A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thaliana
The lignification of plant secondary walls is an important process that provides plants with mechanical support. However, the presence of lignin in the secondary walls affects the readily availability of cellulose required in various industries, including the biofuel, paper, and textile industries....
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1212979/full |
| _version_ | 1827901906888949760 |
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| author | David Charles Simiyu David Charles Simiyu David Charles Simiyu Jin Hoon Jang Jin Hoon Jang Ok Ran Lee Ok Ran Lee |
| author_facet | David Charles Simiyu David Charles Simiyu David Charles Simiyu Jin Hoon Jang Jin Hoon Jang Ok Ran Lee Ok Ran Lee |
| author_sort | David Charles Simiyu |
| collection | DOAJ |
| description | The lignification of plant secondary walls is an important process that provides plants with mechanical support. However, the presence of lignin in the secondary walls affects the readily availability of cellulose required in various industries, including the biofuel, paper, and textile industries. Thus, plants with less lignin are ideal for usage in such industries. Molecular studies have identified genes that regulate plant lignification, including group III plant-specific patatin-related phospholipase genes. Recent studies have reported decreased lignin content when pPLAIIIα, pPLAIIIγ (from Arabidopsis thaliana), and pPLAIIIβ (from Panax ginseng) were overexpressed in Arabidopsis. However, the role played by a closely related gene pPLAIIIδ in lignin biosynthesis has not yet been reported. In this study, we found that overexpression of the pPLAIIIδ significantly reduced the lignin content in secondary cell walls, whereas the silencing of the gene increased secondary walls lignification. Transcript level analysis showed that the key structural and regulatory genes involved in the lignin biosynthesis pathway decreased in overexpression, and increased in plants with silenced pPLAIIIδ. Further analysis revealed that pPLAIIIδ played an influential role in several physiological processes including seed germination, and chlorophyll accumulation. Moreover, the gene also influenced the size of plants and plant organs, including leaves, seeds, and root hairs. Generally, our study provides important insights toward the use of genetic engineering for lignin reduction in plants and provides information about the agronomical and physiological suitability of pPLAIIIδ transgenic plants for utilization in biomass processing industries. |
| first_indexed | 2024-03-12T23:51:27Z |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-12T23:51:27Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-9fe1757dd643498d8bb7cb2a8a0da7c32023-07-13T13:03:21ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-07-011410.3389/fpls.2023.12129791212979A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thalianaDavid Charles Simiyu0David Charles Simiyu1David Charles Simiyu2Jin Hoon Jang3Jin Hoon Jang4Ok Ran Lee5Ok Ran Lee6Department of Applied Plant Science, College of Agriculture and Life Science, Chonnam National University, Gwangju, Republic of KoreaInterdisciplinary Program in IT-Bio Convergence System, Chonnam National University, Gwangju, Republic of KoreaBotany Department, College of Natural and Applied Sciences, University of Dar es Salaam, Dar es Salaam, TanzaniaDepartment of Applied Plant Science, College of Agriculture and Life Science, Chonnam National University, Gwangju, Republic of KoreaInterdisciplinary Program in IT-Bio Convergence System, Chonnam National University, Gwangju, Republic of KoreaDepartment of Applied Plant Science, College of Agriculture and Life Science, Chonnam National University, Gwangju, Republic of KoreaInterdisciplinary Program in IT-Bio Convergence System, Chonnam National University, Gwangju, Republic of KoreaThe lignification of plant secondary walls is an important process that provides plants with mechanical support. However, the presence of lignin in the secondary walls affects the readily availability of cellulose required in various industries, including the biofuel, paper, and textile industries. Thus, plants with less lignin are ideal for usage in such industries. Molecular studies have identified genes that regulate plant lignification, including group III plant-specific patatin-related phospholipase genes. Recent studies have reported decreased lignin content when pPLAIIIα, pPLAIIIγ (from Arabidopsis thaliana), and pPLAIIIβ (from Panax ginseng) were overexpressed in Arabidopsis. However, the role played by a closely related gene pPLAIIIδ in lignin biosynthesis has not yet been reported. In this study, we found that overexpression of the pPLAIIIδ significantly reduced the lignin content in secondary cell walls, whereas the silencing of the gene increased secondary walls lignification. Transcript level analysis showed that the key structural and regulatory genes involved in the lignin biosynthesis pathway decreased in overexpression, and increased in plants with silenced pPLAIIIδ. Further analysis revealed that pPLAIIIδ played an influential role in several physiological processes including seed germination, and chlorophyll accumulation. Moreover, the gene also influenced the size of plants and plant organs, including leaves, seeds, and root hairs. Generally, our study provides important insights toward the use of genetic engineering for lignin reduction in plants and provides information about the agronomical and physiological suitability of pPLAIIIδ transgenic plants for utilization in biomass processing industries.https://www.frontiersin.org/articles/10.3389/fpls.2023.1212979/fullphospholipasesligninchlorophyllspPLAIIIδMGDG |
| spellingShingle | David Charles Simiyu David Charles Simiyu David Charles Simiyu Jin Hoon Jang Jin Hoon Jang Ok Ran Lee Ok Ran Lee A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thaliana Frontiers in Plant Science phospholipases lignin chlorophylls pPLAIIIδ MGDG |
| title | A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thaliana |
| title_full | A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thaliana |
| title_fullStr | A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thaliana |
| title_full_unstemmed | A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thaliana |
| title_short | A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thaliana |
| title_sort | group iii patatin like phospholipase gene pplaiiiδ regulates lignin biosynthesis and influences the rate of seed germination in arabidopsis thaliana |
| topic | phospholipases lignin chlorophylls pPLAIIIδ MGDG |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1212979/full |
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