Exploratory Investigation of Intestinal Function and Bacterial Translocation After Focal Cerebral Ischemia in the Mouse
Background and Purpose: The gut communicates with the brain bidirectionally via neural, humoral and immune pathways. All these pathways are affected by acute brain lesions, such as stroke. Brain-gut communication may therefore impact on the overall outcome after CNS-injury. Until now, contradictory...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-11-01
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| Series: | Frontiers in Neurology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fneur.2018.00937/full |
| _version_ | 1830358405968035840 |
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| author | Naoki Oyama Naoki Oyama Katarzyna Winek Katarzyna Winek Katarzyna Winek Priscilla Bäcker-Koduah Priscilla Bäcker-Koduah Tian Zhang Tian Zhang Claudia Dames Martina Werich Olivia Kershaw Christian Meisel Andreas Meisel Andreas Meisel Andreas Meisel Andreas Meisel Ulrich Dirnagl Ulrich Dirnagl Ulrich Dirnagl Ulrich Dirnagl Ulrich Dirnagl Ulrich Dirnagl |
| author_facet | Naoki Oyama Naoki Oyama Katarzyna Winek Katarzyna Winek Katarzyna Winek Priscilla Bäcker-Koduah Priscilla Bäcker-Koduah Tian Zhang Tian Zhang Claudia Dames Martina Werich Olivia Kershaw Christian Meisel Andreas Meisel Andreas Meisel Andreas Meisel Andreas Meisel Ulrich Dirnagl Ulrich Dirnagl Ulrich Dirnagl Ulrich Dirnagl Ulrich Dirnagl Ulrich Dirnagl |
| author_sort | Naoki Oyama |
| collection | DOAJ |
| description | Background and Purpose: The gut communicates with the brain bidirectionally via neural, humoral and immune pathways. All these pathways are affected by acute brain lesions, such as stroke. Brain-gut communication may therefore impact on the overall outcome after CNS-injury. Until now, contradictory reports on intestinal function and translocation of gut bacteria after experimental stroke have been published. Accordingly, we aimed to specifically investigate the effects of transient focal cerebral ischemia on intestinal permeability, gut associated lymphoid tissue and bacterial translocation in an exploratory study using a well-characterized murine stroke model.Methods: After 60 min of middle cerebral artery occlusion (MCAO) we assessed intestinal morphology (time points after surgery day 0, 3, 5, 14, 21) and tight junction protein expression (occludin and claudin-1 at day 1 and 3) in 12-week-old male C57Bl/6J mice. Lactulose/mannitol/sucralose test was performed to assess intestinal permeability 24–72 h after surgery. To investigate the influence of cerebral ischemia on the local immune system of the gut, main immune cell populations in Peyer's patches (PP) were quantified by flow cytometry. Finally, we evaluated bacterial translocation to extraintestinal organs 24 and 72 h after MCAO by microbiological culture and fluorescence in situ hybridization targeting bacterial 16S rRNA.Results: Transient MCAO decreased claudin-1 expression in the ileum but not in the colon. Intestinal morphology (assessed by light microscopy) and permeability did not change measurably after MCAO. After MCAO, animals had significantly fewer B cells in PP compared to naïve mice.Conclusions: In a murine model of stroke, which leads to large brain infarctions in the middle cerebral artery territory, we did not find evidence for overt alterations neither in gut morphology, barrier proteins and permeability nor presence of intestinal bacterial translocation. |
| first_indexed | 2024-12-20T02:41:38Z |
| format | Article |
| id | doaj.art-9fcf8c7be7084a8eb5233cbc997dba09 |
| institution | Directory Open Access Journal |
| issn | 1664-2295 |
| language | English |
| last_indexed | 2024-12-20T02:41:38Z |
| publishDate | 2018-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Neurology |
| spelling | doaj.art-9fcf8c7be7084a8eb5233cbc997dba092022-12-21T19:56:18ZengFrontiers Media S.A.Frontiers in Neurology1664-22952018-11-01910.3389/fneur.2018.00937411955Exploratory Investigation of Intestinal Function and Bacterial Translocation After Focal Cerebral Ischemia in the MouseNaoki Oyama0Naoki Oyama1Katarzyna Winek2Katarzyna Winek3Katarzyna Winek4Priscilla Bäcker-Koduah5Priscilla Bäcker-Koduah6Tian Zhang7Tian Zhang8Claudia Dames9Martina Werich10Olivia Kershaw11Christian Meisel12Andreas Meisel13Andreas Meisel14Andreas Meisel15Andreas Meisel16Ulrich Dirnagl17Ulrich Dirnagl18Ulrich Dirnagl19Ulrich Dirnagl20Ulrich Dirnagl21Ulrich Dirnagl22Department of Experimental Neurology, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyCenter for Stroke Research Berlin, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyDepartment of Experimental Neurology, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyCenter for Stroke Research Berlin, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyNeurocure Cluster of Excellence, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyDepartment of Experimental Neurology, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyNeurocure Cluster of Excellence, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyDepartment of Experimental Neurology, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyCenter for Stroke Research Berlin, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyInstitute for Medical Immunology, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyMedical Department, Division of Hepatology and Gastroenterology, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyInstitute of Veterinary Pathology, Faculty of Veterinary Medicine, Freie Universität Berlin, Berlin, GermanyInstitute for Medical Immunology, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyDepartment of Experimental Neurology, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyCenter for Stroke Research Berlin, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyNeurocure Cluster of Excellence, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyDepartment of Neurology, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyDepartment of Experimental Neurology, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyCenter for Stroke Research Berlin, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyNeurocure Cluster of Excellence, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyDepartment of Neurology, Charité – Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt Universitäts zu Berlin and Berlin Institute of Health, Berlin, GermanyGerman Center for Neurodegenerative Diseases (DZNE), Partner Site Berlin, Berlin, GermanyQUEST – Center for Transforming Biomedical Research, Berlin Institute of Health, Berlin, GermanyBackground and Purpose: The gut communicates with the brain bidirectionally via neural, humoral and immune pathways. All these pathways are affected by acute brain lesions, such as stroke. Brain-gut communication may therefore impact on the overall outcome after CNS-injury. Until now, contradictory reports on intestinal function and translocation of gut bacteria after experimental stroke have been published. Accordingly, we aimed to specifically investigate the effects of transient focal cerebral ischemia on intestinal permeability, gut associated lymphoid tissue and bacterial translocation in an exploratory study using a well-characterized murine stroke model.Methods: After 60 min of middle cerebral artery occlusion (MCAO) we assessed intestinal morphology (time points after surgery day 0, 3, 5, 14, 21) and tight junction protein expression (occludin and claudin-1 at day 1 and 3) in 12-week-old male C57Bl/6J mice. Lactulose/mannitol/sucralose test was performed to assess intestinal permeability 24–72 h after surgery. To investigate the influence of cerebral ischemia on the local immune system of the gut, main immune cell populations in Peyer's patches (PP) were quantified by flow cytometry. Finally, we evaluated bacterial translocation to extraintestinal organs 24 and 72 h after MCAO by microbiological culture and fluorescence in situ hybridization targeting bacterial 16S rRNA.Results: Transient MCAO decreased claudin-1 expression in the ileum but not in the colon. Intestinal morphology (assessed by light microscopy) and permeability did not change measurably after MCAO. After MCAO, animals had significantly fewer B cells in PP compared to naïve mice.Conclusions: In a murine model of stroke, which leads to large brain infarctions in the middle cerebral artery territory, we did not find evidence for overt alterations neither in gut morphology, barrier proteins and permeability nor presence of intestinal bacterial translocation.https://www.frontiersin.org/article/10.3389/fneur.2018.00937/fullbacterial translocationgut-brain axisgut permeabilitygut associated lymphatic tissueimmune systemstroke |
| spellingShingle | Naoki Oyama Naoki Oyama Katarzyna Winek Katarzyna Winek Katarzyna Winek Priscilla Bäcker-Koduah Priscilla Bäcker-Koduah Tian Zhang Tian Zhang Claudia Dames Martina Werich Olivia Kershaw Christian Meisel Andreas Meisel Andreas Meisel Andreas Meisel Andreas Meisel Ulrich Dirnagl Ulrich Dirnagl Ulrich Dirnagl Ulrich Dirnagl Ulrich Dirnagl Ulrich Dirnagl Exploratory Investigation of Intestinal Function and Bacterial Translocation After Focal Cerebral Ischemia in the Mouse Frontiers in Neurology bacterial translocation gut-brain axis gut permeability gut associated lymphatic tissue immune system stroke |
| title | Exploratory Investigation of Intestinal Function and Bacterial Translocation After Focal Cerebral Ischemia in the Mouse |
| title_full | Exploratory Investigation of Intestinal Function and Bacterial Translocation After Focal Cerebral Ischemia in the Mouse |
| title_fullStr | Exploratory Investigation of Intestinal Function and Bacterial Translocation After Focal Cerebral Ischemia in the Mouse |
| title_full_unstemmed | Exploratory Investigation of Intestinal Function and Bacterial Translocation After Focal Cerebral Ischemia in the Mouse |
| title_short | Exploratory Investigation of Intestinal Function and Bacterial Translocation After Focal Cerebral Ischemia in the Mouse |
| title_sort | exploratory investigation of intestinal function and bacterial translocation after focal cerebral ischemia in the mouse |
| topic | bacterial translocation gut-brain axis gut permeability gut associated lymphatic tissue immune system stroke |
| url | https://www.frontiersin.org/article/10.3389/fneur.2018.00937/full |
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