Combining high intensity ultrasound and experimental design to improve carotenoid extraction efficiency from Buriti (Mauritia flexuosa)
Buriti (Mauritia flexuosa L.) is a significant source of carotenoids, but these compounds have been extracted using laborious and low-effective methods. The present work evaluated the high-intensity ultrasound combined with a chemometric approach to developing an optimal extraction method of caroten...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Ultrasonics Sonochemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417722001699 |
| _version_ | 1818243752439840768 |
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| author | Darlisson Slag Neri Silva Matheus de Sousa Silva Tiago Linus Silva Coelho Clecio Dantas Cícero Alves Lopes Júnior Naise Mary Caldas Edivan Carvalho Vieira |
| author_facet | Darlisson Slag Neri Silva Matheus de Sousa Silva Tiago Linus Silva Coelho Clecio Dantas Cícero Alves Lopes Júnior Naise Mary Caldas Edivan Carvalho Vieira |
| author_sort | Darlisson Slag Neri Silva |
| collection | DOAJ |
| description | Buriti (Mauritia flexuosa L.) is a significant source of carotenoids, but these compounds have been extracted using laborious and low-effective methods. The present work evaluated the high-intensity ultrasound combined with a chemometric approach to developing an optimal extraction method of carotenoids from buriti pulp. The multivariate optimization was carried out through two steps. First, a simplex-lattice mixture design was used to optimize the extractor solution finding higher extraction yield (903 ± 21 µg g−1) with the acetone:ethanol (75/25) mixture. After, sample mass (80 mg) and sonication time (30 min) were optimized applying central composite design (CCD) which provided a 14% improvement in the extraction method yield. So, the total carotenoid content (TCC) with optimal extraction conditions was 1026 ± 13 µg g−1 which is almost twice the yield of methods known in the literature for buriti. The RP-HPLC-DAD analysis revealed that the carotenoids are gently extracted and β-carotene is the major compound in the extracts. To confirm the accuracy, buriti samples spiked with β-carotene standard and the developed method showed recovery >84% and precision <6.5%. Furthermore, the optimized ultrasound-assisted extraction (UAE) method was applied to other samples (tomato, guava, carrot, mango, acerola, papaya, and pumpkin) and presented a yield to 5.5-fold higher when compared to the reported methods indicating high robustness. Based on results, the UAE method developed has demonstrated feasibility and reliability for the study of carotenoids in buriti pulp as well as in other plant matrices with high biological relevance. |
| first_indexed | 2024-12-12T14:06:07Z |
| format | Article |
| id | doaj.art-9fecfee59d3d4b8ea9a28a282d11bd84 |
| institution | Directory Open Access Journal |
| issn | 1350-4177 |
| language | English |
| last_indexed | 2024-12-12T14:06:07Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj.art-9fecfee59d3d4b8ea9a28a282d11bd842022-12-22T00:22:12ZengElsevierUltrasonics Sonochemistry1350-41772022-08-0188106076Combining high intensity ultrasound and experimental design to improve carotenoid extraction efficiency from Buriti (Mauritia flexuosa)Darlisson Slag Neri Silva0Matheus de Sousa Silva1Tiago Linus Silva Coelho2Clecio Dantas3Cícero Alves Lopes Júnior4Naise Mary Caldas5Edivan Carvalho Vieira6Grupo de Instrumentação Analítica e Preparo de Amostra (GRIAPA), Department of Chemistry, Federal University of Piauí – UFPI, 64049-550 Teresina, Piauí, BrazilGrupo de Instrumentação Analítica e Preparo de Amostra (GRIAPA), Department of Chemistry, Federal University of Piauí – UFPI, 64049-550 Teresina, Piauí, BrazilGrupo de Instrumentação Analítica e Preparo de Amostra (GRIAPA), Department of Chemistry, Federal University of Piauí – UFPI, 64049-550 Teresina, Piauí, BrazilLaboratório de Química Computacional Inorgânica e Quimiometria – (LQCINMETRIA), State University of Maranhão - UEMA, 65604-380 Caxias, Maranhão, BrazilGrupo de Estudos em Bioanalítica – GEBIO, Department of Chemistry, Federal University of Piauí, 64049-550 Teresina, PI, Brazil; Institute for Chemistry, TESLA – Analytical Chemistry, University of Graz, Universitätsplatz 1/I, 8010 Graz, Austria; Corresponding authors at: Institute for Chemistry, TESLA – Analytical Chemistry, University of Graz, Universitätsplatz 1/I, 8010 Graz, Austria. Email address: cicero.lopes@uni-graz.at (C.A. Lopes Júnior).Grupo de Instrumentação Analítica e Preparo de Amostra (GRIAPA), Department of Chemistry, Federal University of Piauí – UFPI, 64049-550 Teresina, Piauí, Brazil; Corresponding authors at: Institute for Chemistry, TESLA – Analytical Chemistry, University of Graz, Universitätsplatz 1/I, 8010 Graz, Austria. Email address: cicero.lopes@uni-graz.at (C.A. Lopes Júnior).Grupo de Instrumentação Analítica e Preparo de Amostra (GRIAPA), Department of Chemistry, Federal University of Piauí – UFPI, 64049-550 Teresina, Piauí, Brazil; Corresponding authors at: Institute for Chemistry, TESLA – Analytical Chemistry, University of Graz, Universitätsplatz 1/I, 8010 Graz, Austria. Email address: cicero.lopes@uni-graz.at (C.A. Lopes Júnior).Buriti (Mauritia flexuosa L.) is a significant source of carotenoids, but these compounds have been extracted using laborious and low-effective methods. The present work evaluated the high-intensity ultrasound combined with a chemometric approach to developing an optimal extraction method of carotenoids from buriti pulp. The multivariate optimization was carried out through two steps. First, a simplex-lattice mixture design was used to optimize the extractor solution finding higher extraction yield (903 ± 21 µg g−1) with the acetone:ethanol (75/25) mixture. After, sample mass (80 mg) and sonication time (30 min) were optimized applying central composite design (CCD) which provided a 14% improvement in the extraction method yield. So, the total carotenoid content (TCC) with optimal extraction conditions was 1026 ± 13 µg g−1 which is almost twice the yield of methods known in the literature for buriti. The RP-HPLC-DAD analysis revealed that the carotenoids are gently extracted and β-carotene is the major compound in the extracts. To confirm the accuracy, buriti samples spiked with β-carotene standard and the developed method showed recovery >84% and precision <6.5%. Furthermore, the optimized ultrasound-assisted extraction (UAE) method was applied to other samples (tomato, guava, carrot, mango, acerola, papaya, and pumpkin) and presented a yield to 5.5-fold higher when compared to the reported methods indicating high robustness. Based on results, the UAE method developed has demonstrated feasibility and reliability for the study of carotenoids in buriti pulp as well as in other plant matrices with high biological relevance.http://www.sciencedirect.com/science/article/pii/S1350417722001699BuritiCarotenoidsChemometricsExperimental designsUltrasound-assisted extraction |
| spellingShingle | Darlisson Slag Neri Silva Matheus de Sousa Silva Tiago Linus Silva Coelho Clecio Dantas Cícero Alves Lopes Júnior Naise Mary Caldas Edivan Carvalho Vieira Combining high intensity ultrasound and experimental design to improve carotenoid extraction efficiency from Buriti (Mauritia flexuosa) Ultrasonics Sonochemistry Buriti Carotenoids Chemometrics Experimental designs Ultrasound-assisted extraction |
| title | Combining high intensity ultrasound and experimental design to improve carotenoid extraction efficiency from Buriti (Mauritia flexuosa) |
| title_full | Combining high intensity ultrasound and experimental design to improve carotenoid extraction efficiency from Buriti (Mauritia flexuosa) |
| title_fullStr | Combining high intensity ultrasound and experimental design to improve carotenoid extraction efficiency from Buriti (Mauritia flexuosa) |
| title_full_unstemmed | Combining high intensity ultrasound and experimental design to improve carotenoid extraction efficiency from Buriti (Mauritia flexuosa) |
| title_short | Combining high intensity ultrasound and experimental design to improve carotenoid extraction efficiency from Buriti (Mauritia flexuosa) |
| title_sort | combining high intensity ultrasound and experimental design to improve carotenoid extraction efficiency from buriti mauritia flexuosa |
| topic | Buriti Carotenoids Chemometrics Experimental designs Ultrasound-assisted extraction |
| url | http://www.sciencedirect.com/science/article/pii/S1350417722001699 |
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