Effect of fluorophenylalanine on indole-3-acetic acid oxidation in Avena coleoptiles
Effects of the amino acid analog D,L-p-fluorophenylalanine on auxin oxidizing activity from Avena saliva cv. Clintford have been examined. Incubation of etiolated Avena coleoptile apices with D,L-p-fluorophenylalanine, previously reported to promote elongation and depress phenolic metabolism, lowers...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2014-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/1187 |
| _version_ | 1818191121459707904 |
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| author | Andrew B. Maksymowych Joseph A.J. Orkwiszewski |
| author_facet | Andrew B. Maksymowych Joseph A.J. Orkwiszewski |
| author_sort | Andrew B. Maksymowych |
| collection | DOAJ |
| description | Effects of the amino acid analog D,L-p-fluorophenylalanine on auxin oxidizing activity from Avena saliva cv. Clintford have been examined. Incubation of etiolated Avena coleoptile apices with D,L-p-fluorophenylalanine, previously reported to promote elongation and depress phenolic metabolism, lowers auxin oxidizing activity. Temperature and pH profiles demonstrated that depression of oxidizing activity by D,L-p-fluorophenylalanine occurs over a relatively wide range. Incubation of coleoptiles in media containing L-phenylalanine alone or L-phenylalanine combined with D,L-p-fluorophenylalanine result in oxidizing activities which are, respectively, slightly higher than or restored to control levels. Thus, dark incubation of coleoptiles with D,L-p-fluorophenylalanine results in auxin oxidizing activity which differs from that of control and other treatment groups. Oxidizing activity from D,L-p-fluorophenylalanine pretreated coleoptiles has a Michaelis constant (70 µM) and a maximum velocity (0.200) which are consistently lower than the control constants. These findings provide further clarification of the effects of D,L-p-fluorophenylalanine, on coleoptile elongation and auxin oxidizing activity in etiolated Avena coleoptiles. |
| first_indexed | 2024-12-12T00:09:34Z |
| format | Article |
| id | doaj.art-9fd4bf2628b14b9cb088e308cfb0858d |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-12T00:09:34Z |
| publishDate | 2014-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-9fd4bf2628b14b9cb088e308cfb0858d2022-12-22T00:45:01ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802014-01-01613-438939710.5586/asbp.1992.034923Effect of fluorophenylalanine on indole-3-acetic acid oxidation in Avena coleoptilesAndrew B. Maksymowych0Joseph A.J. Orkwiszewski1Bryn Mawr CollegeVillanova UniversityEffects of the amino acid analog D,L-p-fluorophenylalanine on auxin oxidizing activity from Avena saliva cv. Clintford have been examined. Incubation of etiolated Avena coleoptile apices with D,L-p-fluorophenylalanine, previously reported to promote elongation and depress phenolic metabolism, lowers auxin oxidizing activity. Temperature and pH profiles demonstrated that depression of oxidizing activity by D,L-p-fluorophenylalanine occurs over a relatively wide range. Incubation of coleoptiles in media containing L-phenylalanine alone or L-phenylalanine combined with D,L-p-fluorophenylalanine result in oxidizing activities which are, respectively, slightly higher than or restored to control levels. Thus, dark incubation of coleoptiles with D,L-p-fluorophenylalanine results in auxin oxidizing activity which differs from that of control and other treatment groups. Oxidizing activity from D,L-p-fluorophenylalanine pretreated coleoptiles has a Michaelis constant (70 µM) and a maximum velocity (0.200) which are consistently lower than the control constants. These findings provide further clarification of the effects of D,L-p-fluorophenylalanine, on coleoptile elongation and auxin oxidizing activity in etiolated Avena coleoptiles.https://pbsociety.org.pl/journals/index.php/asbp/article/view/1187auxin oxidationAvena coleoptilefluorophenylalaninephenolic metabolism |
| spellingShingle | Andrew B. Maksymowych Joseph A.J. Orkwiszewski Effect of fluorophenylalanine on indole-3-acetic acid oxidation in Avena coleoptiles Acta Societatis Botanicorum Poloniae auxin oxidation Avena coleoptile fluorophenylalanine phenolic metabolism |
| title | Effect of fluorophenylalanine on indole-3-acetic acid oxidation in Avena coleoptiles |
| title_full | Effect of fluorophenylalanine on indole-3-acetic acid oxidation in Avena coleoptiles |
| title_fullStr | Effect of fluorophenylalanine on indole-3-acetic acid oxidation in Avena coleoptiles |
| title_full_unstemmed | Effect of fluorophenylalanine on indole-3-acetic acid oxidation in Avena coleoptiles |
| title_short | Effect of fluorophenylalanine on indole-3-acetic acid oxidation in Avena coleoptiles |
| title_sort | effect of fluorophenylalanine on indole 3 acetic acid oxidation in avena coleoptiles |
| topic | auxin oxidation Avena coleoptile fluorophenylalanine phenolic metabolism |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/1187 |
| work_keys_str_mv | AT andrewbmaksymowych effectoffluorophenylalanineonindole3aceticacidoxidationinavenacoleoptiles AT josephajorkwiszewski effectoffluorophenylalanineonindole3aceticacidoxidationinavenacoleoptiles |