Organic Farming Favors <i>phoD</i>-Harboring Rhizospheric Bacterial Community and Alkaline Phosphatase Activity in Tropical Agroecosystem
The bacteria harboring <i>phoD</i> encodes alkaline phosphatase (ALP), a secretory enzyme that hydrolyzes organic phosphorous (P) to a usable form in the soil. The impact of farming practices and crop types on <i>phoD</i> bacterial abundance and diversity in tropical agroecos...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/5/1068 |
| _version_ | 1827752322235629568 |
|---|---|
| author | Neha Yashpal Bhardwaj Bhaskar Reddy Suresh Kumar Dubey |
| author_facet | Neha Yashpal Bhardwaj Bhaskar Reddy Suresh Kumar Dubey |
| author_sort | Neha |
| collection | DOAJ |
| description | The bacteria harboring <i>phoD</i> encodes alkaline phosphatase (ALP), a secretory enzyme that hydrolyzes organic phosphorous (P) to a usable form in the soil. The impact of farming practices and crop types on <i>phoD</i> bacterial abundance and diversity in tropical agroecosystems is largely unknown. In this research, the aim was to study the effect of farming practices (organic vs. conventional) and crop types on the <i>phoD</i>-harboring bacterial community. A high-throughput amplicon (<i>phoD</i> gene) sequencing method was employed for the assessment of bacterial diversity and qPCR for <i>phoD</i> gene abundance. Outcomes revealed that soils treated for organic farming have high observed OTUs, ALP activity, and <i>phoD</i> population than soils managed under conventional farming with the trend of maize > chickpea > mustard > soybean vegetated soils. The relative abundance of <i>Rhizobiales</i> exhibited dominance. <i>Ensifer</i>, <i>Bradyrhizobium</i>, <i>Streptomyces</i>, and <i>Pseudomonas</i> were observed as dominant genera in both farming practices. Overall, the study demonstrated that organic farming practice favors the ALP activity, <i>phoD</i> abundance, and OTU richness which varied across crop types with maize crops showing the highest OTUs followed by chickpea, mustard, and least in soybean cropping. |
| first_indexed | 2024-03-11T07:13:50Z |
| format | Article |
| id | doaj.art-9fe1c167160941df8bb9118261e9bf3d |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T07:13:50Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-9fe1c167160941df8bb9118261e9bf3d2023-11-17T08:23:42ZengMDPI AGPlants2223-77472023-02-01125106810.3390/plants12051068Organic Farming Favors <i>phoD</i>-Harboring Rhizospheric Bacterial Community and Alkaline Phosphatase Activity in Tropical AgroecosystemNeha0Yashpal Bhardwaj1Bhaskar Reddy2Suresh Kumar Dubey3Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, IndiaCentre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, IndiaCentre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, IndiaCentre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, IndiaThe bacteria harboring <i>phoD</i> encodes alkaline phosphatase (ALP), a secretory enzyme that hydrolyzes organic phosphorous (P) to a usable form in the soil. The impact of farming practices and crop types on <i>phoD</i> bacterial abundance and diversity in tropical agroecosystems is largely unknown. In this research, the aim was to study the effect of farming practices (organic vs. conventional) and crop types on the <i>phoD</i>-harboring bacterial community. A high-throughput amplicon (<i>phoD</i> gene) sequencing method was employed for the assessment of bacterial diversity and qPCR for <i>phoD</i> gene abundance. Outcomes revealed that soils treated for organic farming have high observed OTUs, ALP activity, and <i>phoD</i> population than soils managed under conventional farming with the trend of maize > chickpea > mustard > soybean vegetated soils. The relative abundance of <i>Rhizobiales</i> exhibited dominance. <i>Ensifer</i>, <i>Bradyrhizobium</i>, <i>Streptomyces</i>, and <i>Pseudomonas</i> were observed as dominant genera in both farming practices. Overall, the study demonstrated that organic farming practice favors the ALP activity, <i>phoD</i> abundance, and OTU richness which varied across crop types with maize crops showing the highest OTUs followed by chickpea, mustard, and least in soybean cropping.https://www.mdpi.com/2223-7747/12/5/1068alkaline phosphatase activityavailable phosphorous<i>phoD</i> abundancefarming practicehigh-throughput sequencing |
| spellingShingle | Neha Yashpal Bhardwaj Bhaskar Reddy Suresh Kumar Dubey Organic Farming Favors <i>phoD</i>-Harboring Rhizospheric Bacterial Community and Alkaline Phosphatase Activity in Tropical Agroecosystem Plants alkaline phosphatase activity available phosphorous <i>phoD</i> abundance farming practice high-throughput sequencing |
| title | Organic Farming Favors <i>phoD</i>-Harboring Rhizospheric Bacterial Community and Alkaline Phosphatase Activity in Tropical Agroecosystem |
| title_full | Organic Farming Favors <i>phoD</i>-Harboring Rhizospheric Bacterial Community and Alkaline Phosphatase Activity in Tropical Agroecosystem |
| title_fullStr | Organic Farming Favors <i>phoD</i>-Harboring Rhizospheric Bacterial Community and Alkaline Phosphatase Activity in Tropical Agroecosystem |
| title_full_unstemmed | Organic Farming Favors <i>phoD</i>-Harboring Rhizospheric Bacterial Community and Alkaline Phosphatase Activity in Tropical Agroecosystem |
| title_short | Organic Farming Favors <i>phoD</i>-Harboring Rhizospheric Bacterial Community and Alkaline Phosphatase Activity in Tropical Agroecosystem |
| title_sort | organic farming favors i phod i harboring rhizospheric bacterial community and alkaline phosphatase activity in tropical agroecosystem |
| topic | alkaline phosphatase activity available phosphorous <i>phoD</i> abundance farming practice high-throughput sequencing |
| url | https://www.mdpi.com/2223-7747/12/5/1068 |
| work_keys_str_mv | AT neha organicfarmingfavorsiphodiharboringrhizosphericbacterialcommunityandalkalinephosphataseactivityintropicalagroecosystem AT yashpalbhardwaj organicfarmingfavorsiphodiharboringrhizosphericbacterialcommunityandalkalinephosphataseactivityintropicalagroecosystem AT bhaskarreddy organicfarmingfavorsiphodiharboringrhizosphericbacterialcommunityandalkalinephosphataseactivityintropicalagroecosystem AT sureshkumardubey organicfarmingfavorsiphodiharboringrhizosphericbacterialcommunityandalkalinephosphataseactivityintropicalagroecosystem |