Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteria
Background: Marine ecosystems contain benthic microalgae and bacterial species that are capable of secreting extracellular polymeric substances (EPS), suggesting that settlement of these microorganisms can occur on submerged surfaces, a key part of the first stage of biofouling. Currently, anti-foul...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-05-01
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| Series: | Electronic Journal of Biotechnology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345818300071 |
| _version_ | 1818292725472034816 |
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| author | Claudia D. Infante Francisca Castillo Vilma Pérez Carlos Riquelme |
| author_facet | Claudia D. Infante Francisca Castillo Vilma Pérez Carlos Riquelme |
| author_sort | Claudia D. Infante |
| collection | DOAJ |
| description | Background: Marine ecosystems contain benthic microalgae and bacterial species that are capable of secreting extracellular polymeric substances (EPS), suggesting that settlement of these microorganisms can occur on submerged surfaces, a key part of the first stage of biofouling. Currently, anti-fouling treatments that help control this phenomenon involve the use of biocides or antifouling paints that contain heavy metals, which over a long period of exposure can spread to the environment. The bacterium Alteromonas sp. Ni1-LEM has an inhibitory effect on the adhesion of Nitzschia ovalis, an abundant diatom found on submerged surfaces. Results: We evaluated the effect of the bioactive compound secreted by this bacterium on the EPS of biofilms and associated epiphytic bacteria. Three methods of EPS extraction were evaluated to determine the most appropriate and efficient methodology based on the presence of soluble EPS and the total protein and carbohydrate concentrations. Microalgae were cultured with the bacterial compound to evaluate its effect on EPS secretion and variations in its protein and carbohydrate concentrations. An effect of the bacterial supernatant on EPS was observed by assessing biofilm formation and changes in the concentration of proteins and carbohydrates present in the biofilm. Conclusions: These results indicate that a possible mechanism for regulating biofouling could be through alteration of biofilm EPS and alteration of the epiphytic bacterial community associated with the microalga.How to cite: Infante, C.D., Castillo, F., Pérez, V., et al. Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteria. Electron J Biotechnol 2018;33 https://doi.org/10.1016/j.ejbt.2018.03.002. Keywords: Anti-fouling, Benthic microalgae, Biofilm, Biofouling, Epiphytic bacterial community, EPS, Marine ecosystems, Metagenomic, Nitzschia ovalis, Settlement inhibition, Submerged surfaces |
| first_indexed | 2024-12-13T03:04:31Z |
| format | Article |
| id | doaj.art-9fdad2598cd94058be1e983d26683a7e |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-13T03:04:31Z |
| publishDate | 2018-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-9fdad2598cd94058be1e983d26683a7e2022-12-22T00:01:45ZengElsevierElectronic Journal of Biotechnology0717-34582018-05-0133110Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteriaClaudia D. Infante0Francisca Castillo1Vilma Pérez2Carlos Riquelme3Laboratorio de Ecología Microbiana, Centro de Bioinnovación, Facultad de Ciencias del Mar y Recursos Biológicos, Universidad de Antofagasta, Antofagasta, Chile; Corresponding author.Laboratorio de Ecología Microbiana, Centro de Bioinnovación, Facultad de Ciencias del Mar y Recursos Biológicos, Universidad de Antofagasta, Antofagasta, ChileLaboratorio de Ecología Molecular y Microbiología Aplicada, Departamento de Ciencias Farmacéuticas, Facultad de Ciencias, Universidad Católica del Norte, Antofagasta, ChileLaboratorio de Ecología Microbiana, Centro de Bioinnovación, Facultad de Ciencias del Mar y Recursos Biológicos, Universidad de Antofagasta, Antofagasta, ChileBackground: Marine ecosystems contain benthic microalgae and bacterial species that are capable of secreting extracellular polymeric substances (EPS), suggesting that settlement of these microorganisms can occur on submerged surfaces, a key part of the first stage of biofouling. Currently, anti-fouling treatments that help control this phenomenon involve the use of biocides or antifouling paints that contain heavy metals, which over a long period of exposure can spread to the environment. The bacterium Alteromonas sp. Ni1-LEM has an inhibitory effect on the adhesion of Nitzschia ovalis, an abundant diatom found on submerged surfaces. Results: We evaluated the effect of the bioactive compound secreted by this bacterium on the EPS of biofilms and associated epiphytic bacteria. Three methods of EPS extraction were evaluated to determine the most appropriate and efficient methodology based on the presence of soluble EPS and the total protein and carbohydrate concentrations. Microalgae were cultured with the bacterial compound to evaluate its effect on EPS secretion and variations in its protein and carbohydrate concentrations. An effect of the bacterial supernatant on EPS was observed by assessing biofilm formation and changes in the concentration of proteins and carbohydrates present in the biofilm. Conclusions: These results indicate that a possible mechanism for regulating biofouling could be through alteration of biofilm EPS and alteration of the epiphytic bacterial community associated with the microalga.How to cite: Infante, C.D., Castillo, F., Pérez, V., et al. Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteria. Electron J Biotechnol 2018;33 https://doi.org/10.1016/j.ejbt.2018.03.002. Keywords: Anti-fouling, Benthic microalgae, Biofilm, Biofouling, Epiphytic bacterial community, EPS, Marine ecosystems, Metagenomic, Nitzschia ovalis, Settlement inhibition, Submerged surfaceshttp://www.sciencedirect.com/science/article/pii/S0717345818300071 |
| spellingShingle | Claudia D. Infante Francisca Castillo Vilma Pérez Carlos Riquelme Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteria Electronic Journal of Biotechnology |
| title | Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteria |
| title_full | Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteria |
| title_fullStr | Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteria |
| title_full_unstemmed | Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteria |
| title_short | Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteria |
| title_sort | inhibition of nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of eps and epiphytic bacteria |
| url | http://www.sciencedirect.com/science/article/pii/S0717345818300071 |
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