <i>Candida glabrata</i>, <i>Neisseria gonorrheae</i>, <i>Trichomonas vaginalis</i>, and <i>Escherichia coli</i>-infected human bladder epithelium. The epithelial tissue permeability and integrity were assessed by transepithelial/transendothelial electrical resistance (TEER). Co-aggregation between probiotics and vaginal pathogens was also investigated to elucidate a possible mechanism of action. The multi-strain formulation showed a full inhibition of <i>T. vaginalis</i>, and a reduction in <i>C. glabrata</i> and <i>N. gonorrheae</i> growth. A relevant antimicrobial activity was observed for each single strain against <i>E. coli</i>. TEER results demonstrated that none of the strains have negatively impaired the integrity of the 3D tissues. All the probiotics and their mixture were able to form aggregates with the tested pathogens. The study demonstrated that the three strains and their mixture are effective to prevent urogenital infections. |
| first_indexed | 2024-03-09T12:23:00Z |
| format | Article |
| id | doaj.art-9fccdacf45604f6e8891047d44371e7a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:23:00Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9fccdacf45604f6e8891047d44371e7a2023-11-30T22:38:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01242132310.3390/ijms24021323Evaluation of Antimicrobial, Antiadhesive and Co-Aggregation Activity of a Multi-Strain Probiotic Composition against Different Urogenital PathogensPatrizia Malfa0Laura Brambilla1Silvana Giardina2Martina Masciarelli3Diletta Francesca Squarzanti4Federica Carlomagno5Marisa Meloni6SynBalance srl, 21040 Origgio, VA, ItalyVitroScreen srl, 20149 Milan, MI, ItalyComplife Italia srl, 20024 Garbagnate Milanese, MI, ItalyComplife Italia srl, 20024 Garbagnate Milanese, MI, ItalySynBalance srl, 21040 Origgio, VA, ItalyRoelmi HPC srl, 21040 Origgio, VA, ItalyVitroScreen srl, 20149 Milan, MI, ItalyThe urogenital microbiota is dominated by <i>Lactobacillus</i> that, together with <i>Bifidobacterium</i>, creates a physiological barrier counteracting pathogen infections. The aim of this study was to evaluate the efficacy of a multi-strain probiotic formulation (<i>Lactiplantibacillus plantarum</i> PBS067, <i>Lacticaseibacillus rhamnosus</i> LRH020, and <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> BL050) to inhibit adhesion and growth of urogenital pathogens. The antimicrobial and antiadhesive properties of the probiotic strains and their mixture were evaluated on human vaginal epithelium infected with <i>Candida glabrata</i>, <i>Neisseria gonorrheae</i>, <i>Trichomonas vaginalis</i>, and <i>Escherichia coli</i>-infected human bladder epithelium. The epithelial tissue permeability and integrity were assessed by transepithelial/transendothelial electrical resistance (TEER). Co-aggregation between probiotics and vaginal pathogens was also investigated to elucidate a possible mechanism of action. The multi-strain formulation showed a full inhibition of <i>T. vaginalis</i>, and a reduction in <i>C. glabrata</i> and <i>N. gonorrheae</i> growth. A relevant antimicrobial activity was observed for each single strain against <i>E. coli</i>. TEER results demonstrated that none of the strains have negatively impaired the integrity of the 3D tissues. All the probiotics and their mixture were able to form aggregates with the tested pathogens. The study demonstrated that the three strains and their mixture are effective to prevent urogenital infections.https://www.mdpi.com/1422-0067/24/2/1323urogenital microbiotaprobioticspathogensinfectionantimicrobial activityco-aggregation |
| spellingShingle | Patrizia Malfa Laura Brambilla Silvana Giardina Martina Masciarelli Diletta Francesca Squarzanti Federica Carlomagno Marisa Meloni Evaluation of Antimicrobial, Antiadhesive and Co-Aggregation Activity of a Multi-Strain Probiotic Composition against Different Urogenital Pathogens International Journal of Molecular Sciences urogenital microbiota probiotics pathogens infection antimicrobial activity co-aggregation |
| title | Evaluation of Antimicrobial, Antiadhesive and Co-Aggregation Activity of a Multi-Strain Probiotic Composition against Different Urogenital Pathogens |
| title_full | Evaluation of Antimicrobial, Antiadhesive and Co-Aggregation Activity of a Multi-Strain Probiotic Composition against Different Urogenital Pathogens |
| title_fullStr | Evaluation of Antimicrobial, Antiadhesive and Co-Aggregation Activity of a Multi-Strain Probiotic Composition against Different Urogenital Pathogens |
| title_full_unstemmed | Evaluation of Antimicrobial, Antiadhesive and Co-Aggregation Activity of a Multi-Strain Probiotic Composition against Different Urogenital Pathogens |
| title_short | Evaluation of Antimicrobial, Antiadhesive and Co-Aggregation Activity of a Multi-Strain Probiotic Composition against Different Urogenital Pathogens |
| title_sort | evaluation of antimicrobial antiadhesive and co aggregation activity of a multi strain probiotic composition against different urogenital pathogens |
| topic | urogenital microbiota probiotics pathogens infection antimicrobial activity co-aggregation |
| url | https://www.mdpi.com/1422-0067/24/2/1323 |
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