Evaluation of the expression of bone marrow‐derived mesenchymal stem cells and cancer‐associated fibroblasts in the stroma of gastric cancer tissue
Abstract Aim Cancer‐associated fibroblasts (CAFs) generated by bone marrow‐derived mesenchymal stem cells (BM‐MSCs) play an important role in cancer progression. In this study, we investigated the relationships of BM‐MSCs and CAFs in resected gastric cancers with the clinicopathological factors of p...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-07-01
|
| Series: | Annals of Gastroenterological Surgery |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ags3.12347 |
| _version_ | 1818848833152483328 |
|---|---|
| author | Taizan Minami Keishiro Aoyagi Akihiko Kawahara Naotaka Murakami Taro Isobe Yuya Tanaka Hideaki Kaku Fumihiko Fujita Yoshito Akagi |
| author_facet | Taizan Minami Keishiro Aoyagi Akihiko Kawahara Naotaka Murakami Taro Isobe Yuya Tanaka Hideaki Kaku Fumihiko Fujita Yoshito Akagi |
| author_sort | Taizan Minami |
| collection | DOAJ |
| description | Abstract Aim Cancer‐associated fibroblasts (CAFs) generated by bone marrow‐derived mesenchymal stem cells (BM‐MSCs) play an important role in cancer progression. In this study, we investigated the relationships of BM‐MSCs and CAFs in resected gastric cancers with the clinicopathological factors of patients. Methods We analyzed 120 gastric cancer patients who underwent gastrectomy. Immunostaining was performed with an anti‐CD271 antibody (BM‐MSCs) and anti‐α‐smooth muscle actin (αSMA) antibody (CAFs). Staining intensity was used to divide patients into low and high expression groups. Observation sites in cancer tissues were invasive, central, and whole portions. Results Expression of αSMA was significantly related to depth of tumor invasion (T), lymph node metastasis (N), lymphatic invasion (ly), venous invasion (v), and stage. Expression of CD271 was significantly related to v, stage, stromal volume, and tumor infiltration pattern (INF). Overall survival (OS) of the high expression group was significantly lower than that of the low expression group for both αSMA and CD271. Multivariate analysis showed that N, αSMA (whole), and CD271 (invasive) were independent prognostic factors. Conclusions Cancer‐associated fibroblasts and BM‐MSCs are related to the progression, invasion, and prognosis of gastric cancer and may be therapeutic targets of gastric cancer. |
| first_indexed | 2024-12-19T06:23:37Z |
| format | Article |
| id | doaj.art-9fdb5f0c22f8434480b6e01169901680 |
| institution | Directory Open Access Journal |
| issn | 2475-0328 |
| language | English |
| last_indexed | 2024-12-19T06:23:37Z |
| publishDate | 2020-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Annals of Gastroenterological Surgery |
| spelling | doaj.art-9fdb5f0c22f8434480b6e011699016802022-12-21T20:32:38ZengWileyAnnals of Gastroenterological Surgery2475-03282020-07-014446447410.1002/ags3.12347Evaluation of the expression of bone marrow‐derived mesenchymal stem cells and cancer‐associated fibroblasts in the stroma of gastric cancer tissueTaizan Minami0Keishiro Aoyagi1Akihiko Kawahara2Naotaka Murakami3Taro Isobe4Yuya Tanaka5Hideaki Kaku6Fumihiko Fujita7Yoshito Akagi8Department of Surgery Kurume University School of Medicine Fukuoka JapanDepartment of Surgery Kurume University School of Medicine Fukuoka JapanDepartment of Diagnostic Pathology Kurume University School of Medicine Fukuoka JapanDepartment of Surgery Kurume University School of Medicine Fukuoka JapanDepartment of Surgery Kurume University School of Medicine Fukuoka JapanDepartment of Surgery Kurume University School of Medicine Fukuoka JapanDepartment of Surgery Kurume University School of Medicine Fukuoka JapanDepartment of Surgery Kurume University School of Medicine Fukuoka JapanDepartment of Surgery Kurume University School of Medicine Fukuoka JapanAbstract Aim Cancer‐associated fibroblasts (CAFs) generated by bone marrow‐derived mesenchymal stem cells (BM‐MSCs) play an important role in cancer progression. In this study, we investigated the relationships of BM‐MSCs and CAFs in resected gastric cancers with the clinicopathological factors of patients. Methods We analyzed 120 gastric cancer patients who underwent gastrectomy. Immunostaining was performed with an anti‐CD271 antibody (BM‐MSCs) and anti‐α‐smooth muscle actin (αSMA) antibody (CAFs). Staining intensity was used to divide patients into low and high expression groups. Observation sites in cancer tissues were invasive, central, and whole portions. Results Expression of αSMA was significantly related to depth of tumor invasion (T), lymph node metastasis (N), lymphatic invasion (ly), venous invasion (v), and stage. Expression of CD271 was significantly related to v, stage, stromal volume, and tumor infiltration pattern (INF). Overall survival (OS) of the high expression group was significantly lower than that of the low expression group for both αSMA and CD271. Multivariate analysis showed that N, αSMA (whole), and CD271 (invasive) were independent prognostic factors. Conclusions Cancer‐associated fibroblasts and BM‐MSCs are related to the progression, invasion, and prognosis of gastric cancer and may be therapeutic targets of gastric cancer.https://doi.org/10.1002/ags3.12347BM‐MSCCAFgastric cancerimmunohistochemistry |
| spellingShingle | Taizan Minami Keishiro Aoyagi Akihiko Kawahara Naotaka Murakami Taro Isobe Yuya Tanaka Hideaki Kaku Fumihiko Fujita Yoshito Akagi Evaluation of the expression of bone marrow‐derived mesenchymal stem cells and cancer‐associated fibroblasts in the stroma of gastric cancer tissue Annals of Gastroenterological Surgery BM‐MSC CAF gastric cancer immunohistochemistry |
| title | Evaluation of the expression of bone marrow‐derived mesenchymal stem cells and cancer‐associated fibroblasts in the stroma of gastric cancer tissue |
| title_full | Evaluation of the expression of bone marrow‐derived mesenchymal stem cells and cancer‐associated fibroblasts in the stroma of gastric cancer tissue |
| title_fullStr | Evaluation of the expression of bone marrow‐derived mesenchymal stem cells and cancer‐associated fibroblasts in the stroma of gastric cancer tissue |
| title_full_unstemmed | Evaluation of the expression of bone marrow‐derived mesenchymal stem cells and cancer‐associated fibroblasts in the stroma of gastric cancer tissue |
| title_short | Evaluation of the expression of bone marrow‐derived mesenchymal stem cells and cancer‐associated fibroblasts in the stroma of gastric cancer tissue |
| title_sort | evaluation of the expression of bone marrow derived mesenchymal stem cells and cancer associated fibroblasts in the stroma of gastric cancer tissue |
| topic | BM‐MSC CAF gastric cancer immunohistochemistry |
| url | https://doi.org/10.1002/ags3.12347 |
| work_keys_str_mv | AT taizanminami evaluationoftheexpressionofbonemarrowderivedmesenchymalstemcellsandcancerassociatedfibroblastsinthestromaofgastriccancertissue AT keishiroaoyagi evaluationoftheexpressionofbonemarrowderivedmesenchymalstemcellsandcancerassociatedfibroblastsinthestromaofgastriccancertissue AT akihikokawahara evaluationoftheexpressionofbonemarrowderivedmesenchymalstemcellsandcancerassociatedfibroblastsinthestromaofgastriccancertissue AT naotakamurakami evaluationoftheexpressionofbonemarrowderivedmesenchymalstemcellsandcancerassociatedfibroblastsinthestromaofgastriccancertissue AT taroisobe evaluationoftheexpressionofbonemarrowderivedmesenchymalstemcellsandcancerassociatedfibroblastsinthestromaofgastriccancertissue AT yuyatanaka evaluationoftheexpressionofbonemarrowderivedmesenchymalstemcellsandcancerassociatedfibroblastsinthestromaofgastriccancertissue AT hideakikaku evaluationoftheexpressionofbonemarrowderivedmesenchymalstemcellsandcancerassociatedfibroblastsinthestromaofgastriccancertissue AT fumihikofujita evaluationoftheexpressionofbonemarrowderivedmesenchymalstemcellsandcancerassociatedfibroblastsinthestromaofgastriccancertissue AT yoshitoakagi evaluationoftheexpressionofbonemarrowderivedmesenchymalstemcellsandcancerassociatedfibroblastsinthestromaofgastriccancertissue |