Revisiting the Hybridization Processes in the <i>Triatoma brasiliensis</i> Complex (Hemiptera, Triatominae): Reproductive Isolation between <i>Triatoma petrocchiae</i> and <i>T. b. brasiliensis</i> and <i>T. lenti</i>
<i>Triatoma petrocchiae</i> is a species morphologically similar to <i>T. b. brasiliensis</i> (which resulted in a synonymization event); despite this similarity, genetic, morphological, and experimental crossbreeding studies confirmed the specific status of <i>T. petro...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/11/1015 |
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| author | Luiza Maria Grzyb Delgado Jader de Oliveira Amanda Ravazi Fernanda Fernandez Madeira Yago Visinho dos Reis Heloisa Pinotti Ana Beatriz Bortolozo de Oliveira Isabella da Silva Masarin Maurício Lilioso Elaine Folly-Ramos Cleber Galvão Maria Tercília Vilela de Azeredo-Oliveira João Aristeu da Rosa Kaio Cesar Chaboli Alevi |
| author_facet | Luiza Maria Grzyb Delgado Jader de Oliveira Amanda Ravazi Fernanda Fernandez Madeira Yago Visinho dos Reis Heloisa Pinotti Ana Beatriz Bortolozo de Oliveira Isabella da Silva Masarin Maurício Lilioso Elaine Folly-Ramos Cleber Galvão Maria Tercília Vilela de Azeredo-Oliveira João Aristeu da Rosa Kaio Cesar Chaboli Alevi |
| author_sort | Luiza Maria Grzyb Delgado |
| collection | DOAJ |
| description | <i>Triatoma petrocchiae</i> is a species morphologically similar to <i>T. b. brasiliensis</i> (which resulted in a synonymization event); despite this similarity, genetic, morphological, and experimental crossbreeding studies confirmed the specific status of <i>T. petrocchiae</i>. Considering that both species have been reported living in sympatry and that, for a long time, most species of the <i>T. brasiliensis</i> complex were considered only chromatic variants of <i>T. b. brasiliensis</i>, we carried out experimental crosses between <i>T. b. brasiliensis</i> and <i>T. petrocchiae</i> (to confirm whether these species are reproductively isolated) and between <i>T. lenti</i> and <i>T. petrocchiae</i> (to assess whether <i>T. petrocchiae</i> also presents prezygotic isolation with the other species of the <i>T. brasiliensis</i> complex). Reciprocal experimental crosses were conducted, and weekly, the eggs were collected, counted, and separated in new containers to assess the hatch rate. Neither cross resulted in hybrids, demonstrating that there are pre-zygotic reproductive barriers installed between <i>T. petrocchiae</i> and the other species of the <i>T. brasiliensis</i> complex. On the basis of the results above, we demonstrated that <i>T. petrocchiae</i> is reproductively isolated from <i>T. b. brasiliensis</i> and <i>T. lenti</i>. Furthermore, we suggest that <i>T. petrocchiae</i> is the species most derived from the <i>T. brasiliensis</i> complex. |
| first_indexed | 2024-03-10T05:24:56Z |
| format | Article |
| id | doaj.art-9fdc4e599c354c0f9935a8589795dd70 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T05:24:56Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-9fdc4e599c354c0f9935a8589795dd702023-11-22T23:47:12ZengMDPI AGInsects2075-44502021-11-011211101510.3390/insects12111015Revisiting the Hybridization Processes in the <i>Triatoma brasiliensis</i> Complex (Hemiptera, Triatominae): Reproductive Isolation between <i>Triatoma petrocchiae</i> and <i>T. b. brasiliensis</i> and <i>T. lenti</i>Luiza Maria Grzyb Delgado0Jader de Oliveira1Amanda Ravazi2Fernanda Fernandez Madeira3Yago Visinho dos Reis4Heloisa Pinotti5Ana Beatriz Bortolozo de Oliveira6Isabella da Silva Masarin7Maurício Lilioso8Elaine Folly-Ramos9Cleber Galvão10Maria Tercília Vilela de Azeredo-Oliveira11João Aristeu da Rosa12Kaio Cesar Chaboli Alevi13Instituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Rua Dr. Antônio Celso Wagner Zanin, 250, Distrito de Rubião Júnior, Botucatu 18618-689, BrazilLaboratório de Entomologia em Saúde Pública, Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo (USP), Av. Dr. Arnaldo 715, São Paulo 01246-904, BrazilInstituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Rua Dr. Antônio Celso Wagner Zanin, 250, Distrito de Rubião Júnior, Botucatu 18618-689, BrazilLaboratório de Biologia Celular, Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Câmpus de São José do Rio Preto, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Rua Cristóvão Colombo 2265, São José do Rio Preto 15054-000, BrazilInstituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Rua Dr. Antônio Celso Wagner Zanin, 250, Distrito de Rubião Júnior, Botucatu 18618-689, BrazilLaboratório de Parasitologia, Departamento de Ciências Biológicas, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Faculdade de Ciências Farmacêuticas, Câmpus de Araraquara, Rod. Araraquara-Jaú km 1, Araraquara 14801-902, BrazilLaboratório de Biologia Celular, Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Câmpus de São José do Rio Preto, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Rua Cristóvão Colombo 2265, São José do Rio Preto 15054-000, BrazilInstituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Rua Dr. Antônio Celso Wagner Zanin, 250, Distrito de Rubião Júnior, Botucatu 18618-689, BrazilInstituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Rua Monteiro Lobato, 255, Campinas 13083-862, BrazilCentro de Ciências Aplicadas e Educação, Laboratório de Ecologia Animal, Departamento de Engenharia e Meio Ambiente, Universidade Federal de Paraíba (UFPB), Av. Santa Elizabete, 160, Rio Tinto, Paraíba 58297-000, BrazilLaboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Instituto Oswaldo Cruz (IOC/FIOCRUZ), Av. Brasil 4365, Pavilhão Rocha Lima, Sala 505, Rio de Janeiro 21040-360, BrazilLaboratório de Biologia Celular, Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Câmpus de São José do Rio Preto, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Rua Cristóvão Colombo 2265, São José do Rio Preto 15054-000, BrazilLaboratório de Parasitologia, Departamento de Ciências Biológicas, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Faculdade de Ciências Farmacêuticas, Câmpus de Araraquara, Rod. Araraquara-Jaú km 1, Araraquara 14801-902, BrazilInstituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Rua Dr. Antônio Celso Wagner Zanin, 250, Distrito de Rubião Júnior, Botucatu 18618-689, Brazil<i>Triatoma petrocchiae</i> is a species morphologically similar to <i>T. b. brasiliensis</i> (which resulted in a synonymization event); despite this similarity, genetic, morphological, and experimental crossbreeding studies confirmed the specific status of <i>T. petrocchiae</i>. Considering that both species have been reported living in sympatry and that, for a long time, most species of the <i>T. brasiliensis</i> complex were considered only chromatic variants of <i>T. b. brasiliensis</i>, we carried out experimental crosses between <i>T. b. brasiliensis</i> and <i>T. petrocchiae</i> (to confirm whether these species are reproductively isolated) and between <i>T. lenti</i> and <i>T. petrocchiae</i> (to assess whether <i>T. petrocchiae</i> also presents prezygotic isolation with the other species of the <i>T. brasiliensis</i> complex). Reciprocal experimental crosses were conducted, and weekly, the eggs were collected, counted, and separated in new containers to assess the hatch rate. Neither cross resulted in hybrids, demonstrating that there are pre-zygotic reproductive barriers installed between <i>T. petrocchiae</i> and the other species of the <i>T. brasiliensis</i> complex. On the basis of the results above, we demonstrated that <i>T. petrocchiae</i> is reproductively isolated from <i>T. b. brasiliensis</i> and <i>T. lenti</i>. Furthermore, we suggest that <i>T. petrocchiae</i> is the species most derived from the <i>T. brasiliensis</i> complex.https://www.mdpi.com/2075-4450/12/11/1015triatominesreproductive barriershybridizationspeciationChagas disease vectors |
| spellingShingle | Luiza Maria Grzyb Delgado Jader de Oliveira Amanda Ravazi Fernanda Fernandez Madeira Yago Visinho dos Reis Heloisa Pinotti Ana Beatriz Bortolozo de Oliveira Isabella da Silva Masarin Maurício Lilioso Elaine Folly-Ramos Cleber Galvão Maria Tercília Vilela de Azeredo-Oliveira João Aristeu da Rosa Kaio Cesar Chaboli Alevi Revisiting the Hybridization Processes in the <i>Triatoma brasiliensis</i> Complex (Hemiptera, Triatominae): Reproductive Isolation between <i>Triatoma petrocchiae</i> and <i>T. b. brasiliensis</i> and <i>T. lenti</i> Insects triatomines reproductive barriers hybridization speciation Chagas disease vectors |
| title | Revisiting the Hybridization Processes in the <i>Triatoma brasiliensis</i> Complex (Hemiptera, Triatominae): Reproductive Isolation between <i>Triatoma petrocchiae</i> and <i>T. b. brasiliensis</i> and <i>T. lenti</i> |
| title_full | Revisiting the Hybridization Processes in the <i>Triatoma brasiliensis</i> Complex (Hemiptera, Triatominae): Reproductive Isolation between <i>Triatoma petrocchiae</i> and <i>T. b. brasiliensis</i> and <i>T. lenti</i> |
| title_fullStr | Revisiting the Hybridization Processes in the <i>Triatoma brasiliensis</i> Complex (Hemiptera, Triatominae): Reproductive Isolation between <i>Triatoma petrocchiae</i> and <i>T. b. brasiliensis</i> and <i>T. lenti</i> |
| title_full_unstemmed | Revisiting the Hybridization Processes in the <i>Triatoma brasiliensis</i> Complex (Hemiptera, Triatominae): Reproductive Isolation between <i>Triatoma petrocchiae</i> and <i>T. b. brasiliensis</i> and <i>T. lenti</i> |
| title_short | Revisiting the Hybridization Processes in the <i>Triatoma brasiliensis</i> Complex (Hemiptera, Triatominae): Reproductive Isolation between <i>Triatoma petrocchiae</i> and <i>T. b. brasiliensis</i> and <i>T. lenti</i> |
| title_sort | revisiting the hybridization processes in the i triatoma brasiliensis i complex hemiptera triatominae reproductive isolation between i triatoma petrocchiae i and i t b brasiliensis i and i t lenti i |
| topic | triatomines reproductive barriers hybridization speciation Chagas disease vectors |
| url | https://www.mdpi.com/2075-4450/12/11/1015 |
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