Identification and Characterization of Bovine Viral Diarrhea Virus from Indonesian Cattle (IDENTIFIKASI DAN KARAKTERISASI VIRUS BOVINE VIRAL DIARRHEA DARI SAPI INDONESIA)
Bovine viral diarrhea virus (BVDV) is an important viral disease, which a ubiquitous pathogen ofcattle with worldwide economic importance and due to its misdiagnose with other viruses. The goal of thecurrent study was to identify and characterize of BVDV by reverse transcriptase polymerase chainreac...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Universitas Udayana
2015-05-01
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| Series: | Jurnal Veteriner |
| Subjects: | |
| Online Access: | https://ojs.unud.ac.id/index.php/jvet/article/view/13267 |
| _version_ | 1811239905855012864 |
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| author | Muharam Saepulloh Indrawati Sendow |
| author_facet | Muharam Saepulloh Indrawati Sendow |
| author_sort | Muharam Saepulloh |
| collection | DOAJ |
| description | Bovine viral diarrhea virus (BVDV) is an important viral disease, which a ubiquitous pathogen ofcattle with worldwide economic importance and due to its misdiagnose with other viruses. The goal of thecurrent study was to identify and characterize of BVDV by reverse transcriptase polymerase chainreaction (RT-PCR) and followed by sequence genome analyses. Blood, feces, and semen samples werecollected from 588 selected cattle from animals suffering from diarrhea and respiratory manifestation. RTPCRresults showed that the 69 (11.74%) samples were positive to BVDV. Further molecularcharacterization was conducted only with 17 PCR positive samples. The results indicated the 17 IndonesianBVD virus isolates were belonging to the genotype-1 of BVDV (BVDV-1) based on sequence analysis anda phylogenetic relationship between Indonesian BVDV isolates and BVDV in the world. This finding is thefirst report of BVD-1 circulated in Indonesian cattle. |
| first_indexed | 2024-04-12T13:11:11Z |
| format | Article |
| id | doaj.art-9fd59c20ba624212a1105491760a773a |
| institution | Directory Open Access Journal |
| issn | 1411-8327 2477-5665 |
| language | English |
| last_indexed | 2024-04-12T13:11:11Z |
| publishDate | 2015-05-01 |
| publisher | Universitas Udayana |
| record_format | Article |
| series | Jurnal Veteriner |
| spelling | doaj.art-9fd59c20ba624212a1105491760a773a2022-12-22T03:31:53ZengUniversitas UdayanaJurnal Veteriner1411-83272477-56652015-05-011611713267Identification and Characterization of Bovine Viral Diarrhea Virus from Indonesian Cattle (IDENTIFIKASI DAN KARAKTERISASI VIRUS BOVINE VIRAL DIARRHEA DARI SAPI INDONESIA)Muharam Saepulloh0Indrawati SendowBagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, BaliBovine viral diarrhea virus (BVDV) is an important viral disease, which a ubiquitous pathogen ofcattle with worldwide economic importance and due to its misdiagnose with other viruses. The goal of thecurrent study was to identify and characterize of BVDV by reverse transcriptase polymerase chainreaction (RT-PCR) and followed by sequence genome analyses. Blood, feces, and semen samples werecollected from 588 selected cattle from animals suffering from diarrhea and respiratory manifestation. RTPCRresults showed that the 69 (11.74%) samples were positive to BVDV. Further molecularcharacterization was conducted only with 17 PCR positive samples. The results indicated the 17 IndonesianBVD virus isolates were belonging to the genotype-1 of BVDV (BVDV-1) based on sequence analysis anda phylogenetic relationship between Indonesian BVDV isolates and BVDV in the world. This finding is thefirst report of BVD-1 circulated in Indonesian cattle.https://ojs.unud.ac.id/index.php/jvet/article/view/13267BVDV, RT-PCR, genotype-1 |
| spellingShingle | Muharam Saepulloh Indrawati Sendow Identification and Characterization of Bovine Viral Diarrhea Virus from Indonesian Cattle (IDENTIFIKASI DAN KARAKTERISASI VIRUS BOVINE VIRAL DIARRHEA DARI SAPI INDONESIA) Jurnal Veteriner BVDV, RT-PCR, genotype-1 |
| title | Identification and Characterization of Bovine Viral Diarrhea Virus from Indonesian Cattle (IDENTIFIKASI DAN KARAKTERISASI VIRUS BOVINE VIRAL DIARRHEA DARI SAPI INDONESIA) |
| title_full | Identification and Characterization of Bovine Viral Diarrhea Virus from Indonesian Cattle (IDENTIFIKASI DAN KARAKTERISASI VIRUS BOVINE VIRAL DIARRHEA DARI SAPI INDONESIA) |
| title_fullStr | Identification and Characterization of Bovine Viral Diarrhea Virus from Indonesian Cattle (IDENTIFIKASI DAN KARAKTERISASI VIRUS BOVINE VIRAL DIARRHEA DARI SAPI INDONESIA) |
| title_full_unstemmed | Identification and Characterization of Bovine Viral Diarrhea Virus from Indonesian Cattle (IDENTIFIKASI DAN KARAKTERISASI VIRUS BOVINE VIRAL DIARRHEA DARI SAPI INDONESIA) |
| title_short | Identification and Characterization of Bovine Viral Diarrhea Virus from Indonesian Cattle (IDENTIFIKASI DAN KARAKTERISASI VIRUS BOVINE VIRAL DIARRHEA DARI SAPI INDONESIA) |
| title_sort | identification and characterization of bovine viral diarrhea virus from indonesian cattle identifikasi dan karakterisasi virus bovine viral diarrhea dari sapi indonesia |
| topic | BVDV, RT-PCR, genotype-1 |
| url | https://ojs.unud.ac.id/index.php/jvet/article/view/13267 |
| work_keys_str_mv | AT muharamsaepulloh identificationandcharacterizationofbovineviraldiarrheavirusfromindonesiancattleidentifikasidankarakterisasivirusbovineviraldiarrheadarisapiindonesia AT indrawatisendow identificationandcharacterizationofbovineviraldiarrheavirusfromindonesiancattleidentifikasidankarakterisasivirusbovineviraldiarrheadarisapiindonesia |