Characterization of a Host-Specific Protein Toxin (Ptr ToxB) from Pyrenophora tritici-repentis
Pyrenophora tritici-repentis, the causal agent of tan spot of wheat, differentially induces tan necrosis and/or chlorosis in wheat. A chlorosis-inducing, host-specific toxin, termed Ptr ToxB (formerly Ptr chlorosis toxin), was purified from the culture filtrates of a race 5 isolate of P. tritici-rep...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1999-08-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.8.728 |
| _version_ | 1811291342666465280 |
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| author | Stephen E. Strelkov Lakhdar Lamari G. Murray Ballance |
| author_facet | Stephen E. Strelkov Lakhdar Lamari G. Murray Ballance |
| author_sort | Stephen E. Strelkov |
| collection | DOAJ |
| description | Pyrenophora tritici-repentis, the causal agent of tan spot of wheat, differentially induces tan necrosis and/or chlorosis in wheat. A chlorosis-inducing, host-specific toxin, termed Ptr ToxB (formerly Ptr chlorosis toxin), was purified from the culture filtrates of a race 5 isolate of P. tritici-repentis. Partial purification was performed by 25 to 80% ammonium sulfate precipitation and passage through a carboxy-methyl-Sephadex C-25 cation exchange column. Final purification was performed by fast performance liquid chromatography, with a Mono S HR 5/5 cation exchanger, followed by size fractionation on a Superose 12 HR 10/30 column. The toxin was shown to be proteinaceous in nature, and purity was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular mass of Ptr ToxB was determined to be 6.61 kDa. The amino acid composition and partial N terminus amino acid sequence of the toxin were also obtained. Ptr ToxB was found to be heat stable, maintaining full toxic activity after 1 h at 55°C. Infiltration of toxin concentrations as low as 14 nM produced chlorosis on susceptible cultivars. |
| first_indexed | 2024-04-13T04:27:48Z |
| format | Article |
| id | doaj.art-9feb41ea9d2e45cda9d266e3d0ea4a0d |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:27:48Z |
| publishDate | 1999-08-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-9feb41ea9d2e45cda9d266e3d0ea4a0d2022-12-22T03:02:27ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061999-08-0112872873210.1094/MPMI.1999.12.8.728Characterization of a Host-Specific Protein Toxin (Ptr ToxB) from Pyrenophora tritici-repentisStephen E. StrelkovLakhdar LamariG. Murray BallancePyrenophora tritici-repentis, the causal agent of tan spot of wheat, differentially induces tan necrosis and/or chlorosis in wheat. A chlorosis-inducing, host-specific toxin, termed Ptr ToxB (formerly Ptr chlorosis toxin), was purified from the culture filtrates of a race 5 isolate of P. tritici-repentis. Partial purification was performed by 25 to 80% ammonium sulfate precipitation and passage through a carboxy-methyl-Sephadex C-25 cation exchange column. Final purification was performed by fast performance liquid chromatography, with a Mono S HR 5/5 cation exchanger, followed by size fractionation on a Superose 12 HR 10/30 column. The toxin was shown to be proteinaceous in nature, and purity was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular mass of Ptr ToxB was determined to be 6.61 kDa. The amino acid composition and partial N terminus amino acid sequence of the toxin were also obtained. Ptr ToxB was found to be heat stable, maintaining full toxic activity after 1 h at 55°C. Infiltration of toxin concentrations as low as 14 nM produced chlorosis on susceptible cultivars.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.8.728Ptr necrosis toxinPtr ToxATriticum aestivum L |
| spellingShingle | Stephen E. Strelkov Lakhdar Lamari G. Murray Ballance Characterization of a Host-Specific Protein Toxin (Ptr ToxB) from Pyrenophora tritici-repentis Molecular Plant-Microbe Interactions Ptr necrosis toxin Ptr ToxA Triticum aestivum L |
| title | Characterization of a Host-Specific Protein Toxin (Ptr ToxB) from Pyrenophora tritici-repentis |
| title_full | Characterization of a Host-Specific Protein Toxin (Ptr ToxB) from Pyrenophora tritici-repentis |
| title_fullStr | Characterization of a Host-Specific Protein Toxin (Ptr ToxB) from Pyrenophora tritici-repentis |
| title_full_unstemmed | Characterization of a Host-Specific Protein Toxin (Ptr ToxB) from Pyrenophora tritici-repentis |
| title_short | Characterization of a Host-Specific Protein Toxin (Ptr ToxB) from Pyrenophora tritici-repentis |
| title_sort | characterization of a host specific protein toxin ptr toxb from pyrenophora tritici repentis |
| topic | Ptr necrosis toxin Ptr ToxA Triticum aestivum L |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.8.728 |
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