Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia
Two zoonotic protozoan pathogens, Giardia duodenalis and Toxoplasma gondii, are important causes of waterborne infections in the Quindío region in Colombia. No previous data exist on how contamination occurs at the source for drinking water consumed by the human population in this region. Our aim wa...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Food and Waterborne Parasitology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405676622000324 |
| _version_ | 1811209234670419968 |
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| author | Valeria Alejandra Pinto-Duarte Natalia Marcela Hérnandez-Arango Benyi Juliana Marin-Gallego Paola Andrea Toloza-Beltrán Fabiana María Lora-Suarez Jorge Enrique Gómez-Marín |
| author_facet | Valeria Alejandra Pinto-Duarte Natalia Marcela Hérnandez-Arango Benyi Juliana Marin-Gallego Paola Andrea Toloza-Beltrán Fabiana María Lora-Suarez Jorge Enrique Gómez-Marín |
| author_sort | Valeria Alejandra Pinto-Duarte |
| collection | DOAJ |
| description | Two zoonotic protozoan pathogens, Giardia duodenalis and Toxoplasma gondii, are important causes of waterborne infections in the Quindío region in Colombia. No previous data exist on how contamination occurs at the source for drinking water consumed by the human population in this region. Our aim was to describe the frequency of G. duodenalis and T. gondii DNA in 11 sampling points during a five-month period in water and adjacent soil at the Quindío River basin (Andean region in the central western part of Colombia). The study employed nested PCR for T. gondii, using the B1 gene as the amplification target, and single-round PCR for G. duodenalis assemblage A and assemblage B, amplifying the gdh gene, followed by DNA sequencing. In 50 soil samples, 28% (14/50) were positive for T. gondii. For G. duodenalis, distribution was in equal parts for assemblage A (8%; 4/50) and assemblage B (8%, 4/50). Genotyping of T. gondii sequences showed two soil samples with type I strain, another two samples of soil with type III strain, but most samples were of unidentified strains. In water samples, T. gondii was detected in 9.1% (5/55), G. duodenalis assemblage A in 34.5% (19/55), and G. duodenalis assemblage B in 12.7% (7/55). T. gondii DNA positivity was associated with lower soil temperature (p = 0.0239). Presence of G. duodenalis and T. gondii was evidenced in soil and water samples in the Quindío River basin, indicating soil as the potential source of contamination for the river that it is destined for human consumption. Monitoring these protozoa in drinking water is necessary to prevent public health risks in human populations. |
| first_indexed | 2024-04-12T04:36:01Z |
| format | Article |
| id | doaj.art-9fe47e3705ef4454b7415e7ee04c0142 |
| institution | Directory Open Access Journal |
| issn | 2405-6766 |
| language | English |
| last_indexed | 2024-04-12T04:36:01Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food and Waterborne Parasitology |
| spelling | doaj.art-9fe47e3705ef4454b7415e7ee04c01422022-12-22T03:47:49ZengElsevierFood and Waterborne Parasitology2405-67662022-09-0128e00175Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, ColombiaValeria Alejandra Pinto-Duarte0Natalia Marcela Hérnandez-Arango1Benyi Juliana Marin-Gallego2Paola Andrea Toloza-Beltrán3Fabiana María Lora-Suarez4Jorge Enrique Gómez-Marín5Universidad del Quindío, Molecular Parasitology Study Group (GEPAMOL), Carrera 15, Calle 12N, Armenia, 630003, ColombiaUniversidad del Quindío, Molecular Parasitology Study Group (GEPAMOL), Carrera 15, Calle 12N, Armenia, 630003, ColombiaUniversidad del Quindío, Molecular Parasitology Study Group (GEPAMOL), Carrera 15, Calle 12N, Armenia, 630003, ColombiaUniversidad del Quindío, Molecular Parasitology Study Group (GEPAMOL), Carrera 15, Calle 12N, Armenia, 630003, ColombiaUniversidad del Quindío, Molecular Parasitology Study Group (GEPAMOL), Carrera 15, Calle 12N, Armenia, 630003, ColombiaCorresponding author at: Universidad del Quindío, Molecular Parasitology Study Group (GEPAMOL), Carrera 15, Calle 12N, Armenia, 630003, Colombia.; Universidad del Quindío, Molecular Parasitology Study Group (GEPAMOL), Carrera 15, Calle 12N, Armenia, 630003, ColombiaTwo zoonotic protozoan pathogens, Giardia duodenalis and Toxoplasma gondii, are important causes of waterborne infections in the Quindío region in Colombia. No previous data exist on how contamination occurs at the source for drinking water consumed by the human population in this region. Our aim was to describe the frequency of G. duodenalis and T. gondii DNA in 11 sampling points during a five-month period in water and adjacent soil at the Quindío River basin (Andean region in the central western part of Colombia). The study employed nested PCR for T. gondii, using the B1 gene as the amplification target, and single-round PCR for G. duodenalis assemblage A and assemblage B, amplifying the gdh gene, followed by DNA sequencing. In 50 soil samples, 28% (14/50) were positive for T. gondii. For G. duodenalis, distribution was in equal parts for assemblage A (8%; 4/50) and assemblage B (8%, 4/50). Genotyping of T. gondii sequences showed two soil samples with type I strain, another two samples of soil with type III strain, but most samples were of unidentified strains. In water samples, T. gondii was detected in 9.1% (5/55), G. duodenalis assemblage A in 34.5% (19/55), and G. duodenalis assemblage B in 12.7% (7/55). T. gondii DNA positivity was associated with lower soil temperature (p = 0.0239). Presence of G. duodenalis and T. gondii was evidenced in soil and water samples in the Quindío River basin, indicating soil as the potential source of contamination for the river that it is destined for human consumption. Monitoring these protozoa in drinking water is necessary to prevent public health risks in human populations.http://www.sciencedirect.com/science/article/pii/S2405676622000324Waterborne diseasesSoilToxoplasmaGiardiaColombiaQuindío |
| spellingShingle | Valeria Alejandra Pinto-Duarte Natalia Marcela Hérnandez-Arango Benyi Juliana Marin-Gallego Paola Andrea Toloza-Beltrán Fabiana María Lora-Suarez Jorge Enrique Gómez-Marín Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia Food and Waterborne Parasitology Waterborne diseases Soil Toxoplasma Giardia Colombia Quindío |
| title | Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia |
| title_full | Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia |
| title_fullStr | Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia |
| title_full_unstemmed | Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia |
| title_short | Detection of Giardia duodenalis and Toxoplasma gondii in soil and water samples in the Quindío River basin, Colombia |
| title_sort | detection of giardia duodenalis and toxoplasma gondii in soil and water samples in the quindio river basin colombia |
| topic | Waterborne diseases Soil Toxoplasma Giardia Colombia Quindío |
| url | http://www.sciencedirect.com/science/article/pii/S2405676622000324 |
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