Study of associations of blood proteins with development of unstable atherosclerotic plaques in coronary arteries by quantitative proteomics
Aim. To study the associations of blood proteins with the presence of unstable atherosclerotic plaques in the arteries in patients with coronary artery disease using the quantitative proteomic analysis.Materials and methods. The study included patients with coronary artery disease (n = 40); the aver...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Siberian State Medical University (Tomsk)
2023-01-01
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| Series: | Бюллетень сибирской медицины |
| Subjects: | |
| Online Access: | https://bulletin.ssmu.ru/jour/article/view/5032 |
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| author | E. M. Stakhneva E. V. Kashtanova Ya. V. Polonskaya E. V. Striukova V. S. Shramko E. V. Sadovski A. V. Kurguzov I. S. Murashov A. M. Chernyavskii Yu. I. Ragino |
| author_facet | E. M. Stakhneva E. V. Kashtanova Ya. V. Polonskaya E. V. Striukova V. S. Shramko E. V. Sadovski A. V. Kurguzov I. S. Murashov A. M. Chernyavskii Yu. I. Ragino |
| author_sort | E. M. Stakhneva |
| collection | DOAJ |
| description | Aim. To study the associations of blood proteins with the presence of unstable atherosclerotic plaques in the arteries in patients with coronary artery disease using the quantitative proteomic analysis.Materials and methods. The study included patients with coronary artery disease (n = 40); the average age of patients was 58 ± 7 years. Material for the study was blood serum. Protein concentrations in serum samples were determined using the PeptiQuant Plus Proteomics Kit (Cambridge Isotope Laboratories, USA). Protein fractions were identified using the liquid chromatograph and tandem mass spectrometer Q-TRAP 6500.Results. Mass spectrometry revealed an increased concentration of proteins, such as fibrinogen, fibulin-1, and complement factor H, in the serum samples of patients with unstable atherosclerotic plaques. It took place with a simultaneous decrease in the levels of α 2-antiplasmin, heparin cofactor II, coagulation factor XII, plasminogen, prothrombin, vitronectin, complement proteins (C1, C3, C7, C9), and complement factor B. The differences were considered significant at p < 0.05. It was revealed that the presence of unstable atherosclerotic plaques was associated with the level of fibulin-1 (Exp(B) = 1.008; р = 0.05), plasminogen (Exp(В) = 0.995; р = 0.027), and coagulation factor X (Exp(В) = 0.973; р = 0.037).Conclusion. An increased concentration of fibulin-1 can be considered as a potential biomarker of unstable atherosclerotic plaque development in coronary artery disease. The possibility of using the studied proteins as biomarkers of unstable atherosclerotic plaques requires further studies on their potential role in the development of this disease. |
| first_indexed | 2024-04-10T01:05:31Z |
| format | Article |
| id | doaj.art-9fd8e3cd6fc74e7486ea6aa85653d5c4 |
| institution | Directory Open Access Journal |
| issn | 1682-0363 1819-3684 |
| language | English |
| last_indexed | 2024-04-10T01:05:31Z |
| publishDate | 2023-01-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj.art-9fd8e3cd6fc74e7486ea6aa85653d5c42023-03-13T09:58:28ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842023-01-0121412112910.20538/1682-0363-2022-4-121-1292949Study of associations of blood proteins with development of unstable atherosclerotic plaques in coronary arteries by quantitative proteomicsE. M. Stakhneva0E. V. Kashtanova1Ya. V. Polonskaya2E. V. Striukova3V. S. Shramko4E. V. Sadovski5A. V. Kurguzov6I. S. Murashov7A. M. Chernyavskii8Yu. I. Ragino9Научно-исследовательский институт терапии и профилактической медицины – филиал Федерального исследовательского центра «Институт цитологии и генетики Сибирского отделения Российской академии наук» (НИИТПМ – филиал ИЦиГ СО РАН)Научно-исследовательский институт терапии и профилактической медицины – филиал Федерального исследовательского центра «Институт цитологии и генетики Сибирского отделения Российской академии наук» (НИИТПМ – филиал ИЦиГ СО РАН)Научно-исследовательский институт терапии и профилактической медицины – филиал Федерального исследовательского центра «Институт цитологии и генетики Сибирского отделения Российской академии наук» (НИИТПМ – филиал ИЦиГ СО РАН)Научно-исследовательский институт терапии и профилактической медицины – филиал Федерального исследовательского центра «Институт цитологии и генетики Сибирского отделения Российской академии наук» (НИИТПМ – филиал ИЦиГ СО РАН)Научно-исследовательский институт терапии и профилактической медицины – филиал Федерального исследовательского центра «Институт цитологии и генетики Сибирского отделения Российской академии наук» (НИИТПМ – филиал ИЦиГ СО РАН)Научно-исследовательский институт терапии и профилактической медицины – филиал Федерального исследовательского центра «Институт цитологии и генетики Сибирского отделения Российской академии наук» (НИИТПМ – филиал ИЦиГ СО РАН)Национальный медицинский исследовательский центр (НМИЦ) им. акад. Е.Н. МешалкинаНациональный медицинский исследовательский центр (НМИЦ) им. акад. Е.Н. МешалкинаНациональный медицинский исследовательский центр (НМИЦ) им. акад. Е.Н. МешалкинаНаучно-исследовательский институт терапии и профилактической медицины – филиал Федерального исследовательского центра «Институт цитологии и генетики Сибирского отделения Российской академии наук» (НИИТПМ – филиал ИЦиГ СО РАН)Aim. To study the associations of blood proteins with the presence of unstable atherosclerotic plaques in the arteries in patients with coronary artery disease using the quantitative proteomic analysis.Materials and methods. The study included patients with coronary artery disease (n = 40); the average age of patients was 58 ± 7 years. Material for the study was blood serum. Protein concentrations in serum samples were determined using the PeptiQuant Plus Proteomics Kit (Cambridge Isotope Laboratories, USA). Protein fractions were identified using the liquid chromatograph and tandem mass spectrometer Q-TRAP 6500.Results. Mass spectrometry revealed an increased concentration of proteins, such as fibrinogen, fibulin-1, and complement factor H, in the serum samples of patients with unstable atherosclerotic plaques. It took place with a simultaneous decrease in the levels of α 2-antiplasmin, heparin cofactor II, coagulation factor XII, plasminogen, prothrombin, vitronectin, complement proteins (C1, C3, C7, C9), and complement factor B. The differences were considered significant at p < 0.05. It was revealed that the presence of unstable atherosclerotic plaques was associated with the level of fibulin-1 (Exp(B) = 1.008; р = 0.05), plasminogen (Exp(В) = 0.995; р = 0.027), and coagulation factor X (Exp(В) = 0.973; р = 0.037).Conclusion. An increased concentration of fibulin-1 can be considered as a potential biomarker of unstable atherosclerotic plaque development in coronary artery disease. The possibility of using the studied proteins as biomarkers of unstable atherosclerotic plaques requires further studies on their potential role in the development of this disease.https://bulletin.ssmu.ru/jour/article/view/5032протеомный анализмасс-спектрометриякоронарный атеросклероз |
| spellingShingle | E. M. Stakhneva E. V. Kashtanova Ya. V. Polonskaya E. V. Striukova V. S. Shramko E. V. Sadovski A. V. Kurguzov I. S. Murashov A. M. Chernyavskii Yu. I. Ragino Study of associations of blood proteins with development of unstable atherosclerotic plaques in coronary arteries by quantitative proteomics Бюллетень сибирской медицины протеомный анализ масс-спектрометрия коронарный атеросклероз |
| title | Study of associations of blood proteins with development of unstable atherosclerotic plaques in coronary arteries by quantitative proteomics |
| title_full | Study of associations of blood proteins with development of unstable atherosclerotic plaques in coronary arteries by quantitative proteomics |
| title_fullStr | Study of associations of blood proteins with development of unstable atherosclerotic plaques in coronary arteries by quantitative proteomics |
| title_full_unstemmed | Study of associations of blood proteins with development of unstable atherosclerotic plaques in coronary arteries by quantitative proteomics |
| title_short | Study of associations of blood proteins with development of unstable atherosclerotic plaques in coronary arteries by quantitative proteomics |
| title_sort | study of associations of blood proteins with development of unstable atherosclerotic plaques in coronary arteries by quantitative proteomics |
| topic | протеомный анализ масс-спектрометрия коронарный атеросклероз |
| url | https://bulletin.ssmu.ru/jour/article/view/5032 |
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