Protective Effect of Resveratrol against Hypoxia-Induced Neural Oxidative Stress
Oxidative stress plays an important role in brain aging and in neurodegenerative diseases. New therapeutic agents are necessary to cross the blood–brain barrier and target disease pathogenesis without causing disagreeable side effects. Resveratrol (RSV) may act as a neuroprotective compound, but lit...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Journal of Personalized Medicine |
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| Online Access: | https://www.mdpi.com/2075-4426/12/8/1202 |
| _version_ | 1797409486926774272 |
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| author | Amogh Auti Nicola Alessio Andrea Ballini Mario Dioguardi Stefania Cantore Salvatore Scacco Antonio Vitiello Lucio Quagliuolo Barbara Rinaldi Luigi Santacroce Marina Di Domenico Mariarosaria Boccellino |
| author_facet | Amogh Auti Nicola Alessio Andrea Ballini Mario Dioguardi Stefania Cantore Salvatore Scacco Antonio Vitiello Lucio Quagliuolo Barbara Rinaldi Luigi Santacroce Marina Di Domenico Mariarosaria Boccellino |
| author_sort | Amogh Auti |
| collection | DOAJ |
| description | Oxidative stress plays an important role in brain aging and in neurodegenerative diseases. New therapeutic agents are necessary to cross the blood–brain barrier and target disease pathogenesis without causing disagreeable side effects. Resveratrol (RSV) may act as a neuroprotective compound, but little is known about its potential in improving the cognitive and metabolic aspects that are associated with neurodegenerative diseases. The objective of this study was to investigate the protective effects and the underlying mechanisms of RSV against hypoxia-induced oxidative stress in neuronal PC12 cells. For the induction of the hypoxia model, the cells were exposed to oxygen-deprived gas in a hypoxic chamber. Cell cycle and apoptosis were analyzed by a fluorescence activated cell sorting (FACS) analysis. The intracellular reactive oxygen species (ROS) level was analyzed by using dichlorodihydrofluorescein diacetate (DCFDA) and 5-(and-6)-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA) tests. The expression of activated caspase-3, -9, Bcl-2, Bax, p53, and SOD was investigated by a Western blot analysis. We found that hypoxia reduced PC12 viability by inducing apoptosis, while RSV treatment attenuated the ROS-induced damage by reducing caspase-3, -9, and the Bax/Bcl-2 ratio. The RSV treated groups were found to improve cellular health, with a 7.41% increase in the S phase population in the 10 µM group, compared to the control. Hence, RSV has a protective effect in neuronal cells and may halt the cell cycle in the G1/S phase to repair the intracellular damage. Therefore, RSV could be a good candidate to act as an antioxidant and promising preventive therapeutic agent in neurodegenerative diseases for personalized medicine. |
| first_indexed | 2024-03-09T04:15:15Z |
| format | Article |
| id | doaj.art-9fd62bcbabe04ad89cee34d8449aef37 |
| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-09T04:15:15Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Personalized Medicine |
| spelling | doaj.art-9fd62bcbabe04ad89cee34d8449aef372023-12-03T13:55:33ZengMDPI AGJournal of Personalized Medicine2075-44262022-07-01128120210.3390/jpm12081202Protective Effect of Resveratrol against Hypoxia-Induced Neural Oxidative StressAmogh Auti0Nicola Alessio1Andrea Ballini2Mario Dioguardi3Stefania Cantore4Salvatore Scacco5Antonio Vitiello6Lucio Quagliuolo7Barbara Rinaldi8Luigi Santacroce9Marina Di Domenico10Mariarosaria Boccellino11Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Precision Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalyIndependent Researcher, 70129 Bari, ItalyDepartment of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari ALDO MORO, 70124 Bari, ItalyPharmaceutical Department, Local Sanitary Unit Umbria 1, 06132 Perugia, ItalyDepartment of Precision Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyMicrobiology and Virology Unit, Department of Interdisciplinary Medicine, School of Medicine, University of Bari ALDO MORO, 70124 Bari, ItalyDepartment of Precision Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Precision Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyOxidative stress plays an important role in brain aging and in neurodegenerative diseases. New therapeutic agents are necessary to cross the blood–brain barrier and target disease pathogenesis without causing disagreeable side effects. Resveratrol (RSV) may act as a neuroprotective compound, but little is known about its potential in improving the cognitive and metabolic aspects that are associated with neurodegenerative diseases. The objective of this study was to investigate the protective effects and the underlying mechanisms of RSV against hypoxia-induced oxidative stress in neuronal PC12 cells. For the induction of the hypoxia model, the cells were exposed to oxygen-deprived gas in a hypoxic chamber. Cell cycle and apoptosis were analyzed by a fluorescence activated cell sorting (FACS) analysis. The intracellular reactive oxygen species (ROS) level was analyzed by using dichlorodihydrofluorescein diacetate (DCFDA) and 5-(and-6)-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA) tests. The expression of activated caspase-3, -9, Bcl-2, Bax, p53, and SOD was investigated by a Western blot analysis. We found that hypoxia reduced PC12 viability by inducing apoptosis, while RSV treatment attenuated the ROS-induced damage by reducing caspase-3, -9, and the Bax/Bcl-2 ratio. The RSV treated groups were found to improve cellular health, with a 7.41% increase in the S phase population in the 10 µM group, compared to the control. Hence, RSV has a protective effect in neuronal cells and may halt the cell cycle in the G1/S phase to repair the intracellular damage. Therefore, RSV could be a good candidate to act as an antioxidant and promising preventive therapeutic agent in neurodegenerative diseases for personalized medicine.https://www.mdpi.com/2075-4426/12/8/1202PC12 cellsresveratroloxidative stressischemiahypoxiapersonalized medicine |
| spellingShingle | Amogh Auti Nicola Alessio Andrea Ballini Mario Dioguardi Stefania Cantore Salvatore Scacco Antonio Vitiello Lucio Quagliuolo Barbara Rinaldi Luigi Santacroce Marina Di Domenico Mariarosaria Boccellino Protective Effect of Resveratrol against Hypoxia-Induced Neural Oxidative Stress Journal of Personalized Medicine PC12 cells resveratrol oxidative stress ischemia hypoxia personalized medicine |
| title | Protective Effect of Resveratrol against Hypoxia-Induced Neural Oxidative Stress |
| title_full | Protective Effect of Resveratrol against Hypoxia-Induced Neural Oxidative Stress |
| title_fullStr | Protective Effect of Resveratrol against Hypoxia-Induced Neural Oxidative Stress |
| title_full_unstemmed | Protective Effect of Resveratrol against Hypoxia-Induced Neural Oxidative Stress |
| title_short | Protective Effect of Resveratrol against Hypoxia-Induced Neural Oxidative Stress |
| title_sort | protective effect of resveratrol against hypoxia induced neural oxidative stress |
| topic | PC12 cells resveratrol oxidative stress ischemia hypoxia personalized medicine |
| url | https://www.mdpi.com/2075-4426/12/8/1202 |
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