Effect of Thalidomide on the MMP-2 Protein Expression and Mast Cells in the Lung Fibrosis Induced by Bleomycin in Mice
Introduction and AimsMatrix metallo proteinase-2 (MMP-2) is a family of proteolytic enzymes involved in degrading and remodeling the extracellular matrix and basement membrane. Researchers have shown that Mast cells have an important role in the inflammatory disease including lung fibrosis and they...
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| Format: | Article |
| Language: | fas |
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Neyshabur University of Medical Sciences
2015-02-01
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| Series: | Majallah-i Dānishkadah-i ̒ulūm-i Pizishkī-i Niyshābūr |
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| Online Access: | http://journal.nums.ac.ir/browse.php?a_code=A-10-1-39&slc_lang=en&sid=1 |
| _version_ | 1819212720243736576 |
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| author | Ghazal Keshavarz Mohammad Jafar Rezaie |
| author_facet | Ghazal Keshavarz Mohammad Jafar Rezaie |
| author_sort | Ghazal Keshavarz |
| collection | DOAJ |
| description | Introduction and AimsMatrix metallo proteinase-2 (MMP-2) is a family of proteolytic enzymes involved in degrading and remodeling the extracellular
matrix and basement membrane. Researchers have shown that Mast cells have an important role in the inflammatory
disease including lung fibrosis and they can be as the source of MMP-2 protein. The aim of this research is the examination
of Thalidomide effect on the MMP-2 protein expression and Mast cells in the lung fibrosis induced by Bleomycin in mice.Materials and MethodsIn this research, 32 male adult C57BL/6 mices were randomly divided into 4 groups. Mices received in group 1 (Bleomycin)
Bleomycin sulfate, in group 2 (Bleomycin+Thalidomide) Bleomycin beside Thalidomide, in group 3(Thalidomide) Thalidomide
and in group 4 (Carboxy Methyl Cellulose) Carboxy Methyl Cellulose via intraperitoneum. At the end of experiment,
mice’s lung samples were prepared and histological and immunohistochemical studies were performed about them. After
the investigation of tissue slides, number of MMP-2 protein expression cells and Mast cells were calculated and results were
analyzed by using OneWay ANOVA and Tukey’s test.ResultsHistological and immunohistochemical studies showed a significant increase in the number of MMP-2 protein expression
and Mast cells in the Bleomycin group in comparison with the Carboxy Methyl Cellulose group (p < 0.001). But number
of these cells in the Bleomycin+Thalidomide group decreased significantly compared to the Bleomycin group (p < 0.001).ConclusionThe results showed that the number of expressive cells of MMP-2 protein and Mast cells decreases by Thalidomide and
subsequently reduces lung fibrosis in the mice.* Corresponding Author: Kurdistan University of Medical Science, Department of Anatomical Sciences.Email: Rezaiemjafar@gmail.com |
| first_indexed | 2024-12-23T06:47:27Z |
| format | Article |
| id | doaj.art-9fd78afccf464e1fb18f8d260b8e209f |
| institution | Directory Open Access Journal |
| issn | 2383-3203 2476-2768 |
| language | fas |
| last_indexed | 2024-12-23T06:47:27Z |
| publishDate | 2015-02-01 |
| publisher | Neyshabur University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishkadah-i ̒ulūm-i Pizishkī-i Niyshābūr |
| spelling | doaj.art-9fd78afccf464e1fb18f8d260b8e209f2022-12-21T17:56:32ZfasNeyshabur University of Medical SciencesMajallah-i Dānishkadah-i ̒ulūm-i Pizishkī-i Niyshābūr2383-32032476-27682015-02-01251221Effect of Thalidomide on the MMP-2 Protein Expression and Mast Cells in the Lung Fibrosis Induced by Bleomycin in MiceGhazal Keshavarz0Mohammad Jafar Rezaie1 Introduction and AimsMatrix metallo proteinase-2 (MMP-2) is a family of proteolytic enzymes involved in degrading and remodeling the extracellular matrix and basement membrane. Researchers have shown that Mast cells have an important role in the inflammatory disease including lung fibrosis and they can be as the source of MMP-2 protein. The aim of this research is the examination of Thalidomide effect on the MMP-2 protein expression and Mast cells in the lung fibrosis induced by Bleomycin in mice.Materials and MethodsIn this research, 32 male adult C57BL/6 mices were randomly divided into 4 groups. Mices received in group 1 (Bleomycin) Bleomycin sulfate, in group 2 (Bleomycin+Thalidomide) Bleomycin beside Thalidomide, in group 3(Thalidomide) Thalidomide and in group 4 (Carboxy Methyl Cellulose) Carboxy Methyl Cellulose via intraperitoneum. At the end of experiment, mice’s lung samples were prepared and histological and immunohistochemical studies were performed about them. After the investigation of tissue slides, number of MMP-2 protein expression cells and Mast cells were calculated and results were analyzed by using OneWay ANOVA and Tukey’s test.ResultsHistological and immunohistochemical studies showed a significant increase in the number of MMP-2 protein expression and Mast cells in the Bleomycin group in comparison with the Carboxy Methyl Cellulose group (p < 0.001). But number of these cells in the Bleomycin+Thalidomide group decreased significantly compared to the Bleomycin group (p < 0.001).ConclusionThe results showed that the number of expressive cells of MMP-2 protein and Mast cells decreases by Thalidomide and subsequently reduces lung fibrosis in the mice.* Corresponding Author: Kurdistan University of Medical Science, Department of Anatomical Sciences.Email: Rezaiemjafar@gmail.comhttp://journal.nums.ac.ir/browse.php?a_code=A-10-1-39&slc_lang=en&sid=1Lung Fibrosis MMP-2 Mast Cells Thalidomide |
| spellingShingle | Ghazal Keshavarz Mohammad Jafar Rezaie Effect of Thalidomide on the MMP-2 Protein Expression and Mast Cells in the Lung Fibrosis Induced by Bleomycin in Mice Majallah-i Dānishkadah-i ̒ulūm-i Pizishkī-i Niyshābūr Lung Fibrosis MMP-2 Mast Cells Thalidomide |
| title | Effect of Thalidomide on the MMP-2 Protein Expression and Mast Cells in the Lung Fibrosis Induced by Bleomycin in Mice |
| title_full | Effect of Thalidomide on the MMP-2 Protein Expression and Mast Cells in the Lung Fibrosis Induced by Bleomycin in Mice |
| title_fullStr | Effect of Thalidomide on the MMP-2 Protein Expression and Mast Cells in the Lung Fibrosis Induced by Bleomycin in Mice |
| title_full_unstemmed | Effect of Thalidomide on the MMP-2 Protein Expression and Mast Cells in the Lung Fibrosis Induced by Bleomycin in Mice |
| title_short | Effect of Thalidomide on the MMP-2 Protein Expression and Mast Cells in the Lung Fibrosis Induced by Bleomycin in Mice |
| title_sort | effect of thalidomide on the mmp 2 protein expression and mast cells in the lung fibrosis induced by bleomycin in mice |
| topic | Lung Fibrosis MMP-2 Mast Cells Thalidomide |
| url | http://journal.nums.ac.ir/browse.php?a_code=A-10-1-39&slc_lang=en&sid=1 |
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