Characterization of immune microenvironment infiltration and m6A regulator-mediated RNA methylation modification patterns in osteoarthritis
BackgroundFew studies have been reported the potential role of N6-methyladenosine (m6A) modification in osteoarthritis (OA). We investigated the patterns of m6A modification in the immune microenvironment of OA.MethodsWe evaluated the m6A modification patterns based on 22 m6A regulators in 139 OA sa...
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| Format: | Article |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1018701/full |
| _version_ | 1811179562521853952 |
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| author | Yulong Ouyang Yuanqing Tu Shuilin Chen Huan Min Zhexu Wen Guihao Zheng Ting Wan Hao Fan Wenzhao Yang Guicai Sun |
| author_facet | Yulong Ouyang Yuanqing Tu Shuilin Chen Huan Min Zhexu Wen Guihao Zheng Ting Wan Hao Fan Wenzhao Yang Guicai Sun |
| author_sort | Yulong Ouyang |
| collection | DOAJ |
| description | BackgroundFew studies have been reported the potential role of N6-methyladenosine (m6A) modification in osteoarthritis (OA). We investigated the patterns of m6A modification in the immune microenvironment of OA.MethodsWe evaluated the m6A modification patterns based on 22 m6A regulators in 139 OA samples and systematically associated these modification patterns with immune cell infiltration characteristics. The function of m6A phenotype-related differentially expressed genes (DEGs) was investigated using gene enrichment analysis. An m6A score model was constructed using principal component analysis (PCA), and an OA prediction model was established based on the key m6A regulators. We used real-time PCR analysis to detect the changes of gene expression in the cell model of OA.ResultsHealthy and OA samples showed significant differences in the expression of m6A regulators. Nine key m6A regulators, two m6A modification patterns, m6A-related genes and two gene clusters were identified. Some m6A regulators had a strong correlation with each other. Gene clusters and m6A clusters have high similarity, and cluster A corresponds to a high m6A score. Immunocytes infiltration differed significantly between the two clusters, with the m6A cluster B and gene cluster B having more types of infiltrating immunocytes than cluster A. The predictive model can also predict the progression of OA through m6A regulators expression. The results of real-time PCR analysis showed that the gene expression in the cell model of OA is similar to that of the m6A cluster B.ConclusionsOur study reveals for the first time the potential regulatory mechanism of m6A modification in the immune microenvironment of OA. This study also sheds new light on the pathogenesis of OA. |
| first_indexed | 2024-04-11T06:36:23Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-11T06:36:23Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj.art-9fe95a309875497ba062d071dffe8bc62022-12-22T04:39:41ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-11-011310.3389/fimmu.2022.10187011018701Characterization of immune microenvironment infiltration and m6A regulator-mediated RNA methylation modification patterns in osteoarthritisYulong Ouyang0Yuanqing Tu1Shuilin Chen2Huan Min3Zhexu Wen4Guihao Zheng5Ting Wan6Hao Fan7Wenzhao Yang8Guicai Sun9Nanchang University, Nanchang, Jiangxi, ChinaNanchang University, Nanchang, Jiangxi, ChinaNanchang University, Nanchang, Jiangxi, ChinaJiangxi Provincial People’s Hospital, Nanchang, Jiangxi, ChinaThe Fourth Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, ChinaShangrao People’s Hospital, Shangrao, Jiangxi, ChinaNanchang University, Nanchang, Jiangxi, ChinaNanchang University, Nanchang, Jiangxi, ChinaNanchang University, Nanchang, Jiangxi, ChinaThe First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, ChinaBackgroundFew studies have been reported the potential role of N6-methyladenosine (m6A) modification in osteoarthritis (OA). We investigated the patterns of m6A modification in the immune microenvironment of OA.MethodsWe evaluated the m6A modification patterns based on 22 m6A regulators in 139 OA samples and systematically associated these modification patterns with immune cell infiltration characteristics. The function of m6A phenotype-related differentially expressed genes (DEGs) was investigated using gene enrichment analysis. An m6A score model was constructed using principal component analysis (PCA), and an OA prediction model was established based on the key m6A regulators. We used real-time PCR analysis to detect the changes of gene expression in the cell model of OA.ResultsHealthy and OA samples showed significant differences in the expression of m6A regulators. Nine key m6A regulators, two m6A modification patterns, m6A-related genes and two gene clusters were identified. Some m6A regulators had a strong correlation with each other. Gene clusters and m6A clusters have high similarity, and cluster A corresponds to a high m6A score. Immunocytes infiltration differed significantly between the two clusters, with the m6A cluster B and gene cluster B having more types of infiltrating immunocytes than cluster A. The predictive model can also predict the progression of OA through m6A regulators expression. The results of real-time PCR analysis showed that the gene expression in the cell model of OA is similar to that of the m6A cluster B.ConclusionsOur study reveals for the first time the potential regulatory mechanism of m6A modification in the immune microenvironment of OA. This study also sheds new light on the pathogenesis of OA.https://www.frontiersin.org/articles/10.3389/fimmu.2022.1018701/fullm6A regulatorimmune microenvironmentosteoarthritisRNA methylationimmunocytes |
| spellingShingle | Yulong Ouyang Yuanqing Tu Shuilin Chen Huan Min Zhexu Wen Guihao Zheng Ting Wan Hao Fan Wenzhao Yang Guicai Sun Characterization of immune microenvironment infiltration and m6A regulator-mediated RNA methylation modification patterns in osteoarthritis Frontiers in Immunology m6A regulator immune microenvironment osteoarthritis RNA methylation immunocytes |
| title | Characterization of immune microenvironment infiltration and m6A regulator-mediated RNA methylation modification patterns in osteoarthritis |
| title_full | Characterization of immune microenvironment infiltration and m6A regulator-mediated RNA methylation modification patterns in osteoarthritis |
| title_fullStr | Characterization of immune microenvironment infiltration and m6A regulator-mediated RNA methylation modification patterns in osteoarthritis |
| title_full_unstemmed | Characterization of immune microenvironment infiltration and m6A regulator-mediated RNA methylation modification patterns in osteoarthritis |
| title_short | Characterization of immune microenvironment infiltration and m6A regulator-mediated RNA methylation modification patterns in osteoarthritis |
| title_sort | characterization of immune microenvironment infiltration and m6a regulator mediated rna methylation modification patterns in osteoarthritis |
| topic | m6A regulator immune microenvironment osteoarthritis RNA methylation immunocytes |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1018701/full |
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