The interaction of dendritic cells and γδ T cells promotes the activation of γδ T cells in experimental autoimmune uveitis
Uveitis is a severe inflammatory disease that can cause visual impairment. Recently, activated γδ T cells were proved to play a central role in the development of experimental autoimmune uveitis (EAU). However, the mechanism underlying γδ T-cell activation in EAU is incompletely known. In this study...
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| Format: | Article |
| Language: | English |
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World Scientific Publishing
2017-03-01
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| Series: | Journal of Innovative Optical Health Sciences |
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| Online Access: | http://www.worldscientific.com/doi/pdf/10.1142/S1793545816500425 |
| _version_ | 1819243452976594944 |
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| author | Beibei Wang Wei Lin Jike Song Xiaofeng Xie Hongsheng Bi |
| author_facet | Beibei Wang Wei Lin Jike Song Xiaofeng Xie Hongsheng Bi |
| author_sort | Beibei Wang |
| collection | DOAJ |
| description | Uveitis is a severe inflammatory disease that can cause visual impairment. Recently, activated γδ T cells were proved to play a central role in the development of experimental autoimmune uveitis (EAU). However, the mechanism underlying γδ T-cell activation in EAU is incompletely known. In this study, we determined the percentage changes in and the phenotypes of γδ T cells and dendritic cells (DCs) obtained from the spleens of immunized C57BL/6 (B6) mice, an animal model of EAU. We found that the number of γδ T cells and DCs obviously increased during the inflammation phase of EAU (days 16–20 of our experiment), and that during this time, γδ T cells expressed high levels of CD69 and the integrin lymphocyte function–associated antigen-1 (LFA-1) and secreted high levels of interleukin (IL)-17A. Moreover, DCs obtained during this phase expressed high levels of CD80, CD83, CD86, and intracellular cell adhesion molecule-1 (ICAM-1). Furthermore, we studied the interaction between DCs and γδ T cells by using flow cytometry and confocal microscopy in order to determine whether DCs affected γδ T-cell activation in vitro. Co-cultures of the two types of cells showed that DCs induced high levels of CD69, LFA-1, and IL-17A in γδ T cells. Imaging studies revealed contact between the DCs and γδ T cells. This interaction was mediated by the accumulation of ICAM-1 and LFA-1 at the interface of DCs-γδ T cells. Thus, the activation of γδ T cells in EAU was promoted by DCs interacting with γδ T cells. |
| first_indexed | 2024-12-23T14:55:56Z |
| format | Article |
| id | doaj.art-9fd90ebd5d55462c9be916d8dad7fd9d |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-12-23T14:55:56Z |
| publishDate | 2017-03-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-9fd90ebd5d55462c9be916d8dad7fd9d2022-12-21T17:42:45ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052017-03-011021650042-11650042-1210.1142/S179354581650042510.1142/S1793545816500425The interaction of dendritic cells and γδ T cells promotes the activation of γδ T cells in experimental autoimmune uveitisBeibei Wang0Wei Lin1Jike Song2Xiaofeng Xie3Hongsheng Bi4Shandong University of Traditional Chinese Medicine, 4655#, Daxue Road, Jinan City 250355, P. R. ChinaDepartment of Microbiology, Shandong Academy of Medical Sciences, Jinan City 250030, P. R. ChinaShandong University of Traditional Chinese Medicine, 4655#, Daxue Road, Jinan City 250355, P. R. ChinaAffiliated Eye Hospital, Shandong University of Traditional Chinese Medicine, 48#, Yingxiongshan Road, Jinan City 250002, P. R. ChinaEye Institute of Shandong University of Traditional Chinese Medicine, 48#, Yingxiongshan Road, Jinan City 250002, P. R. ChinaUveitis is a severe inflammatory disease that can cause visual impairment. Recently, activated γδ T cells were proved to play a central role in the development of experimental autoimmune uveitis (EAU). However, the mechanism underlying γδ T-cell activation in EAU is incompletely known. In this study, we determined the percentage changes in and the phenotypes of γδ T cells and dendritic cells (DCs) obtained from the spleens of immunized C57BL/6 (B6) mice, an animal model of EAU. We found that the number of γδ T cells and DCs obviously increased during the inflammation phase of EAU (days 16–20 of our experiment), and that during this time, γδ T cells expressed high levels of CD69 and the integrin lymphocyte function–associated antigen-1 (LFA-1) and secreted high levels of interleukin (IL)-17A. Moreover, DCs obtained during this phase expressed high levels of CD80, CD83, CD86, and intracellular cell adhesion molecule-1 (ICAM-1). Furthermore, we studied the interaction between DCs and γδ T cells by using flow cytometry and confocal microscopy in order to determine whether DCs affected γδ T-cell activation in vitro. Co-cultures of the two types of cells showed that DCs induced high levels of CD69, LFA-1, and IL-17A in γδ T cells. Imaging studies revealed contact between the DCs and γδ T cells. This interaction was mediated by the accumulation of ICAM-1 and LFA-1 at the interface of DCs-γδ T cells. Thus, the activation of γδ T cells in EAU was promoted by DCs interacting with γδ T cells.http://www.worldscientific.com/doi/pdf/10.1142/S1793545816500425γδ T cellsdendritic cellsexperimental autoimmune uveitisintegrin lymphocyte function–associated antigen-1 (LFA-1) |
| spellingShingle | Beibei Wang Wei Lin Jike Song Xiaofeng Xie Hongsheng Bi The interaction of dendritic cells and γδ T cells promotes the activation of γδ T cells in experimental autoimmune uveitis Journal of Innovative Optical Health Sciences γδ T cells dendritic cells experimental autoimmune uveitis integrin lymphocyte function–associated antigen-1 (LFA-1) |
| title | The interaction of dendritic cells and γδ T cells promotes the activation of γδ T cells in experimental autoimmune uveitis |
| title_full | The interaction of dendritic cells and γδ T cells promotes the activation of γδ T cells in experimental autoimmune uveitis |
| title_fullStr | The interaction of dendritic cells and γδ T cells promotes the activation of γδ T cells in experimental autoimmune uveitis |
| title_full_unstemmed | The interaction of dendritic cells and γδ T cells promotes the activation of γδ T cells in experimental autoimmune uveitis |
| title_short | The interaction of dendritic cells and γδ T cells promotes the activation of γδ T cells in experimental autoimmune uveitis |
| title_sort | interaction of dendritic cells and γδ t cells promotes the activation of γδ t cells in experimental autoimmune uveitis |
| topic | γδ T cells dendritic cells experimental autoimmune uveitis integrin lymphocyte function–associated antigen-1 (LFA-1) |
| url | http://www.worldscientific.com/doi/pdf/10.1142/S1793545816500425 |
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