Hen Egg White Lysozyme (HEWL) Confers Resistance to Verticillium Wilt in Cotton by Inhibiting the Spread of Fungus and Generating ROS Burst
Verticillium wilt is a soil-borne vascular disease caused by the fungal pathogen <i>Verticillium dahliae</i>. It causes great harm to upland cotton (<i>Gossypium hirsutum</i>) yield and quality. A previous study has shown that Hen egg white lysozyme (HEWL) exerts strong inhib...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/24/17164 |
| _version_ | 1797380781082935296 |
|---|---|
| author | Wenfang Guo Gangqiang Li Nan Wang Caifeng Yang Huakang Peng Mengqi Wang Dehu Liu |
| author_facet | Wenfang Guo Gangqiang Li Nan Wang Caifeng Yang Huakang Peng Mengqi Wang Dehu Liu |
| author_sort | Wenfang Guo |
| collection | DOAJ |
| description | Verticillium wilt is a soil-borne vascular disease caused by the fungal pathogen <i>Verticillium dahliae</i>. It causes great harm to upland cotton (<i>Gossypium hirsutum</i>) yield and quality. A previous study has shown that Hen egg white lysozyme (HEWL) exerts strong inhibitory activity against <i>V. dahliae</i> in vitro. In the current study, we introduced the <i>HEWL</i> gene into cotton through the <i>Agrobacterium</i>-mediated transformation, and the exogenous HEWL protein was successfully expressed in cotton. Our study revealed that HEWL was able to significantly inhibit the proliferation of <i>V. dahlia</i> in cotton. Consequently, the overexpression of HEWL effectively improved the resistance to Verticillium wilt in transgenic cotton. In addition, ROS accumulation and NO content increased rapidly after the <i>V. dahliae</i> inoculation of plant leaves overexpressing HEWL. In addition, the expression of the PR genes was significantly up-regulated. Taken together, our results suggest that HEWL significantly improves resistance to Verticillium wilt by inhibiting the growth of pathogenic fungus, triggering ROS burst, and activating PR genes expression in cotton. |
| first_indexed | 2024-03-08T20:42:00Z |
| format | Article |
| id | doaj.art-9fddebdd1efd4dde93b7a856d20adcdb |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T20:42:00Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9fddebdd1efd4dde93b7a856d20adcdb2023-12-22T14:13:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124241716410.3390/ijms242417164Hen Egg White Lysozyme (HEWL) Confers Resistance to Verticillium Wilt in Cotton by Inhibiting the Spread of Fungus and Generating ROS BurstWenfang Guo0Gangqiang Li1Nan Wang2Caifeng Yang3Huakang Peng4Mengqi Wang5Dehu Liu6Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaVerticillium wilt is a soil-borne vascular disease caused by the fungal pathogen <i>Verticillium dahliae</i>. It causes great harm to upland cotton (<i>Gossypium hirsutum</i>) yield and quality. A previous study has shown that Hen egg white lysozyme (HEWL) exerts strong inhibitory activity against <i>V. dahliae</i> in vitro. In the current study, we introduced the <i>HEWL</i> gene into cotton through the <i>Agrobacterium</i>-mediated transformation, and the exogenous HEWL protein was successfully expressed in cotton. Our study revealed that HEWL was able to significantly inhibit the proliferation of <i>V. dahlia</i> in cotton. Consequently, the overexpression of HEWL effectively improved the resistance to Verticillium wilt in transgenic cotton. In addition, ROS accumulation and NO content increased rapidly after the <i>V. dahliae</i> inoculation of plant leaves overexpressing HEWL. In addition, the expression of the PR genes was significantly up-regulated. Taken together, our results suggest that HEWL significantly improves resistance to Verticillium wilt by inhibiting the growth of pathogenic fungus, triggering ROS burst, and activating PR genes expression in cotton.https://www.mdpi.com/1422-0067/24/24/17164Hen egg white lysozymeantifungal proteintransgenic cottondisease resistanceVerticillium wiltROS burst |
| spellingShingle | Wenfang Guo Gangqiang Li Nan Wang Caifeng Yang Huakang Peng Mengqi Wang Dehu Liu Hen Egg White Lysozyme (HEWL) Confers Resistance to Verticillium Wilt in Cotton by Inhibiting the Spread of Fungus and Generating ROS Burst International Journal of Molecular Sciences Hen egg white lysozyme antifungal protein transgenic cotton disease resistance Verticillium wilt ROS burst |
| title | Hen Egg White Lysozyme (HEWL) Confers Resistance to Verticillium Wilt in Cotton by Inhibiting the Spread of Fungus and Generating ROS Burst |
| title_full | Hen Egg White Lysozyme (HEWL) Confers Resistance to Verticillium Wilt in Cotton by Inhibiting the Spread of Fungus and Generating ROS Burst |
| title_fullStr | Hen Egg White Lysozyme (HEWL) Confers Resistance to Verticillium Wilt in Cotton by Inhibiting the Spread of Fungus and Generating ROS Burst |
| title_full_unstemmed | Hen Egg White Lysozyme (HEWL) Confers Resistance to Verticillium Wilt in Cotton by Inhibiting the Spread of Fungus and Generating ROS Burst |
| title_short | Hen Egg White Lysozyme (HEWL) Confers Resistance to Verticillium Wilt in Cotton by Inhibiting the Spread of Fungus and Generating ROS Burst |
| title_sort | hen egg white lysozyme hewl confers resistance to verticillium wilt in cotton by inhibiting the spread of fungus and generating ros burst |
| topic | Hen egg white lysozyme antifungal protein transgenic cotton disease resistance Verticillium wilt ROS burst |
| url | https://www.mdpi.com/1422-0067/24/24/17164 |
| work_keys_str_mv | AT wenfangguo heneggwhitelysozymehewlconfersresistancetoverticilliumwiltincottonbyinhibitingthespreadoffungusandgeneratingrosburst AT gangqiangli heneggwhitelysozymehewlconfersresistancetoverticilliumwiltincottonbyinhibitingthespreadoffungusandgeneratingrosburst AT nanwang heneggwhitelysozymehewlconfersresistancetoverticilliumwiltincottonbyinhibitingthespreadoffungusandgeneratingrosburst AT caifengyang heneggwhitelysozymehewlconfersresistancetoverticilliumwiltincottonbyinhibitingthespreadoffungusandgeneratingrosburst AT huakangpeng heneggwhitelysozymehewlconfersresistancetoverticilliumwiltincottonbyinhibitingthespreadoffungusandgeneratingrosburst AT mengqiwang heneggwhitelysozymehewlconfersresistancetoverticilliumwiltincottonbyinhibitingthespreadoffungusandgeneratingrosburst AT dehuliu heneggwhitelysozymehewlconfersresistancetoverticilliumwiltincottonbyinhibitingthespreadoffungusandgeneratingrosburst |