| Summary: | Invertase from Saccharomyces cerevisiae was
immobilized on agarose beads, activated with various
groups (glyoxyl, MANAE or glutaraldehyde), and
on some commercial epoxy supports (Eupergit and
Sepabeads). Very active and stable invertase derivatives
were produced by the adsorption of the enzyme on
MANAE-agarose, MANAE-agarose treated with
glutaraldhyde and glutaraldehyde-agarose supports.
At pH 5.0, these derivatives retained full activity after
24h at 40 ºC and 50 ºC. When assayed at 40 °C and 50
°C, with the pH adjusted to 7.0, the invertase-MANAEagarose
derivative treated with glutaraldehyde retained
80% of the initial activity. Recovered activities of the
derivatives produced with MANAE, MANAE treated
with glutaraldehyde and glutaraldehyde alone were
73.5%, 44.4% and 36.8%, respectively. These three
preparations were successfully employed to produce
glucose and fructose in 3 cycles of sucrose hydrolysis.
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