Periostin overexpression in scleroderma cardiac tissue and its utility as a marker for disease complications
Abstract Objective To evaluate the levels of periostin in patients with systemic sclerosis (SSc) and their association with features of systemic sclerosis. Methods The levels of periostin were assessed in the serum of 106 SSc patients and 22 healthy controls and by immunofluorescence staining in car...
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | Arthritis Research & Therapy |
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| Online Access: | https://doi.org/10.1186/s13075-022-02943-2 |
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| author | Fatima El-Adili Justin K. Lui Mortada Najem Giuseppina Farina Maria Trojanowska Flora Sam Andreea M. Bujor |
| author_facet | Fatima El-Adili Justin K. Lui Mortada Najem Giuseppina Farina Maria Trojanowska Flora Sam Andreea M. Bujor |
| author_sort | Fatima El-Adili |
| collection | DOAJ |
| description | Abstract Objective To evaluate the levels of periostin in patients with systemic sclerosis (SSc) and their association with features of systemic sclerosis. Methods The levels of periostin were assessed in the serum of 106 SSc patients and 22 healthy controls and by immunofluorescence staining in cardiac tissue from 4 SSc patients and 4 controls. Serum periostin was measured via enzyme-linked immunosorbent assay. The results were analyzed using Mann-Whitney test or Kruskal-Wallis test followed by Dunn’s multiple comparisons tests and Spearman’s test for correlations. Cardiac tissue from SSc patients and controls was stained for periostin and co-stained for periostin and collagen type I using immunofluorescence. Results Periostin levels were higher in patients with SSc compared to controls and directly correlated to modified Rodnan skin score and echocardiography parameters of left ventricular measurements. Immunofluorescence staining in SSc cardiac tissue showed patchy periostin expression in all SSc patients, but not in controls. Furthermore, there was extensive periostin expression even in areas without collagen deposition, while all established fibrotic areas showed colocalization of collagen and periostin. There was no association between periostin levels and interstitial lung disease, pulmonary hypertension or other vascular complications. Conclusion Periostin is elevated in SSc cardiac tissue in vivo and circulating levels of periostin are increased in SSc, correlating with the extent of disease duration, degree of skin fibrosis, and left ventricular structural assessments. Periostin may be a potential biomarker that can provide further pathogenic insight into cardiac fibrosis in SSc. |
| first_indexed | 2024-04-13T20:37:11Z |
| format | Article |
| id | doaj.art-9fe0c029bf0a4440b5c6a045214e65cd |
| institution | Directory Open Access Journal |
| issn | 1478-6362 |
| language | English |
| last_indexed | 2024-04-13T20:37:11Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Arthritis Research & Therapy |
| spelling | doaj.art-9fe0c029bf0a4440b5c6a045214e65cd2022-12-22T02:31:01ZengBMCArthritis Research & Therapy1478-63622022-11-0124111210.1186/s13075-022-02943-2Periostin overexpression in scleroderma cardiac tissue and its utility as a marker for disease complicationsFatima El-Adili0Justin K. Lui1Mortada Najem2Giuseppina Farina3Maria Trojanowska4Flora Sam5Andreea M. Bujor6Division of Rheumatology, Boston University School of MedicineThe Pulmonary Center, Boston University School of MedicineDivision of Rheumatology, Boston University School of MedicineDivision of Rheumatology, Boston University School of MedicineDivision of Rheumatology, Boston University School of MedicineWhitaker Cardiovascular Institute, Boston University School of MedicineDivision of Rheumatology, Boston University School of MedicineAbstract Objective To evaluate the levels of periostin in patients with systemic sclerosis (SSc) and their association with features of systemic sclerosis. Methods The levels of periostin were assessed in the serum of 106 SSc patients and 22 healthy controls and by immunofluorescence staining in cardiac tissue from 4 SSc patients and 4 controls. Serum periostin was measured via enzyme-linked immunosorbent assay. The results were analyzed using Mann-Whitney test or Kruskal-Wallis test followed by Dunn’s multiple comparisons tests and Spearman’s test for correlations. Cardiac tissue from SSc patients and controls was stained for periostin and co-stained for periostin and collagen type I using immunofluorescence. Results Periostin levels were higher in patients with SSc compared to controls and directly correlated to modified Rodnan skin score and echocardiography parameters of left ventricular measurements. Immunofluorescence staining in SSc cardiac tissue showed patchy periostin expression in all SSc patients, but not in controls. Furthermore, there was extensive periostin expression even in areas without collagen deposition, while all established fibrotic areas showed colocalization of collagen and periostin. There was no association between periostin levels and interstitial lung disease, pulmonary hypertension or other vascular complications. Conclusion Periostin is elevated in SSc cardiac tissue in vivo and circulating levels of periostin are increased in SSc, correlating with the extent of disease duration, degree of skin fibrosis, and left ventricular structural assessments. Periostin may be a potential biomarker that can provide further pathogenic insight into cardiac fibrosis in SSc.https://doi.org/10.1186/s13075-022-02943-2SclerodermaPeriostinBiomarkersFibrosis |
| spellingShingle | Fatima El-Adili Justin K. Lui Mortada Najem Giuseppina Farina Maria Trojanowska Flora Sam Andreea M. Bujor Periostin overexpression in scleroderma cardiac tissue and its utility as a marker for disease complications Arthritis Research & Therapy Scleroderma Periostin Biomarkers Fibrosis |
| title | Periostin overexpression in scleroderma cardiac tissue and its utility as a marker for disease complications |
| title_full | Periostin overexpression in scleroderma cardiac tissue and its utility as a marker for disease complications |
| title_fullStr | Periostin overexpression in scleroderma cardiac tissue and its utility as a marker for disease complications |
| title_full_unstemmed | Periostin overexpression in scleroderma cardiac tissue and its utility as a marker for disease complications |
| title_short | Periostin overexpression in scleroderma cardiac tissue and its utility as a marker for disease complications |
| title_sort | periostin overexpression in scleroderma cardiac tissue and its utility as a marker for disease complications |
| topic | Scleroderma Periostin Biomarkers Fibrosis |
| url | https://doi.org/10.1186/s13075-022-02943-2 |
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