Anti-hyperalgesic effect of Mangifera indica L. extract on the sciatic chronic constriction injury model in rats
Context: The aqueous extract of Mangifera indica L. stem bark (MSBE) shows antioxidant, anti-inflammatory and analgesic properties. Aims: To test the MSBE in chronic constriction injury (CCI) of the sciatic nerve in rats, a classical model of neuropathic pain. Methods: Given the possibility that s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2014-04-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | http://jppres.com/jppres/pdf/vol2/jppres14.017_2.2.36.pdf |
| _version_ | 1818350522914045952 |
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| author | Bárbara B. Garrido-Suárez Marian Castro Labrada Nelson Merino Odalys Valdés René Delgado-Hernández |
| author_facet | Bárbara B. Garrido-Suárez Marian Castro Labrada Nelson Merino Odalys Valdés René Delgado-Hernández |
| author_sort | Bárbara B. Garrido-Suárez |
| collection | DOAJ |
| description | Context: The aqueous extract of Mangifera indica L. stem bark (MSBE) shows antioxidant, anti-inflammatory and analgesic properties.
Aims: To test the MSBE in chronic constriction injury (CCI) of the sciatic nerve in rats, a classical model of neuropathic pain.
Methods: Given the possibility that some clinical effect of MSBE can appear only after its chronic administration, we designed a long term medication protocol with 500 mg/kg, p.o. or distilled water daily during 8 days from 9 days post-CCI. Pregabalin (10 mg/kg, p.o.) was used as reference drug. Sham CCI animals received vehicle. Behavioral tests were carried out before CCI, at 9 and 16 days after injury. A section of sciatic nerve, 5 mm distal to the ligature site was dissected for histopathological studies. A single oral similar dose or vehicle was administered to mononeuropathic rats, 14 days after surgery. Mechano-hyperalgesia and thermal hyperalgesia of the ipsilateral paw were determined using a modification of pin prick method and the unilateral hot plate, respectively before CCI, 0, 30, 60, 120 and 180 min post-administration.
Results: Repeated oral MSBE doses reduced nociceptive score, increased paw withdrawal latency and attenuated CCI-induced Wallerian degeneration-related changes involved in the hyperalgesic state of CCI rats. Likewise, MSBE shows significant mechanical and thermal anti-hyperalgesic effect from 1h after its single administration.
Conclusions: The study of MSBE should be focused in neuropathic pain models since this natural product could have a clinical relevance in the treatment of neuropathic pain syndromes. |
| first_indexed | 2024-12-13T18:23:11Z |
| format | Article |
| id | doaj.art-9fd6f1e9752c4950bd8b0ca43826063c |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-12-13T18:23:11Z |
| publishDate | 2014-04-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-9fd6f1e9752c4950bd8b0ca43826063c2022-12-21T23:35:40ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502014-04-01223644Anti-hyperalgesic effect of Mangifera indica L. extract on the sciatic chronic constriction injury model in ratsBárbara B. Garrido-SuárezMarian Castro LabradaNelson MerinoOdalys ValdésRené Delgado-HernándezContext: The aqueous extract of Mangifera indica L. stem bark (MSBE) shows antioxidant, anti-inflammatory and analgesic properties. Aims: To test the MSBE in chronic constriction injury (CCI) of the sciatic nerve in rats, a classical model of neuropathic pain. Methods: Given the possibility that some clinical effect of MSBE can appear only after its chronic administration, we designed a long term medication protocol with 500 mg/kg, p.o. or distilled water daily during 8 days from 9 days post-CCI. Pregabalin (10 mg/kg, p.o.) was used as reference drug. Sham CCI animals received vehicle. Behavioral tests were carried out before CCI, at 9 and 16 days after injury. A section of sciatic nerve, 5 mm distal to the ligature site was dissected for histopathological studies. A single oral similar dose or vehicle was administered to mononeuropathic rats, 14 days after surgery. Mechano-hyperalgesia and thermal hyperalgesia of the ipsilateral paw were determined using a modification of pin prick method and the unilateral hot plate, respectively before CCI, 0, 30, 60, 120 and 180 min post-administration. Results: Repeated oral MSBE doses reduced nociceptive score, increased paw withdrawal latency and attenuated CCI-induced Wallerian degeneration-related changes involved in the hyperalgesic state of CCI rats. Likewise, MSBE shows significant mechanical and thermal anti-hyperalgesic effect from 1h after its single administration. Conclusions: The study of MSBE should be focused in neuropathic pain models since this natural product could have a clinical relevance in the treatment of neuropathic pain syndromes.http://jppres.com/jppres/pdf/vol2/jppres14.017_2.2.36.pdfHyperalgesiamangiferinneuropathic painDolor neuropáticohiperalgesiamangiferina |
| spellingShingle | Bárbara B. Garrido-Suárez Marian Castro Labrada Nelson Merino Odalys Valdés René Delgado-Hernández Anti-hyperalgesic effect of Mangifera indica L. extract on the sciatic chronic constriction injury model in rats Journal of Pharmacy & Pharmacognosy Research Hyperalgesia mangiferin neuropathic pain Dolor neuropático hiperalgesia mangiferina |
| title | Anti-hyperalgesic effect of Mangifera indica L. extract on the sciatic chronic constriction injury model in rats |
| title_full | Anti-hyperalgesic effect of Mangifera indica L. extract on the sciatic chronic constriction injury model in rats |
| title_fullStr | Anti-hyperalgesic effect of Mangifera indica L. extract on the sciatic chronic constriction injury model in rats |
| title_full_unstemmed | Anti-hyperalgesic effect of Mangifera indica L. extract on the sciatic chronic constriction injury model in rats |
| title_short | Anti-hyperalgesic effect of Mangifera indica L. extract on the sciatic chronic constriction injury model in rats |
| title_sort | anti hyperalgesic effect of mangifera indica l extract on the sciatic chronic constriction injury model in rats |
| topic | Hyperalgesia mangiferin neuropathic pain Dolor neuropático hiperalgesia mangiferina |
| url | http://jppres.com/jppres/pdf/vol2/jppres14.017_2.2.36.pdf |
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