Comparative Analysis of Re-Annotated Genes Provides Insight into Evolutionary Divergence and Expressions of Aquaporin Family in Pepper
Aquaporins (AQPs) are known to have a vital role in water transport in all living organisms including agriculturally important crops, but a comprehensive genomic study of AQPs in pepper has not been implemented. Here, we updated previous gene annotations and generated a total of 259 AQP genes from f...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/6/1039 |
| _version_ | 1797533154558345216 |
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| author | Yeon Mi Lee Geun Young Chae Min Kyung Kim Seungill Kim |
| author_facet | Yeon Mi Lee Geun Young Chae Min Kyung Kim Seungill Kim |
| author_sort | Yeon Mi Lee |
| collection | DOAJ |
| description | Aquaporins (AQPs) are known to have a vital role in water transport in all living organisms including agriculturally important crops, but a comprehensive genomic study of AQPs in pepper has not been implemented. Here, we updated previous gene annotations and generated a total of 259 AQP genes from five plants, including pepper. Phylogenetic and motif analyses revealed that a large proportion of pepper AQP genes belong to the specific subgroup of tonoplast intrinsic protein (TIP) subfamily, TIP4. Chromosomal localization and estimated duplication times illustrated that genes in TIP4 formed a tandem array on the short arm of chromosome 1, resulting from pepper-specific expansion after its divergence with <i>Solanaceae</i> species. Transcriptome analyses under various abiotic stress conditions revealed that transport-, photosystem-, and thylakoid-related genes were generally enriched in expression clusters containing AQP genes in pepper. These results provide valuable genomic resources and insight into the evolutionary mechanism that generate genomic diversity of the AQP gene family in pepper. |
| first_indexed | 2024-03-10T11:10:33Z |
| format | Article |
| id | doaj.art-9fd89653596c46ccb29422f27e75b990 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T11:10:33Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-9fd89653596c46ccb29422f27e75b9902023-11-21T20:48:21ZengMDPI AGPlants2223-77472021-05-01106103910.3390/plants10061039Comparative Analysis of Re-Annotated Genes Provides Insight into Evolutionary Divergence and Expressions of Aquaporin Family in PepperYeon Mi Lee0Geun Young Chae1Min Kyung Kim2Seungill Kim3Department of Environmental Horticulture, University of Seoul, Seoul 02504, KoreaDepartment of Environmental Horticulture, University of Seoul, Seoul 02504, KoreaDepartment of Environmental Horticulture, University of Seoul, Seoul 02504, KoreaDepartment of Environmental Horticulture, University of Seoul, Seoul 02504, KoreaAquaporins (AQPs) are known to have a vital role in water transport in all living organisms including agriculturally important crops, but a comprehensive genomic study of AQPs in pepper has not been implemented. Here, we updated previous gene annotations and generated a total of 259 AQP genes from five plants, including pepper. Phylogenetic and motif analyses revealed that a large proportion of pepper AQP genes belong to the specific subgroup of tonoplast intrinsic protein (TIP) subfamily, TIP4. Chromosomal localization and estimated duplication times illustrated that genes in TIP4 formed a tandem array on the short arm of chromosome 1, resulting from pepper-specific expansion after its divergence with <i>Solanaceae</i> species. Transcriptome analyses under various abiotic stress conditions revealed that transport-, photosystem-, and thylakoid-related genes were generally enriched in expression clusters containing AQP genes in pepper. These results provide valuable genomic resources and insight into the evolutionary mechanism that generate genomic diversity of the AQP gene family in pepper.https://www.mdpi.com/2223-7747/10/6/1039aquaporinwater transportre-annotationpepperabiotic stress |
| spellingShingle | Yeon Mi Lee Geun Young Chae Min Kyung Kim Seungill Kim Comparative Analysis of Re-Annotated Genes Provides Insight into Evolutionary Divergence and Expressions of Aquaporin Family in Pepper Plants aquaporin water transport re-annotation pepper abiotic stress |
| title | Comparative Analysis of Re-Annotated Genes Provides Insight into Evolutionary Divergence and Expressions of Aquaporin Family in Pepper |
| title_full | Comparative Analysis of Re-Annotated Genes Provides Insight into Evolutionary Divergence and Expressions of Aquaporin Family in Pepper |
| title_fullStr | Comparative Analysis of Re-Annotated Genes Provides Insight into Evolutionary Divergence and Expressions of Aquaporin Family in Pepper |
| title_full_unstemmed | Comparative Analysis of Re-Annotated Genes Provides Insight into Evolutionary Divergence and Expressions of Aquaporin Family in Pepper |
| title_short | Comparative Analysis of Re-Annotated Genes Provides Insight into Evolutionary Divergence and Expressions of Aquaporin Family in Pepper |
| title_sort | comparative analysis of re annotated genes provides insight into evolutionary divergence and expressions of aquaporin family in pepper |
| topic | aquaporin water transport re-annotation pepper abiotic stress |
| url | https://www.mdpi.com/2223-7747/10/6/1039 |
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