A CASE OF ANTI PP1PK IN AN ELDERLY MAN FROM SOUTH BRAZIL
The antigens in the P1PK blood group system including P, P1, and Pk are carbohydrate. More than 99.9% of the population worldwide has the P1 (P+P1+Pk+) or P2 (P+P1-Pk+). Individuals in which P, P1, and Pk are absent are known as having the rare p or P-null phenotype with a rate of five in a group of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Hematology, Transfusion and Cell Therapy |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2531137923013251 |
| _version_ | 1797654844603891712 |
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| author | JG Bohatczuk C Prochaska FL Guimaraes K Cruz |
| author_facet | JG Bohatczuk C Prochaska FL Guimaraes K Cruz |
| author_sort | JG Bohatczuk |
| collection | DOAJ |
| description | The antigens in the P1PK blood group system including P, P1, and Pk are carbohydrate. More than 99.9% of the population worldwide has the P1 (P+P1+Pk+) or P2 (P+P1-Pk+). Individuals in which P, P1, and Pk are absent are known as having the rare p or P-null phenotype with a rate of five in a group of one million people. As in the ABO system, naturally occurring antibodies of the immunoglobulin IgM or IgG class, against the missing carbohydrate structures, can be present in the sera of people lacking the corresponding antigen. Yet the behavior of different antibodies involved are not the same. While Anti-P1 is generally a weak and cold-reactive antibody not implicated in Hemolytic Transfusion Reaction (HTR) or hemolytic disease of the fetus and newborn, anti-PP1Pk or the formerly known anti-Tja or anti p has the ability to cause immediate hemolytic transfusion reactions and recurrent miscarriages, mainly in the first half of pregnancy. Unlike to most articles dealing with anti-PP1Pk that involve women of reproductive age, the present article deals with an old man. Case: In June 2023, a 70-year-old man was referred to Hospital from Guarapuava, Parana, South Brazil, for an orthopedic surgery having two Red Blood Cells (RBCs) concentrates as surgical reserve. Screening studies carried out at the hospital using tube method showed his blood type was group O+ with antibodies screening test positive and the cross-match not compatible for the RBCs tested. Patient related had no known history of transfusions. A whole blood sample was sent to Hemocentro Regional de Guarapuava for complementary tests. Phenotyping, antibody screening, ABO/D typing, Direct Antiglobulin Test (DAT), antibody identification tests and three crossmatches using patient's serum and RBCs from two others blood donors beyond a third using the own patient's blood were performed employing column agglutination method (Bio-Rad, Switzerland). Results: Tests confirmed patient O+, phenotype C+, c+, E-, e+, K-, k+, Kp(a-b+), Jk(a+b+),Le(a-b+), Lu(a-b+), P1-, M+, N+, S+, s+, Fy(a+b+), Di(a-b+). Antibody screening tests and crossmatches showed pan-agglutination of strength (3+) but Negative reactions to DAT and to the autologous RBCs were observed. In view of these inconclusive results was collected a new patient's whole blood sample and sent to Hemepar and Biorad Latin America Laboratory in Paraná and Minas Gerais states. Patient's blood tested with anti-PP1Pk antibodies resulted negative. Also the patient's serum tested with known p[(Tj(a-)] RBCs resulted negative. Discussion: The case's patient fortunately didn't have any bleeding during his surgery and unfortunately died five days later due to other complications associated to his ilness. And also nobody of his family was invited to attend Hemocentro to investigate other P-null members. But if blood was really necessary it would be hard finding available P-null donors mainly at one saturday's evening when the surgery was done. Conclusions: The patient has p phenotype and antibodies with specificity anti-PP1Pk in the serum. In general patients and donors with anti-PP1Pk should be careful to avoid any situations in which could need blood transfusions. Women having planes for future pregnancies should consider the risks and the need of close monitoring during pregnancy. |
| first_indexed | 2024-03-11T17:05:33Z |
| format | Article |
| id | doaj.art-9febc9392b73478a8d6481b54fa0b598 |
| institution | Directory Open Access Journal |
| issn | 2531-1379 |
| language | English |
| last_indexed | 2024-03-11T17:05:33Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Hematology, Transfusion and Cell Therapy |
| spelling | doaj.art-9febc9392b73478a8d6481b54fa0b5982023-10-20T06:45:20ZengElsevierHematology, Transfusion and Cell Therapy2531-13792023-10-0145S631A CASE OF ANTI PP1PK IN AN ELDERLY MAN FROM SOUTH BRAZILJG Bohatczuk0C Prochaska1FL Guimaraes2K Cruz3Hemocentro Regional de Guarapuava, Guarapuava, PR, BrazilCentro de Hematologia e Hemoterapia do Parana (Hemepar), Curitiba, PR, BrazilBio-rad Latino America ‒ Laboratório Central, Lagoa Santa, MG, BrazilBio-rad Latino America ‒ Laboratório Central, Lagoa Santa, MG, BrazilThe antigens in the P1PK blood group system including P, P1, and Pk are carbohydrate. More than 99.9% of the population worldwide has the P1 (P+P1+Pk+) or P2 (P+P1-Pk+). Individuals in which P, P1, and Pk are absent are known as having the rare p or P-null phenotype with a rate of five in a group of one million people. As in the ABO system, naturally occurring antibodies of the immunoglobulin IgM or IgG class, against the missing carbohydrate structures, can be present in the sera of people lacking the corresponding antigen. Yet the behavior of different antibodies involved are not the same. While Anti-P1 is generally a weak and cold-reactive antibody not implicated in Hemolytic Transfusion Reaction (HTR) or hemolytic disease of the fetus and newborn, anti-PP1Pk or the formerly known anti-Tja or anti p has the ability to cause immediate hemolytic transfusion reactions and recurrent miscarriages, mainly in the first half of pregnancy. Unlike to most articles dealing with anti-PP1Pk that involve women of reproductive age, the present article deals with an old man. Case: In June 2023, a 70-year-old man was referred to Hospital from Guarapuava, Parana, South Brazil, for an orthopedic surgery having two Red Blood Cells (RBCs) concentrates as surgical reserve. Screening studies carried out at the hospital using tube method showed his blood type was group O+ with antibodies screening test positive and the cross-match not compatible for the RBCs tested. Patient related had no known history of transfusions. A whole blood sample was sent to Hemocentro Regional de Guarapuava for complementary tests. Phenotyping, antibody screening, ABO/D typing, Direct Antiglobulin Test (DAT), antibody identification tests and three crossmatches using patient's serum and RBCs from two others blood donors beyond a third using the own patient's blood were performed employing column agglutination method (Bio-Rad, Switzerland). Results: Tests confirmed patient O+, phenotype C+, c+, E-, e+, K-, k+, Kp(a-b+), Jk(a+b+),Le(a-b+), Lu(a-b+), P1-, M+, N+, S+, s+, Fy(a+b+), Di(a-b+). Antibody screening tests and crossmatches showed pan-agglutination of strength (3+) but Negative reactions to DAT and to the autologous RBCs were observed. In view of these inconclusive results was collected a new patient's whole blood sample and sent to Hemepar and Biorad Latin America Laboratory in Paraná and Minas Gerais states. Patient's blood tested with anti-PP1Pk antibodies resulted negative. Also the patient's serum tested with known p[(Tj(a-)] RBCs resulted negative. Discussion: The case's patient fortunately didn't have any bleeding during his surgery and unfortunately died five days later due to other complications associated to his ilness. And also nobody of his family was invited to attend Hemocentro to investigate other P-null members. But if blood was really necessary it would be hard finding available P-null donors mainly at one saturday's evening when the surgery was done. Conclusions: The patient has p phenotype and antibodies with specificity anti-PP1Pk in the serum. In general patients and donors with anti-PP1Pk should be careful to avoid any situations in which could need blood transfusions. Women having planes for future pregnancies should consider the risks and the need of close monitoring during pregnancy.http://www.sciencedirect.com/science/article/pii/S2531137923013251 |
| spellingShingle | JG Bohatczuk C Prochaska FL Guimaraes K Cruz A CASE OF ANTI PP1PK IN AN ELDERLY MAN FROM SOUTH BRAZIL Hematology, Transfusion and Cell Therapy |
| title | A CASE OF ANTI PP1PK IN AN ELDERLY MAN FROM SOUTH BRAZIL |
| title_full | A CASE OF ANTI PP1PK IN AN ELDERLY MAN FROM SOUTH BRAZIL |
| title_fullStr | A CASE OF ANTI PP1PK IN AN ELDERLY MAN FROM SOUTH BRAZIL |
| title_full_unstemmed | A CASE OF ANTI PP1PK IN AN ELDERLY MAN FROM SOUTH BRAZIL |
| title_short | A CASE OF ANTI PP1PK IN AN ELDERLY MAN FROM SOUTH BRAZIL |
| title_sort | case of anti pp1pk in an elderly man from south brazil |
| url | http://www.sciencedirect.com/science/article/pii/S2531137923013251 |
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