Histopathological characteristics and coexpression of p53 and p16INK4a proteins in renal cancer
Background/Aim. Renal carcinoma represents histologically heterogeneous group of malignant tumors, with various clinical aggressiveness. The frequency of p53 mutation in primal renal carcinoma is rare, although there are information about its heterogeneous accumulation. The loss of protein p16 expre...
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| Format: | Article |
| Language: | English |
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Military Health Department, Ministry of Defance, Serbia
2008-01-01
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| Series: | Vojnosanitetski Pregled |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2008/0042-84500811820Z.pdf |
| _version_ | 1818364864393904128 |
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| author | Živković Slađana Kostov Miloš Pavlović Svetlana Mijović Žaklina |
| author_facet | Živković Slađana Kostov Miloš Pavlović Svetlana Mijović Žaklina |
| author_sort | Živković Slađana |
| collection | DOAJ |
| description | Background/Aim. Renal carcinoma represents histologically heterogeneous group of malignant tumors, with various clinical aggressiveness. The frequency of p53 mutation in primal renal carcinoma is rare, although there are information about its heterogeneous accumulation. The loss of protein p16 expression in primal renal carcinoma is detected in 20-30% of the cases. The aim of this paper was to determine frequency of mutated protein p53 and expression of protein p16INK4a in renal carcinoma, to analyze their correlative relation and relation with the examined clinicopathological parameters. Methods. The examination included 12 patients (66.7% men, 33.3% women), with patohistologically verified renal carcinoma. Expression of mutated form of protein p53 and protein p16 was determined in tissue samples, by immunohistochemical analysis using of mice monoclonical antibodies produced by DAKO, Denmark. Results. In 9 (75%) of the cases was detected mutated protein p53, of whom 66.6% had higher histological gradus of tumor (G3-4) and higher pathological stadium of the disease (pT3a-b) at the same time. In 7 (58.3%) and 5 (41.7%) of the cases expression of protein p16, the loss of expression of protein p16 were detected respectively. A statistically significant positive correlation was determined between pathological stadium of disease (TNM) and the degree of tumor differentiation (G) (ρ = 0.834; p < 0.001), as well as between TNM and mitotic index (ρ = 0.622; p = 0.031). Conclusion. A mutated form of protein p53 exists in 75% of the cases with the renal carcinoma and 66.6% of then have higher histological gradus of tumor and higher stadium of tumor disease at the same time. Coexpression of mutated protein p53 and protein p16INK4a in renal carcinoma is not statistically significant and it is not in correlation with clinicopathological parameters. Immunohistochemical analysis of mutated protein p53 in renal carcinoma can have predictive significance. |
| first_indexed | 2024-12-13T22:11:09Z |
| format | Article |
| id | doaj.art-9fd144c3420c4e0a9faa7a4ec7cc2f63 |
| institution | Directory Open Access Journal |
| issn | 0042-8450 |
| language | English |
| last_indexed | 2024-12-13T22:11:09Z |
| publishDate | 2008-01-01 |
| publisher | Military Health Department, Ministry of Defance, Serbia |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj.art-9fd144c3420c4e0a9faa7a4ec7cc2f632022-12-21T23:29:42ZengMilitary Health Department, Ministry of Defance, SerbiaVojnosanitetski Pregled0042-84502008-01-01651182082410.2298/VSP0811820ZHistopathological characteristics and coexpression of p53 and p16INK4a proteins in renal cancerŽivković SlađanaKostov MilošPavlović SvetlanaMijović ŽaklinaBackground/Aim. Renal carcinoma represents histologically heterogeneous group of malignant tumors, with various clinical aggressiveness. The frequency of p53 mutation in primal renal carcinoma is rare, although there are information about its heterogeneous accumulation. The loss of protein p16 expression in primal renal carcinoma is detected in 20-30% of the cases. The aim of this paper was to determine frequency of mutated protein p53 and expression of protein p16INK4a in renal carcinoma, to analyze their correlative relation and relation with the examined clinicopathological parameters. Methods. The examination included 12 patients (66.7% men, 33.3% women), with patohistologically verified renal carcinoma. Expression of mutated form of protein p53 and protein p16 was determined in tissue samples, by immunohistochemical analysis using of mice monoclonical antibodies produced by DAKO, Denmark. Results. In 9 (75%) of the cases was detected mutated protein p53, of whom 66.6% had higher histological gradus of tumor (G3-4) and higher pathological stadium of the disease (pT3a-b) at the same time. In 7 (58.3%) and 5 (41.7%) of the cases expression of protein p16, the loss of expression of protein p16 were detected respectively. A statistically significant positive correlation was determined between pathological stadium of disease (TNM) and the degree of tumor differentiation (G) (ρ = 0.834; p < 0.001), as well as between TNM and mitotic index (ρ = 0.622; p = 0.031). Conclusion. A mutated form of protein p53 exists in 75% of the cases with the renal carcinoma and 66.6% of then have higher histological gradus of tumor and higher stadium of tumor disease at the same time. Coexpression of mutated protein p53 and protein p16INK4a in renal carcinoma is not statistically significant and it is not in correlation with clinicopathological parameters. Immunohistochemical analysis of mutated protein p53 in renal carcinoma can have predictive significance.http://www.doiserbia.nb.rs/img/doi/0042-8450/2008/0042-84500811820Z.pdfcarcinomarenal cellstumor suppressor protein p53cyclin dependent kinase inhibitor p16immunohistochemistry |
| spellingShingle | Živković Slađana Kostov Miloš Pavlović Svetlana Mijović Žaklina Histopathological characteristics and coexpression of p53 and p16INK4a proteins in renal cancer Vojnosanitetski Pregled carcinoma renal cells tumor suppressor protein p53 cyclin dependent kinase inhibitor p16 immunohistochemistry |
| title | Histopathological characteristics and coexpression of p53 and p16INK4a proteins in renal cancer |
| title_full | Histopathological characteristics and coexpression of p53 and p16INK4a proteins in renal cancer |
| title_fullStr | Histopathological characteristics and coexpression of p53 and p16INK4a proteins in renal cancer |
| title_full_unstemmed | Histopathological characteristics and coexpression of p53 and p16INK4a proteins in renal cancer |
| title_short | Histopathological characteristics and coexpression of p53 and p16INK4a proteins in renal cancer |
| title_sort | histopathological characteristics and coexpression of p53 and p16ink4a proteins in renal cancer |
| topic | carcinoma renal cells tumor suppressor protein p53 cyclin dependent kinase inhibitor p16 immunohistochemistry |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2008/0042-84500811820Z.pdf |
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